Cargando…

Age-related and sex-specific effects on architectural properties and biomechanical response of the C57BL/6N mouse femur, tibia and ulna

Aging is known to reduce bone quality and bone strength. We sought to determine how aging affects the biomechanical and architectural properties of various long bones, and if sex influences age related differences/changes. While researchers have extensively studied these changes in individual bones...

Descripción completa

Detalles Bibliográficos
Autores principales: Mumtaz, Hammad, Dallas, Mark, Begonia, Mark, Lara-Castillo, Nuria, Scott, JoAnna M., Johnson, Mark L., Ganesh, Thiagarajan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215114/
https://www.ncbi.nlm.nih.gov/pubmed/32420415
http://dx.doi.org/10.1016/j.bonr.2020.100266
_version_ 1783532111195537408
author Mumtaz, Hammad
Dallas, Mark
Begonia, Mark
Lara-Castillo, Nuria
Scott, JoAnna M.
Johnson, Mark L.
Ganesh, Thiagarajan
author_facet Mumtaz, Hammad
Dallas, Mark
Begonia, Mark
Lara-Castillo, Nuria
Scott, JoAnna M.
Johnson, Mark L.
Ganesh, Thiagarajan
author_sort Mumtaz, Hammad
collection PubMed
description Aging is known to reduce bone quality and bone strength. We sought to determine how aging affects the biomechanical and architectural properties of various long bones, and if sex influences age related differences/changes. While researchers have extensively studied these changes in individual bones of mice, there is no comprehensive study of the changes in the bones from the same mice to study the changes with aging. We performed three point bending tests and microcomputed tomography (microCT) analysis on femurs, tibiae and ulnae. Three point bending tests were utilized to calculate biomechanical parameters and imaging was also performed using high resolution microCT to reveal both cortical and trabecular microarchitecture C57BL/6N mice were divided into three age groups: 6, 12 and 22 months. Each age and sex group consisted of 6–7 mice. The ultimate load to failure (UL), elastic stiffness (ES), modulus of elasticity (E) and the moment of inertia about bending axis (MOI) for each bone was calculated using three point bending test. MicroCT scans of all the bones were analyzed to determine cortical bone volume per tissue volume (C.BV/TV), trabecular bone volume per tissue volume (Tb.BV/TV), cortical bone area (B.Ar) using CTAn's microCT analysis and tested for correlation with the biomechanical parameters. Mean (standard error) values of UL in femur decreased from 19.8(0.6) N to 12.8(1.1) N (p < .01) and 17.9(0.6) N to 14.6(1.0) N (p = .02) from 6 to 22 months groups in males and females respectively. Similarly, UL in tibia decreased from 19.8(0.5) N to 14.3(0.2) N (p < .01) and 14.4(0.6) N to 9.5(1.0) N (p < .01) from 6 to 22 months group in males and females respectively. ES in femur decreased from 113.2(7) N/mm to 69.6(6.7) N/mm (p < .01) from 6 to 22 months in males only. ES in tibia decreased from 78.6(3.2) N/mm to 65.0(2.3) N/mm (p = .01) and 53.1(2.9) N/mm to 44.0(1.7) N/mm (p = .02) from 6 to 22 months in males and females respectively. Interestingly, ES in ulna increased from 8.2(0.8) N/mm to 10.9(1.0) N/mm (p = .051) from 6 to 22 months of age in females only. E in femur decreased from 4.0(0.4) GPa to 2.8(0.2) GPa (p = .01) and 6.7(0.5) GPa to 4.5(0.4) GPa (p = .01) from 6 to 22 months of age in males and females respectively while tibia showed no change. However, E in ulna increased from 7.0(0.8) GPa to 11.0(1.1) GPa (p = .01) from 6 to 22 months of age in females only. Changes in age and sex-related bone properties were more pronounced in the femur and tibia, while the ulna showed fewer overall differences. Most of the changes were observed in biomechanical compared to architectural properties and female bones are more severely affected by aging. In conclusion, our data demonstrate that care must be taken to describe bone site and sex-specific, rather than making broad generalizations when describing age-related changes on the biomechanical and architectural properties of the skeleton.
format Online
Article
Text
id pubmed-7215114
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-72151142020-05-15 Age-related and sex-specific effects on architectural properties and biomechanical response of the C57BL/6N mouse femur, tibia and ulna Mumtaz, Hammad Dallas, Mark Begonia, Mark Lara-Castillo, Nuria Scott, JoAnna M. Johnson, Mark L. Ganesh, Thiagarajan Bone Rep Article Aging is known to reduce bone quality and bone strength. We sought to determine how aging affects the biomechanical and architectural properties of various long bones, and if sex influences age related differences/changes. While researchers have extensively studied these changes in individual bones of mice, there is no comprehensive study of the changes in the bones from the same mice to study the changes with aging. We performed three