A Microfluidic Device for Imaging Samples from Microbial Suspension Cultures

• slows and concentrates microbial cells, specifically budding yeast, sampled from suspension culture and improves imaging of individual cells by concentrating them in a single focal plane; • provides imaging quality and temporal resolution that is capable of monitoring dynamic spatiotemporal proces...

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Detalles Bibliográficos
Autores principales: Letourneau, Alexander, Kegel, Jack, Al-Ramahi, Jehad, Yachinich, Emily, Krause, Harris B., Stewart, Cameron J., McClean, Megan N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Immunology and Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7214938/
https://www.ncbi.nlm.nih.gov/pubmed/32420047
http://dx.doi.org/10.1016/j.mex.2020.100891
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author Letourneau, Alexander
Kegel, Jack
Al-Ramahi, Jehad
Yachinich, Emily
Krause, Harris B.
Stewart, Cameron J.
McClean, Megan N.
author_facet Letourneau, Alexander
Kegel, Jack
Al-Ramahi, Jehad
Yachinich, Emily
Krause, Harris B.
Stewart, Cameron J.
McClean, Megan N.
author_sort Letourneau, Alexander
collection PubMed
description • slows and concentrates microbial cells, specifically budding yeast, sampled from suspension culture and improves imaging of individual cells by concentrating them in a single focal plane; • provides imaging quality and temporal resolution that is capable of monitoring dynamic spatiotemporal processes, such as nuclear localization of a protein; • is inexpensive and simple enough to be fabricated and used in laboratories equipped for standard molecular and cellular biology.
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spelling pubmed-72149382020-05-15 A Microfluidic Device for Imaging Samples from Microbial Suspension Cultures Letourneau, Alexander Kegel, Jack Al-Ramahi, Jehad Yachinich, Emily Krause, Harris B. Stewart, Cameron J. McClean, Megan N. MethodsX Immunology and Microbiology • slows and concentrates microbial cells, specifically budding yeast, sampled from suspension culture and improves imaging of individual cells by concentrating them in a single focal plane; • provides imaging quality and temporal resolution that is capable of monitoring dynamic spatiotemporal processes, such as nuclear localization of a protein; • is inexpensive and simple enough to be fabricated and used in laboratories equipped for standard molecular and cellular biology. Elsevier 2020-04-24 /pmc/articles/PMC7214938/ /pubmed/32420047 http://dx.doi.org/10.1016/j.mex.2020.100891 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Immunology and Microbiology
Letourneau, Alexander
Kegel, Jack
Al-Ramahi, Jehad
Yachinich, Emily
Krause, Harris B.
Stewart, Cameron J.
McClean, Megan N.
A Microfluidic Device for Imaging Samples from Microbial Suspension Cultures
title A Microfluidic Device for Imaging Samples from Microbial Suspension Cultures
title_full A Microfluidic Device for Imaging Samples from Microbial Suspension Cultures
title_fullStr A Microfluidic Device for Imaging Samples from Microbial Suspension Cultures
title_full_unstemmed A Microfluidic Device for Imaging Samples from Microbial Suspension Cultures
title_short A Microfluidic Device for Imaging Samples from Microbial Suspension Cultures
title_sort microfluidic device for imaging samples from microbial suspension cultures
topic Immunology and Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7214938/
https://www.ncbi.nlm.nih.gov/pubmed/32420047
http://dx.doi.org/10.1016/j.mex.2020.100891
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