Cargando…
Competitive Growth of Sulfate-Reducing Bacteria with Bioleaching Acidophiles for Bioremediation of Heap Bioleaching Residue
Mining waste rocks containing sulfide minerals naturally provide the habitat for iron- and sulfur-oxidizing microbes, and they accelerate the generation of acid mine drainage (AMD) by promoting the oxidation of sulfide minerals. Sulfate-reducing bacteria (SRB) are sometimes employed to treat the AMD...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215573/ https://www.ncbi.nlm.nih.gov/pubmed/32326522 http://dx.doi.org/10.3390/ijerph17082715 |
_version_ | 1783532219506098176 |
---|---|
author | Phyo, Aung Kyaw Jia, Yan Tan, Qiaoyi Sun, Heyun Liu, Yunfeng Dong, Bingxu Ruan, Renman |
author_facet | Phyo, Aung Kyaw Jia, Yan Tan, Qiaoyi Sun, Heyun Liu, Yunfeng Dong, Bingxu Ruan, Renman |
author_sort | Phyo, Aung Kyaw |
collection | PubMed |
description | Mining waste rocks containing sulfide minerals naturally provide the habitat for iron- and sulfur-oxidizing microbes, and they accelerate the generation of acid mine drainage (AMD) by promoting the oxidation of sulfide minerals. Sulfate-reducing bacteria (SRB) are sometimes employed to treat the AMD solution by microbial-induced metal sulfide precipitation. It was attempted for the first time to grow SRB directly in the pyritic heap bioleaching residue to compete with the local iron- and sulfur-oxidizing microbes. The acidic SRB and iron-reducing microbes were cultured at pH 2.0 and 3.0. After it was applied to the acidic heap bioleaching residue, it showed that the elevated pH and the organic matter was important for them to compete with the local bioleaching acidophiles. The incubation with the addition of organic matter promoted the growth of SRB and iron-reducing microbes to inhibit the iron- and sulfur-oxidizing microbes, especially organic matter together with some lime. Under the growth of the SRB and iron-reducing microbes, pH increased from acidic to nearly neutral, the Eh also decreased, and the metal, precipitated together with the microbial-generated sulfide, resulted in very low Cu in the residue pore solution. These results prove the inhibition of acid mine drainage directly in situ of the pyritic waste rocks by the promotion of the growth of SRB and iron-reducing microbes to compete with local iron and sulfur-oxidizing microbes, which can be used for the source control of AMD from the sulfidic waste rocks and the final remediation. |
format | Online Article Text |
id | pubmed-7215573 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72155732020-05-22 Competitive Growth of Sulfate-Reducing Bacteria with Bioleaching Acidophiles for Bioremediation of Heap Bioleaching Residue Phyo, Aung Kyaw Jia, Yan Tan, Qiaoyi Sun, Heyun Liu, Yunfeng Dong, Bingxu Ruan, Renman Int J Environ Res Public Health Article Mining waste rocks containing sulfide minerals naturally provide the habitat for iron- and sulfur-oxidizing microbes, and they accelerate the generation of acid mine drainage (AMD) by promoting the oxidation of sulfide minerals. Sulfate-reducing bacteria (SRB) are sometimes employed to treat the AMD solution by microbial-induced metal sulfide precipitation. It was attempted for the first time to grow SRB directly in the pyritic heap bioleaching residue to compete with the local iron- and sulfur-oxidizing microbes. The acidic SRB and iron-reducing microbes were cultured at pH 2.0 and 3.0. After it was applied to the acidic heap bioleaching residue, it showed that the elevated pH and the organic matter was important for them to compete with the local bioleaching acidophiles. The incubation with the addition of organic matter promoted the growth of SRB and iron-reducing microbes to inhibit the iron- and sulfur-oxidizing microbes, especially organic matter together with some lime. Under the growth of the SRB and iron-reducing microbes, pH increased from acidic to nearly neutral, the Eh also decreased, and the metal, precipitated together with the microbial-generated sulfide, resulted in very low Cu in the residue pore solution. These results prove the inhibition of acid mine drainage directly in situ of the pyritic waste rocks by the promotion of the growth of SRB and iron-reducing microbes to compete with local iron and sulfur-oxidizing microbes, which can be used for the source control of AMD from the sulfidic waste rocks and the final remediation. MDPI 2020-04-15 2020-04 /pmc/articles/PMC7215573/ /pubmed/32326522 http://dx.doi.org/10.3390/ijerph17082715 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Phyo, Aung Kyaw Jia, Yan Tan, Qiaoyi Sun, Heyun Liu, Yunfeng Dong, Bingxu Ruan, Renman Competitive Growth of Sulfate-Reducing Bacteria with Bioleaching Acidophiles for Bioremediation of Heap Bioleaching Residue |
title | Competitive Growth of Sulfate-Reducing Bacteria with Bioleaching Acidophiles for Bioremediation of Heap Bioleaching Residue |
title_full | Competitive Growth of Sulfate-Reducing Bacteria with Bioleaching Acidophiles for Bioremediation of Heap Bioleaching Residue |
title_fullStr | Competitive Growth of Sulfate-Reducing Bacteria with Bioleaching Acidophiles for Bioremediation of Heap Bioleaching Residue |
title_full_unstemmed | Competitive Growth of Sulfate-Reducing Bacteria with Bioleaching Acidophiles for Bioremediation of Heap Bioleaching Residue |
title_short | Competitive Growth of Sulfate-Reducing Bacteria with Bioleaching Acidophiles for Bioremediation of Heap Bioleaching Residue |
title_sort | competitive growth of sulfate-reducing bacteria with bioleaching acidophiles for bioremediation of heap bioleaching residue |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215573/ https://www.ncbi.nlm.nih.gov/pubmed/32326522 http://dx.doi.org/10.3390/ijerph17082715 |
work_keys_str_mv | AT phyoaungkyaw competitivegrowthofsulfatereducingbacteriawithbioleachingacidophilesforbioremediationofheapbioleachingresidue AT jiayan competitivegrowthofsulfatereducingbacteriawithbioleachingacidophilesforbioremediationofheapbioleachingresidue AT tanqiaoyi competitivegrowthofsulfatereducingbacteriawithbioleachingacidophilesforbioremediationofheapbioleachingresidue AT sunheyun competitivegrowthofsulfatereducingbacteriawithbioleachingacidophilesforbioremediationofheapbioleachingresidue AT liuyunfeng competitivegrowthofsulfatereducingbacteriawithbioleachingacidophilesforbioremediationofheapbioleachingresidue AT dongbingxu competitivegrowthofsulfatereducingbacteriawithbioleachingacidophilesforbioremediationofheapbioleachingresidue AT ruanrenman competitivegrowthofsulfatereducingbacteriawithbioleachingacidophilesforbioremediationofheapbioleachingresidue |