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Modified DNA Aptamers for C-Reactive Protein and Lactate Dehydrogenase-5 with Sub-Nanomolar Affinities

Human C-reactive protein (CRP) and lactate dehydrogenase are important markers in clinical laboratory testing—the former is used to detect in vivo inflammation, and the latter is used to detect cell necrosis and tissue destruction. We developed aptamers that bind to human CRP and human lactate dehyd...

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Autores principales: Minagawa, Hirotaka, Kataoka, Yuka, Fujita, Hiroto, Kuwahara, Masayasu, Horii, Katsunori, Shiratori, Ikuo, Waga, Iwao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215426/
https://www.ncbi.nlm.nih.gov/pubmed/32294882
http://dx.doi.org/10.3390/ijms21082683
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author Minagawa, Hirotaka
Kataoka, Yuka
Fujita, Hiroto
Kuwahara, Masayasu
Horii, Katsunori
Shiratori, Ikuo
Waga, Iwao
author_facet Minagawa, Hirotaka
Kataoka, Yuka
Fujita, Hiroto
Kuwahara, Masayasu
Horii, Katsunori
Shiratori, Ikuo
Waga, Iwao
author_sort Minagawa, Hirotaka
collection PubMed
description Human C-reactive protein (CRP) and lactate dehydrogenase are important markers in clinical laboratory testing—the former is used to detect in vivo inflammation, and the latter is used to detect cell necrosis and tissue destruction. We developed aptamers that bind to human CRP and human lactate dehydrogenase-5 (LDH-5) with high affinities (dissociation constants of 6.2 pM and 235 pM, respectively), applying the systematic evolution of ligands by exponential enrichment (SELEX) method, and by using a modified DNA library containing the following base-appended base modifications: analog adenine derivative at the fifth position of uracil (U(ad)), analog guanine derivative at the fifth position of uracil (U(gu)), and analog adenine derivative at the seventh position of adenine (A(ad)). A potential application of these aptamers as sensor elements includes high-sensitivity target detection in point-of-care testing.
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spelling pubmed-72154262020-05-18 Modified DNA Aptamers for C-Reactive Protein and Lactate Dehydrogenase-5 with Sub-Nanomolar Affinities Minagawa, Hirotaka Kataoka, Yuka Fujita, Hiroto Kuwahara, Masayasu Horii, Katsunori Shiratori, Ikuo Waga, Iwao Int J Mol Sci Article Human C-reactive protein (CRP) and lactate dehydrogenase are important markers in clinical laboratory testing—the former is used to detect in vivo inflammation, and the latter is used to detect cell necrosis and tissue destruction. We developed aptamers that bind to human CRP and human lactate dehydrogenase-5 (LDH-5) with high affinities (dissociation constants of 6.2 pM and 235 pM, respectively), applying the systematic evolution of ligands by exponential enrichment (SELEX) method, and by using a modified DNA library containing the following base-appended base modifications: analog adenine derivative at the fifth position of uracil (U(ad)), analog guanine derivative at the fifth position of uracil (U(gu)), and analog adenine derivative at the seventh position of adenine (A(ad)). A potential application of these aptamers as sensor elements includes high-sensitivity target detection in point-of-care testing. MDPI 2020-04-13 /pmc/articles/PMC7215426/ /pubmed/32294882 http://dx.doi.org/10.3390/ijms21082683 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Minagawa, Hirotaka
Kataoka, Yuka
Fujita, Hiroto
Kuwahara, Masayasu
Horii, Katsunori
Shiratori, Ikuo
Waga, Iwao
Modified DNA Aptamers for C-Reactive Protein and Lactate Dehydrogenase-5 with Sub-Nanomolar Affinities
title Modified DNA Aptamers for C-Reactive Protein and Lactate Dehydrogenase-5 with Sub-Nanomolar Affinities
title_full Modified DNA Aptamers for C-Reactive Protein and Lactate Dehydrogenase-5 with Sub-Nanomolar Affinities
title_fullStr Modified DNA Aptamers for C-Reactive Protein and Lactate Dehydrogenase-5 with Sub-Nanomolar Affinities
title_full_unstemmed Modified DNA Aptamers for C-Reactive Protein and Lactate Dehydrogenase-5 with Sub-Nanomolar Affinities
title_short Modified DNA Aptamers for C-Reactive Protein and Lactate Dehydrogenase-5 with Sub-Nanomolar Affinities
title_sort modified dna aptamers for c-reactive protein and lactate dehydrogenase-5 with sub-nanomolar affinities
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215426/
https://www.ncbi.nlm.nih.gov/pubmed/32294882
http://dx.doi.org/10.3390/ijms21082683
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