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The Effects of Sulglycotide on the Adhesion and the Inflammation of Helicobacter Pylori

Helicobacter pylori (H. pylori) is a primary etiologic factor in gastric diseases. Sulglycotide is a glycopeptide derived from pig duodenal mucin. Esterification of its carbohydrate chains with sulfate groups creates a potent gastroprotective agent used to treat various gastric diseases. We investig...

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Autores principales: Yang, Ji Yeong, Kim, Pumsoo, Jeong, Seok-Hoo, Lee, Seong Woong, Myung, Yu Sik, Baeg, Myong Ki, Kim, Jong-Bae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215434/
https://www.ncbi.nlm.nih.gov/pubmed/32340212
http://dx.doi.org/10.3390/ijerph17082918
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author Yang, Ji Yeong
Kim, Pumsoo
Jeong, Seok-Hoo
Lee, Seong Woong
Myung, Yu Sik
Baeg, Myong Ki
Kim, Jong-Bae
author_facet Yang, Ji Yeong
Kim, Pumsoo
Jeong, Seok-Hoo
Lee, Seong Woong
Myung, Yu Sik
Baeg, Myong Ki
Kim, Jong-Bae
author_sort Yang, Ji Yeong
collection PubMed
description Helicobacter pylori (H. pylori) is a primary etiologic factor in gastric diseases. Sulglycotide is a glycopeptide derived from pig duodenal mucin. Esterification of its carbohydrate chains with sulfate groups creates a potent gastroprotective agent used to treat various gastric diseases. We investigated the inhibitory effects of sulglycotide on adhesion and inflammation after H. pylori infection in human gastric adenocarcinoma cells (AGS cells). H. pylori reference strain 60190 (ATCC 49503) was cultured on Brucella agar supplemented with 10% bovine serum. Sulgylcotide-mediated growth inhibition of H. pylori was evaluated using the broth dilution method. Inhibition of H. pylori adhesion to AGS cells by sulglycotide was assessed using a urease assay. Effects of sulglycotide on the translocation of virulence factors was measured using western blot to detect cytotoxin-associated protein A (CagA) and vacuolating cytotoxin A (VacA) proteins. Inhibition of IL-8 secretion was measured using enzyme-linked immunosorbent assay (ELISA) to determine the effects of sulglycotide on inflammation. Sulglycotide did not inhibit the growth of H. pylori, however, after six and 12 hours of infection on AGS cells, H. pylori adhesion was significantly inhibited by approximately 60% by various concentrations of sulglycotide. Sulglycotide decreased H. pylori virulence factor (CagA and VacA) translocation to AGS cells and inhibited IL-8 secretion. Sulglycotide inhibited H. pylori adhesion and inflammation after infection of AGS cells in vitro. These results support the use of sulglycotide to treat H. pylori infections.
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spelling pubmed-72154342020-05-18 The Effects of Sulglycotide on the Adhesion and the Inflammation of Helicobacter Pylori Yang, Ji Yeong Kim, Pumsoo Jeong, Seok-Hoo Lee, Seong Woong Myung, Yu Sik Baeg, Myong Ki Kim, Jong-Bae Int J Environ Res Public Health Article Helicobacter pylori (H. pylori) is a primary etiologic factor in gastric diseases. Sulglycotide is a glycopeptide derived from pig duodenal mucin. Esterification of its carbohydrate chains with sulfate groups creates a potent gastroprotective agent used to treat various gastric diseases. We investigated the inhibitory effects of sulglycotide on adhesion and inflammation after H. pylori infection in human gastric adenocarcinoma cells (AGS cells). H. pylori reference strain 60190 (ATCC 49503) was cultured on Brucella agar supplemented with 10% bovine serum. Sulgylcotide-mediated growth inhibition of H. pylori was evaluated using the broth dilution method. Inhibition of H. pylori adhesion to AGS cells by sulglycotide was assessed using a urease assay. Effects of sulglycotide on the translocation of virulence factors was measured using western blot to detect cytotoxin-associated protein A (CagA) and vacuolating cytotoxin A (VacA) proteins. Inhibition of IL-8 secretion was measured using enzyme-linked immunosorbent assay (ELISA) to determine the effects of sulglycotide on inflammation. Sulglycotide did not inhibit the growth of H. pylori, however, after six and 12 hours of infection on AGS cells, H. pylori adhesion was significantly inhibited by approximately 60% by various concentrations of sulglycotide. Sulglycotide decreased H. pylori virulence factor (CagA and VacA) translocation to AGS cells and inhibited IL-8 secretion. Sulglycotide inhibited H. pylori adhesion and inflammation after infection of AGS cells in vitro. These results support the use of sulglycotide to treat H. pylori infections. MDPI 2020-04-23 2020-04 /pmc/articles/PMC7215434/ /pubmed/32340212 http://dx.doi.org/10.3390/ijerph17082918 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Ji Yeong
Kim, Pumsoo
Jeong, Seok-Hoo
Lee, Seong Woong
Myung, Yu Sik
Baeg, Myong Ki
Kim, Jong-Bae
The Effects of Sulglycotide on the Adhesion and the Inflammation of Helicobacter Pylori
title The Effects of Sulglycotide on the Adhesion and the Inflammation of Helicobacter Pylori
title_full The Effects of Sulglycotide on the Adhesion and the Inflammation of Helicobacter Pylori
title_fullStr The Effects of Sulglycotide on the Adhesion and the Inflammation of Helicobacter Pylori
title_full_unstemmed The Effects of Sulglycotide on the Adhesion and the Inflammation of Helicobacter Pylori
title_short The Effects of Sulglycotide on the Adhesion and the Inflammation of Helicobacter Pylori
title_sort effects of sulglycotide on the adhesion and the inflammation of helicobacter pylori
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215434/
https://www.ncbi.nlm.nih.gov/pubmed/32340212
http://dx.doi.org/10.3390/ijerph17082918
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