point bending tests and microcomputed tomography (microCT) analysis on femurs, tibiae and ulnae. Three point bending tests were utilized to calculate biomechanical parameters and imaging was also performed using high resolution microCT to reveal both cortical and trabecular microarchitecture C57BL/6N mice were divided into three age groups: 6, 12 and 22 months. Each age and sex group consisted of 6–7 mice. The ultimate load to failure (UL), elastic stiffness (ES), modulus of elasticity (E) and the moment of inertia about bending axis (MOI) for each bone was calculated using three point bending test. MicroCT scans of all the bones were analyzed to determine cortical bone volume per tissue volume (C.BV/TV), trabecular bone volume per tissue volume (Tb.BV/TV), cortical bone area (B.Ar) using CTAn's microCT analysis and tested for correlation with the biomechanical parameters. Mean (standard error) values of UL in femur decreased from 19.8(0.6) N to 12.8(1.1) N (p < .01) and 17.9(0.6) N to 14.6(1.0) N (p = .02) from 6 to 22 months groups in males and females respectively. Similarly, UL in tibia decreased from 19.8(0.5) N to 14.3(0.2) N (p < .01) and 14.4(0.6) N to 9.5(1.0) N (p < .01) from 6 to 22 months group in males and females respectively. ES in femur decreased from 113.2(7) N/mm to 69.6(6.7) N/mm (p < .01) from 6 to 22 months in males only. ES in tibia decreased from 78.6(3.2) N/mm to 65.0(2.3) N/mm (p = .01) and 53.1(2.9) N/mm to 44.0(1.7) N/mm (p = .02) from 6 to 22 months in males and females respectively. Interestingly, ES in ulna increased from 8.2(0.8) N/mm to 10.9(1.0) N/mm (p = .051) from 6 to 22 months of age in females only. E in femur decreased from 4.0(0.4) GPa to 2.8(0.2) GPa (p = .01) and 6.7(0.5) GPa to 4.5(0.4) GPa (p = .01) from 6 to 22 months of age in males and females respectively while tibia showed no change. However, E in ulna increased from 7.0(0.8) GPa to 11.0(1.1) GPa (p = .01) from 6 to 22 months of age in females only. Changes in age and sex-related bone properties were more pronounced in the femur and tibia, while the ulna showed fewer overall differences. Most of the changes were observed in biomechanical compared to architectural properties and female bones are more severely affected by aging. In conclusion, our data demonstrate that care must be taken to describe bone site and sex-specific, rather than making broad generalizations when describing age-related changes on the biomechanical and architectural properties of the skeleton. Elsevier 2020-04-23 /pmc/articles/PMC7215114/ /pubmed/32420415 http://dx.doi.org/10.1016/j.bonr.2020.100266 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Mumtaz, Hammad
Dallas, Mark
Begonia, Mark
Lara-Castillo, Nuria
Scott, JoAnna M.
Johnson, Mark L.
Ganesh, Thiagarajan
Age-related and sex-specific effects on architectural properties and biomechanical response of the C57BL/6N mouse femur, tibia and ulna
title Age-related and sex-specific effects on architectural properties and biomechanical response of the C57BL/6N mouse femur, tibia and ulna
title_full Age-related and sex-specific effects on architectural properties and biomechanical response of the C57BL/6N mouse femur, tibia and ulna
title_fullStr Age-related and sex-specific effects on architectural properties and biomechanical response of the C57BL/6N mouse femur, tibia and ulna
title_full_unstemmed Age-related and sex-specific effects on architectural properties and biomechanical response of the C57BL/6N mouse femur, tibia and ulna
title_short Age-related and sex-specific effects on architectural properties and biomechanical response of the C57BL/6N mouse femur, tibia and ulna
title_sort age-related and sex-specific effects on architectural properties and biomechanical response of the c57bl/6n mouse femur, tibia and ulna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215114/
https://www.ncbi.nlm.nih.gov/pubmed/32420415
http://dx.doi.org/10.1016/j.bonr.2020.100266
work_keys_str_mv AT mumtazhammad agerelatedandsexspecificeffectsonarchitecturalpropertiesandbiomechanicalresponseofthec57bl6nmousefemurtibiaandulna
AT dallasmark agerelatedandsexspecificeffectsonarchitecturalpropertiesandbiomechanicalresponseofthec57bl6nmousefemurtibiaandulna
AT begoniamark agerelatedandsexspecificeffectsonarchitecturalpropertiesandbiomechanicalresponseofthec57bl6nmousefemurtibiaandulna
AT laracastillonuria agerelatedandsexspecificeffectsonarchitecturalpropertiesandbiomechanicalresponseofthec57bl6nmousefemurtibiaandulna
AT scottjoannam agerelatedandsexspecificeffectsonarchitecturalpropertiesandbiomechanicalresponseofthec57bl6nmousefemurtibiaandulna
AT johnsonmarkl agerelatedandsexspecificeffectsonarchitecturalpropertiesandbiomechanicalresponseofthec57bl6nmousefemurtibiaandulna
AT ganeshthiagarajan agerelatedandsexspecificeffectsonarchitecturalpropertiesandbiomechanicalresponseofthec57bl6nmousefemurtibiaandulna