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The Importance of Porins and β-Lactamase in Outer Membrane Vesicles on the Hydrolysis of β-Lactam Antibiotics

Gram-negative bacteria have an outer membrane inhibiting the entry of antibiotics. Porins, found within the outer membrane, are involved in regulating the permeability of β-lactam antibiotics. β-lactamases are enzymes that are able to inactivate the antibacterial properties of β-lactam antibiotics....

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Autores principales: Kim, Si Won, Lee, Jung Seok, Park, Seong Bin, Lee, Ae Rin, Jung, Jae Wook, Chun, Jin Hong, Lazarte, Jassy Mary S., Kim, Jaesung, Seo, Jong-Su, Kim, Jong-Hwan, Song, Jong-Wook, Ha, Min Woo, Thompson, Kim D., Lee, Chang-Ro, Jung, Myunghwan, Jung, Tae Sung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215730/
https://www.ncbi.nlm.nih.gov/pubmed/32316670
http://dx.doi.org/10.3390/ijms21082822
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author Kim, Si Won
Lee, Jung Seok
Park, Seong Bin
Lee, Ae Rin
Jung, Jae Wook
Chun, Jin Hong
Lazarte, Jassy Mary S.
Kim, Jaesung
Seo, Jong-Su
Kim, Jong-Hwan
Song, Jong-Wook
Ha, Min Woo
Thompson, Kim D.
Lee, Chang-Ro
Jung, Myunghwan
Jung, Tae Sung
author_facet Kim, Si Won
Lee, Jung Seok
Park, Seong Bin
Lee, Ae Rin
Jung, Jae Wook
Chun, Jin Hong
Lazarte, Jassy Mary S.
Kim, Jaesung
Seo, Jong-Su
Kim, Jong-Hwan
Song, Jong-Wook
Ha, Min Woo
Thompson, Kim D.
Lee, Chang-Ro
Jung, Myunghwan
Jung, Tae Sung
author_sort Kim, Si Won
collection PubMed
description Gram-negative bacteria have an outer membrane inhibiting the entry of antibiotics. Porins, found within the outer membrane, are involved in regulating the permeability of β-lactam antibiotics. β-lactamases are enzymes that are able to inactivate the antibacterial properties of β-lactam antibiotics. Interestingly, porins and β-lactamase are found in outer membrane vesicles (OMVs) of β-lactam-resistant Escherichia coli and may be involved in the survival of susceptible strains of E. coli in the presence of antibiotics, through the hydrolysis of the β-lactam antibiotic. In this study, OMVs isolated from β-lactam-resistant E. coli and from mutants, lacking porin or β-lactamase, were evaluated to establish if the porins or β-lactamase in OMVs were involved in the degradation of β-lactam antibiotics. OMVs isolated from E. coli deficient in β-lactamase did not show any degradation ability against β-lactam antibiotics, while OMVs lacking OmpC or OmpF showed significantly lower levels of hydrolyzing activity than OMVs from parent E. coli. These data reveal an important role of OMVs in bacterial defense mechanisms demonstrating that the OmpC and OmpF proteins allow permeation of β-lactam antibiotics into the lumen of OMVs, and antibiotics that enter the OMVs can be degraded by β-lactamase.
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spelling pubmed-72157302020-05-22 The Importance of Porins and β-Lactamase in Outer Membrane Vesicles on the Hydrolysis of β-Lactam Antibiotics Kim, Si Won Lee, Jung Seok Park, Seong Bin Lee, Ae Rin Jung, Jae Wook Chun, Jin Hong Lazarte, Jassy Mary S. Kim, Jaesung Seo, Jong-Su Kim, Jong-Hwan Song, Jong-Wook Ha, Min Woo Thompson, Kim D. Lee, Chang-Ro Jung, Myunghwan Jung, Tae Sung Int J Mol Sci Article Gram-negative bacteria have an outer membrane inhibiting the entry of antibiotics. Porins, found within the outer membrane, are involved in regulating the permeability of β-lactam antibiotics. β-lactamases are enzymes that are able to inactivate the antibacterial properties of β-lactam antibiotics. Interestingly, porins and β-lactamase are found in outer membrane vesicles (OMVs) of β-lactam-resistant Escherichia coli and may be involved in the survival of susceptible strains of E. coli in the presence of antibiotics, through the hydrolysis of the β-lactam antibiotic. In this study, OMVs isolated from β-lactam-resistant E. coli and from mutants, lacking porin or β-lactamase, were evaluated to establish if the porins or β-lactamase in OMVs were involved in the degradation of β-lactam antibiotics. OMVs isolated from E. coli deficient in β-lactamase did not show any degradation ability against β-lactam antibiotics, while OMVs lacking OmpC or OmpF showed significantly lower levels of hydrolyzing activity than OMVs from parent E. coli. These data reveal an important role of OMVs in bacterial defense mechanisms demonstrating that the OmpC and OmpF proteins allow permeation of β-lactam antibiotics into the lumen of OMVs, and antibiotics that enter the OMVs can be degraded by β-lactamase. MDPI 2020-04-17 /pmc/articles/PMC7215730/ /pubmed/32316670 http://dx.doi.org/10.3390/ijms21082822 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Si Won
Lee, Jung Seok
Park, Seong Bin
Lee, Ae Rin
Jung, Jae Wook
Chun, Jin Hong
Lazarte, Jassy Mary S.
Kim, Jaesung
Seo, Jong-Su
Kim, Jong-Hwan
Song, Jong-Wook
Ha, Min Woo
Thompson, Kim D.
Lee, Chang-Ro
Jung, Myunghwan
Jung, Tae Sung
The Importance of Porins and β-Lactamase in Outer Membrane Vesicles on the Hydrolysis of β-Lactam Antibiotics
title The Importance of Porins and β-Lactamase in Outer Membrane Vesicles on the Hydrolysis of β-Lactam Antibiotics
title_full The Importance of Porins and β-Lactamase in Outer Membrane Vesicles on the Hydrolysis of β-Lactam Antibiotics
title_fullStr The Importance of Porins and β-Lactamase in Outer Membrane Vesicles on the Hydrolysis of β-Lactam Antibiotics
title_full_unstemmed The Importance of Porins and β-Lactamase in Outer Membrane Vesicles on the Hydrolysis of β-Lactam Antibiotics
title_short The Importance of Porins and β-Lactamase in Outer Membrane Vesicles on the Hydrolysis of β-Lactam Antibiotics
title_sort importance of porins and β-lactamase in outer membrane vesicles on the hydrolysis of β-lactam antibiotics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215730/
https://www.ncbi.nlm.nih.gov/pubmed/32316670
http://dx.doi.org/10.3390/ijms21082822
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