Effective coupling of rapid freeze-quench to high-frequency electron paramagnetic resonance

We report an easy, efficient and reproducible way to prepare Rapid-Freeze-Quench samples in sub-millimeter capillaries and load these into the probe head of a 275 GHz Electron Paramagnetic Resonance spectrometer. Kinetic data obtained for the binding reaction of azide to myoglobin demonstrate the fe...

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Autores principales: Panarelli, E. Gabriele, van der Meer, Harmen, Gast, Peter, Groenen, Edgar J J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7213726/
https://www.ncbi.nlm.nih.gov/pubmed/32392255
http://dx.doi.org/10.1371/journal.pone.0232555
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author Panarelli, E. Gabriele
van der Meer, Harmen
Gast, Peter
Groenen, Edgar J J.
author_facet Panarelli, E. Gabriele
van der Meer, Harmen
Gast, Peter
Groenen, Edgar J J.
author_sort Panarelli, E. Gabriele
collection PubMed
description We report an easy, efficient and reproducible way to prepare Rapid-Freeze-Quench samples in sub-millimeter capillaries and load these into the probe head of a 275 GHz Electron Paramagnetic Resonance spectrometer. Kinetic data obtained for the binding reaction of azide to myoglobin demonstrate the feasibility of the method for high-frequency EPR. Experiments on the same samples at 9.5 GHz show that only a single series of Rapid-Freeze-Quench samples is required for studies at multiple microwave frequencies.
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spelling pubmed-72137262020-05-26 Effective coupling of rapid freeze-quench to high-frequency electron paramagnetic resonance Panarelli, E. Gabriele van der Meer, Harmen Gast, Peter Groenen, Edgar J J. PLoS One Research Article We report an easy, efficient and reproducible way to prepare Rapid-Freeze-Quench samples in sub-millimeter capillaries and load these into the probe head of a 275 GHz Electron Paramagnetic Resonance spectrometer. Kinetic data obtained for the binding reaction of azide to myoglobin demonstrate the feasibility of the method for high-frequency EPR. Experiments on the same samples at 9.5 GHz show that only a single series of Rapid-Freeze-Quench samples is required for studies at multiple microwave frequencies. Public Library of Science 2020-05-11 /pmc/articles/PMC7213726/ /pubmed/32392255 http://dx.doi.org/10.1371/journal.pone.0232555 Text en © 2020 Panarelli et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Panarelli, E. Gabriele
van der Meer, Harmen
Gast, Peter
Groenen, Edgar J J.
Effective coupling of rapid freeze-quench to high-frequency electron paramagnetic resonance
title Effective coupling of rapid freeze-quench to high-frequency electron paramagnetic resonance
title_full Effective coupling of rapid freeze-quench to high-frequency electron paramagnetic resonance
title_fullStr Effective coupling of rapid freeze-quench to high-frequency electron paramagnetic resonance
title_full_unstemmed Effective coupling of rapid freeze-quench to high-frequency electron paramagnetic resonance
title_short Effective coupling of rapid freeze-quench to high-frequency electron paramagnetic resonance
title_sort effective coupling of rapid freeze-quench to high-frequency electron paramagnetic resonance
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7213726/
https://www.ncbi.nlm.nih.gov/pubmed/32392255
http://dx.doi.org/10.1371/journal.pone.0232555
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