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HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution
Using Raman microscopy, we investigated epithelial cervical cells collected from 96 women with squamous cell carcinoma (SCC) or belonging to groups I, IIa, IIID-1 and IIID-2 according to Munich III classification (IIID-1 and IIID-2 corresponding to Bethesda LSIL and HSIL groups, respectively). All w...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215571/ https://www.ncbi.nlm.nih.gov/pubmed/32290479 http://dx.doi.org/10.3390/ijms21082667 |
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author | Sitarz, Katarzyna Czamara, Krzysztof Bialecka, Joanna Klimek, Malgorzata Zawilinska, Barbara Szostek, Slawa Kaczor, Agnieszka |
author_facet | Sitarz, Katarzyna Czamara, Krzysztof Bialecka, Joanna Klimek, Malgorzata Zawilinska, Barbara Szostek, Slawa Kaczor, Agnieszka |
author_sort | Sitarz, Katarzyna |
collection | PubMed |
description | Using Raman microscopy, we investigated epithelial cervical cells collected from 96 women with squamous cell carcinoma (SCC) or belonging to groups I, IIa, IIID-1 and IIID-2 according to Munich III classification (IIID-1 and IIID-2 corresponding to Bethesda LSIL and HSIL groups, respectively). All women were tested for human papillomavirus (HPV) infection using PCR. Subcellular resolution of Raman microscopy enabled to understand phenotypic differences in a heterogeneous population of cervical cells in the following groups: I/HPV(−), IIa/HPV(−), IIa/HPV(−), LSIL/HPV(−), LSIL/HPV(+), HSIL/HPV(−), HSIL/HPV(+) and cancer cells (SCC/HPV(+)). We showed for the first time that the glycogen content in the cytoplasm decreased with the nucleus size of cervical cells in all studied groups apart from the cancer group. For the subpopulation of large-nucleus cells HPV infection resulted in considerable glycogen depletion compared to HPV negative cells in IIa, LSIL (for both statistical significance, ca. 45%) and HSIL (trend, 37%) groups. We hypothesize that accelerated glycogenolysis in large-nucleus cells may be associated with the increased protein metabolism for HPV positive cells. Our work underlines unique capabilities of Raman microscopy in single cell studies and demonstrate potential of Raman-based methods in HPV diagnostics. |
format | Online Article Text |
id | pubmed-7215571 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72155712020-05-22 HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution Sitarz, Katarzyna Czamara, Krzysztof Bialecka, Joanna Klimek, Malgorzata Zawilinska, Barbara Szostek, Slawa Kaczor, Agnieszka Int J Mol Sci Article Using Raman microscopy, we investigated epithelial cervical cells collected from 96 women with squamous cell carcinoma (SCC) or belonging to groups I, IIa, IIID-1 and IIID-2 according to Munich III classification (IIID-1 and IIID-2 corresponding to Bethesda LSIL and HSIL groups, respectively). All women were tested for human papillomavirus (HPV) infection using PCR. Subcellular resolution of Raman microscopy enabled to understand phenotypic differences in a heterogeneous population of cervical cells in the following groups: I/HPV(−), IIa/HPV(−), IIa/HPV(−), LSIL/HPV(−), LSIL/HPV(+), HSIL/HPV(−), HSIL/HPV(+) and cancer cells (SCC/HPV(+)). We showed for the first time that the glycogen content in the cytoplasm decreased with the nucleus size of cervical cells in all studied groups apart from the cancer group. For the subpopulation of large-nucleus cells HPV infection resulted in considerable glycogen depletion compared to HPV negative cells in IIa, LSIL (for both statistical significance, ca. 45%) and HSIL (trend, 37%) groups. We hypothesize that accelerated glycogenolysis in large-nucleus cells may be associated with the increased protein metabolism for HPV positive cells. Our work underlines unique capabilities of Raman microscopy in single cell studies and demonstrate potential of Raman-based methods in HPV diagnostics. MDPI 2020-04-11 /pmc/articles/PMC7215571/ /pubmed/32290479 http://dx.doi.org/10.3390/ijms21082667 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sitarz, Katarzyna Czamara, Krzysztof Bialecka, Joanna Klimek, Malgorzata Zawilinska, Barbara Szostek, Slawa Kaczor, Agnieszka HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution |
title | HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution |
title_full | HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution |
title_fullStr | HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution |
title_full_unstemmed | HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution |
title_short | HPV Infection Significantly Accelerates Glycogen Metabolism in Cervical Cells with Large Nuclei: Raman Microscopic Study with Subcellular Resolution |
title_sort | hpv infection significantly accelerates glycogen metabolism in cervical cells with large nuclei: raman microscopic study with subcellular resolution |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215571/ https://www.ncbi.nlm.nih.gov/pubmed/32290479 http://dx.doi.org/10.3390/ijms21082667 |
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