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Acute and Chronic Dopaminergic Depletion Differently Affect Motor Thalamic Function

The motor thalamus (MTh) plays a crucial role in the basal ganglia (BG)-cortical loop in motor information codification. Despite this, there is limited evidence of MTh functionality in normal and Parkinsonian conditions. To shed light on the functional properties of the MTh, we examined the effects...

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Autores principales: Di Giovanni, Giuseppe, Grandi, Laura Clara, Fedele, Ernesto, Orban, Gergely, Salvadè, Agnese, Song, Wei, Cuboni, Eleonora, Stefani, Alessandro, Kaelin-Lang, Alain, Galati, Salvatore
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215393/
https://www.ncbi.nlm.nih.gov/pubmed/32326424
http://dx.doi.org/10.3390/ijms21082734
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author Di Giovanni, Giuseppe
Grandi, Laura Clara
Fedele, Ernesto
Orban, Gergely
Salvadè, Agnese
Song, Wei
Cuboni, Eleonora
Stefani, Alessandro
Kaelin-Lang, Alain
Galati, Salvatore
author_facet Di Giovanni, Giuseppe
Grandi, Laura Clara
Fedele, Ernesto
Orban, Gergely
Salvadè, Agnese
Song, Wei
Cuboni, Eleonora
Stefani, Alessandro
Kaelin-Lang, Alain
Galati, Salvatore
author_sort Di Giovanni, Giuseppe
collection PubMed
description The motor thalamus (MTh) plays a crucial role in the basal ganglia (BG)-cortical loop in motor information codification. Despite this, there is limited evidence of MTh functionality in normal and Parkinsonian conditions. To shed light on the functional properties of the MTh, we examined the effects of acute and chronic dopamine (DA) depletion on the neuronal firing of MTh neurons, cortical/MTh interplay and MTh extracellular concentrations of glutamate (GLU) and gamma-aminobutyric acid (GABA) in two states of DA depletion: acute depletion induced by the tetrodotoxin (TTX) and chronic denervation obtained by 6-hydroxydopamine (6-OHDA), both infused into the medial forebrain bundle (MFB) in anesthetized rats. The acute TTX DA depletion caused a clear-cut reduction in MTh neuronal activity without changes in burst content, whereas the chronic 6-OHDA depletion did not modify the firing rate but increased the burst firing. The phase correlation analysis underscored that the 6-OHDA chronic DA depletion affected the MTh-cortical activity coupling compared to the acute TTX-induced DA depletion state. The TTX acute DA depletion caused a clear-cut increase of the MTh GABA concentration and no change of GLU levels. On the other hand, the 6-OHDA-induced chronic DA depletion led to a significant reduction of local GABA and an increase of GLU levels in the MTh. These data show that MTh is affected by DA depletion and support the hypothesis that a rebalancing of MTh in the chronic condition counterbalances the profound alteration arising after acute DA depletion state.
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spelling pubmed-72153932020-05-18 Acute and Chronic Dopaminergic Depletion Differently Affect Motor Thalamic Function Di Giovanni, Giuseppe Grandi, Laura Clara Fedele, Ernesto Orban, Gergely Salvadè, Agnese Song, Wei Cuboni, Eleonora Stefani, Alessandro Kaelin-Lang, Alain Galati, Salvatore Int J Mol Sci Article The motor thalamus (MTh) plays a crucial role in the basal ganglia (BG)-cortical loop in motor information codification. Despite this, there is limited evidence of MTh functionality in normal and Parkinsonian conditions. To shed light on the functional properties of the MTh, we examined the effects of acute and chronic dopamine (DA) depletion on the neuronal firing of MTh neurons, cortical/MTh interplay and MTh extracellular concentrations of glutamate (GLU) and gamma-aminobutyric acid (GABA) in two states of DA depletion: acute depletion induced by the tetrodotoxin (TTX) and chronic denervation obtained by 6-hydroxydopamine (6-OHDA), both infused into the medial forebrain bundle (MFB) in anesthetized rats. The acute TTX DA depletion caused a clear-cut reduction in MTh neuronal activity without changes in burst content, whereas the chronic 6-OHDA depletion did not modify the firing rate but increased the burst firing. The phase correlation analysis underscored that the 6-OHDA chronic DA depletion affected the MTh-cortical activity coupling compared to the acute TTX-induced DA depletion state. The TTX acute DA depletion caused a clear-cut increase of the MTh GABA concentration and no change of GLU levels. On the other hand, the 6-OHDA-induced chronic DA depletion led to a significant reduction of local GABA and an increase of GLU levels in the MTh. These data show that MTh is affected by DA depletion and support the hypothesis that a rebalancing of MTh in the chronic condition counterbalances the profound alteration arising after acute DA depletion state. MDPI 2020-04-15 /pmc/articles/PMC7215393/ /pubmed/32326424 http://dx.doi.org/10.3390/ijms21082734 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Di Giovanni, Giuseppe
Grandi, Laura Clara
Fedele, Ernesto
Orban, Gergely
Salvadè, Agnese
Song, Wei
Cuboni, Eleonora
Stefani, Alessandro
Kaelin-Lang, Alain
Galati, Salvatore
Acute and Chronic Dopaminergic Depletion Differently Affect Motor Thalamic Function
title Acute and Chronic Dopaminergic Depletion Differently Affect Motor Thalamic Function
title_full Acute and Chronic Dopaminergic Depletion Differently Affect Motor Thalamic Function
title_fullStr Acute and Chronic Dopaminergic Depletion Differently Affect Motor Thalamic Function
title_full_unstemmed Acute and Chronic Dopaminergic Depletion Differently Affect Motor Thalamic Function
title_short Acute and Chronic Dopaminergic Depletion Differently Affect Motor Thalamic Function
title_sort acute and chronic dopaminergic depletion differently affect motor thalamic function
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215393/
https://www.ncbi.nlm.nih.gov/pubmed/32326424
http://dx.doi.org/10.3390/ijms21082734
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