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3-Hydroxybutyrate Is Active Compound in Flax that Upregulates Genes Involved in DNA Methylation
In mammalian cells, 3-hydroxybutyrate (3-HB) is not only an intermediate metabolite during the oxidation of fatty acids, but also an important signaling molecule. On the other hand, the information about the metabolism or function of this compound in plants is scarce. In our study, we show for the f...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215830/ https://www.ncbi.nlm.nih.gov/pubmed/32326145 http://dx.doi.org/10.3390/ijms21082887 |
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author | Mierziak, Justyna Wojtasik, Wioleta Kulma, Anna Dziadas, Mariusz Kostyn, Kamil Dymińska, Lucyna Hanuza, Jerzy Żuk, Magdalena Szopa, Jan |
author_facet | Mierziak, Justyna Wojtasik, Wioleta Kulma, Anna Dziadas, Mariusz Kostyn, Kamil Dymińska, Lucyna Hanuza, Jerzy Żuk, Magdalena Szopa, Jan |
author_sort | Mierziak, Justyna |
collection | PubMed |
description | In mammalian cells, 3-hydroxybutyrate (3-HB) is not only an intermediate metabolite during the oxidation of fatty acids, but also an important signaling molecule. On the other hand, the information about the metabolism or function of this compound in plants is scarce. In our study, we show for the first time that this compound naturally occurs in flax. The expression of bacterial β-ketothiolase in flax affects expression of endogenous genes of the 3-HB biosynthesis pathway and the compound content. The increase in 3-HB content in transgenic plants or after control plants treatment with 3-HB resulted in upregulation of genes involved in chromatin remodeling. The observation that 3-HB is an endogenous activator of methyltransferase 3 (CMT3), decreased DNA methylation I (DDM1), DEMETER DNA glycosylase (DME), and an inhibitor of sirtuin 1 (SRT1) provides an example of integration of different genes in chromatin remodeling. The changes in chromatin remodeling gene expression concomitant with those involved in phenolics and the lignin biosynthesis pathway suggest potential integration of secondary metabolic status with epigenetic changes. |
format | Online Article Text |
id | pubmed-7215830 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72158302020-05-22 3-Hydroxybutyrate Is Active Compound in Flax that Upregulates Genes Involved in DNA Methylation Mierziak, Justyna Wojtasik, Wioleta Kulma, Anna Dziadas, Mariusz Kostyn, Kamil Dymińska, Lucyna Hanuza, Jerzy Żuk, Magdalena Szopa, Jan Int J Mol Sci Article In mammalian cells, 3-hydroxybutyrate (3-HB) is not only an intermediate metabolite during the oxidation of fatty acids, but also an important signaling molecule. On the other hand, the information about the metabolism or function of this compound in plants is scarce. In our study, we show for the first time that this compound naturally occurs in flax. The expression of bacterial β-ketothiolase in flax affects expression of endogenous genes of the 3-HB biosynthesis pathway and the compound content. The increase in 3-HB content in transgenic plants or after control plants treatment with 3-HB resulted in upregulation of genes involved in chromatin remodeling. The observation that 3-HB is an endogenous activator of methyltransferase 3 (CMT3), decreased DNA methylation I (DDM1), DEMETER DNA glycosylase (DME), and an inhibitor of sirtuin 1 (SRT1) provides an example of integration of different genes in chromatin remodeling. The changes in chromatin remodeling gene expression concomitant with those involved in phenolics and the lignin biosynthesis pathway suggest potential integration of secondary metabolic status with epigenetic changes. MDPI 2020-04-21 /pmc/articles/PMC7215830/ /pubmed/32326145 http://dx.doi.org/10.3390/ijms21082887 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mierziak, Justyna Wojtasik, Wioleta Kulma, Anna Dziadas, Mariusz Kostyn, Kamil Dymińska, Lucyna Hanuza, Jerzy Żuk, Magdalena Szopa, Jan 3-Hydroxybutyrate Is Active Compound in Flax that Upregulates Genes Involved in DNA Methylation |
title | 3-Hydroxybutyrate Is Active Compound in Flax that Upregulates Genes Involved in DNA Methylation |
title_full | 3-Hydroxybutyrate Is Active Compound in Flax that Upregulates Genes Involved in DNA Methylation |
title_fullStr | 3-Hydroxybutyrate Is Active Compound in Flax that Upregulates Genes Involved in DNA Methylation |
title_full_unstemmed | 3-Hydroxybutyrate Is Active Compound in Flax that Upregulates Genes Involved in DNA Methylation |
title_short | 3-Hydroxybutyrate Is Active Compound in Flax that Upregulates Genes Involved in DNA Methylation |
title_sort | 3-hydroxybutyrate is active compound in flax that upregulates genes involved in dna methylation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215830/ https://www.ncbi.nlm.nih.gov/pubmed/32326145 http://dx.doi.org/10.3390/ijms21082887 |
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