RT-qPCR Testing of SARS-CoV-2: A Primer

Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely used method is quantitative fluorescence-based re...

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Detalles Bibliográficos
Autores principales: Bustin, Stephen A., Nolan, Tania
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Commentary
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215906/
https://www.ncbi.nlm.nih.gov/pubmed/32344568
http://dx.doi.org/10.3390/ijms21083004
Descripción
Sumario:Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely used method is quantitative fluorescence-based reverse transcription polymerase chain reaction (RT-qPCR). Despite its ubiquity, there is a significant amount of uncertainty about how this test works, potential throughput and reliability. This has resulted in widespread misrepresentation of the problems faced using this test during the current COVID-19 epidemic. This primer provides simple, straightforward and impartial information about RT-qPCR.

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