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Comparison Between O and OH Intermediates of Cytochrome c Oxidase Studied by FTIR Spectroscopy

Cytochrome c oxidase is terminal enzyme in the respiratory chain of mitochondria and many aerobic bacteria. It catalyzes reduction of oxygen to water. During its catalysis, CcO proceeds through several quite stable intermediates (R, A, PR/M, O/OH, E/EH). This work is concentrated on the elucidation...

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Autores principales: Gorbikova, Elena, Kalendar, Ruslan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215072/
https://www.ncbi.nlm.nih.gov/pubmed/32432087
http://dx.doi.org/10.3389/fchem.2020.00387
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author Gorbikova, Elena
Kalendar, Ruslan
author_facet Gorbikova, Elena
Kalendar, Ruslan
author_sort Gorbikova, Elena
collection PubMed
description Cytochrome c oxidase is terminal enzyme in the respiratory chain of mitochondria and many aerobic bacteria. It catalyzes reduction of oxygen to water. During its catalysis, CcO proceeds through several quite stable intermediates (R, A, PR/M, O/OH, E/EH). This work is concentrated on the elucidation of the differences between structures of oxidized intermediates O and O(H) in different CcO variants and at different pH values. Oxidized intermediates of wild type and mutated CcO from Paracoccus denitrificans were studied by means of static and time-resolved Fourier-transform infrared spectroscopy in acidic and alkaline conditions in the infrared region 1800–1000 cm(−1). No reasonable differences were found between all variants in these conditions, and in this spectral region. This finding means that the binuclear center of oxygen reduction keeps a very similar structure and holds the same ligands in the studied conditions. The further investigation in search of differences should be performed in the 4000–2000 cm(−1) IR region where water ligands absorb.
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spelling pubmed-72150722020-05-19 Comparison Between O and OH Intermediates of Cytochrome c Oxidase Studied by FTIR Spectroscopy Gorbikova, Elena Kalendar, Ruslan Front Chem Chemistry Cytochrome c oxidase is terminal enzyme in the respiratory chain of mitochondria and many aerobic bacteria. It catalyzes reduction of oxygen to water. During its catalysis, CcO proceeds through several quite stable intermediates (R, A, PR/M, O/OH, E/EH). This work is concentrated on the elucidation of the differences between structures of oxidized intermediates O and O(H) in different CcO variants and at different pH values. Oxidized intermediates of wild type and mutated CcO from Paracoccus denitrificans were studied by means of static and time-resolved Fourier-transform infrared spectroscopy in acidic and alkaline conditions in the infrared region 1800–1000 cm(−1). No reasonable differences were found between all variants in these conditions, and in this spectral region. This finding means that the binuclear center of oxygen reduction keeps a very similar structure and holds the same ligands in the studied conditions. The further investigation in search of differences should be performed in the 4000–2000 cm(−1) IR region where water ligands absorb. Frontiers Media S.A. 2020-05-05 /pmc/articles/PMC7215072/ /pubmed/32432087 http://dx.doi.org/10.3389/fchem.2020.00387 Text en Copyright © 2020 Gorbikova and Kalendar. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Gorbikova, Elena
Kalendar, Ruslan
Comparison Between O and OH Intermediates of Cytochrome c Oxidase Studied by FTIR Spectroscopy
title Comparison Between O and OH Intermediates of Cytochrome c Oxidase Studied by FTIR Spectroscopy
title_full Comparison Between O and OH Intermediates of Cytochrome c Oxidase Studied by FTIR Spectroscopy
title_fullStr Comparison Between O and OH Intermediates of Cytochrome c Oxidase Studied by FTIR Spectroscopy
title_full_unstemmed Comparison Between O and OH Intermediates of Cytochrome c Oxidase Studied by FTIR Spectroscopy
title_short Comparison Between O and OH Intermediates of Cytochrome c Oxidase Studied by FTIR Spectroscopy
title_sort comparison between o and oh intermediates of cytochrome c oxidase studied by ftir spectroscopy
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215072/
https://www.ncbi.nlm.nih.gov/pubmed/32432087
http://dx.doi.org/10.3389/fchem.2020.00387
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