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Effects of Cryopreservation on Cell Metabolic Activity and Function of Biofabricated Structures Laden with Osteoblasts

Biofabrication and maturation of bone constructs is a long-term task that requires a high degree of specialization. This specialization falls onto the hierarchy complexity of the bone tissue that limits the transfer of this technology to the clinic. This work studied the effects of the short-term cr...

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Autores principales: Hernández-Tapia, Laura G., Fohlerová, Zdenka, Žídek, Jan, Alvarez-Perez, Marco A., Čelko, Ladislav, Kaiser, Jozef, Montufar, Edgar B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215951/
https://www.ncbi.nlm.nih.gov/pubmed/32331435
http://dx.doi.org/10.3390/ma13081966
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author Hernández-Tapia, Laura G.
Fohlerová, Zdenka
Žídek, Jan
Alvarez-Perez, Marco A.
Čelko, Ladislav
Kaiser, Jozef
Montufar, Edgar B.
author_facet Hernández-Tapia, Laura G.
Fohlerová, Zdenka
Žídek, Jan
Alvarez-Perez, Marco A.
Čelko, Ladislav
Kaiser, Jozef
Montufar, Edgar B.
author_sort Hernández-Tapia, Laura G.
collection PubMed
description Biofabrication and maturation of bone constructs is a long-term task that requires a high degree of specialization. This specialization falls onto the hierarchy complexity of the bone tissue that limits the transfer of this technology to the clinic. This work studied the effects of the short-term cryopreservation on biofabricated osteoblast-containing structures, with the final aim to make them steadily available in biobanks. The biological responses studied include the osteoblast post-thawing metabolic activity and the recovery of the osteoblastic function of 3D-bioprinted osteoblastic structures and beta tricalcium phosphate (β-TCP) scaffolds infiltrated with osteoblasts encapsulated in a hydrogel. The obtained structures were cryopreserved at −80 °C for 7 days using dimethyl sulfoxide (DMSO) as cryoprotectant additive. After thawing the structures were cultured up to 14 days. The results revealed fundamental biological aspects for the successful cryopreservation of osteoblast constructs. In summary, immature osteoblasts take longer to recover than mature osteoblasts. The pre-cryopreservation culture period had an important effect on the metabolic activity and function maintain, faster recovering normal values when cryopreserved after longer-term culture (7 days). The use of β-TCP scaffolds further improved the osteoblast survival after cryopreservation, resulting in similar levels of alkaline phosphatase activity in comparison with the non-preserved structures. These results contribute to the understanding of the biology of cryopreserved osteoblast constructs, approaching biofabrication to the clinical practice.
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spelling pubmed-72159512020-05-22 Effects of Cryopreservation on Cell Metabolic Activity and Function of Biofabricated Structures Laden with Osteoblasts Hernández-Tapia, Laura G. Fohlerová, Zdenka Žídek, Jan Alvarez-Perez, Marco A. Čelko, Ladislav Kaiser, Jozef Montufar, Edgar B. Materials (Basel) Article Biofabrication and maturation of bone constructs is a long-term task that requires a high degree of specialization. This specialization falls onto the hierarchy complexity of the bone tissue that limits the transfer of this technology to the clinic. This work studied the effects of the short-term cryopreservation on biofabricated osteoblast-containing structures, with the final aim to make them steadily available in biobanks. The biological responses studied include the osteoblast post-thawing metabolic activity and the recovery of the osteoblastic function of 3D-bioprinted osteoblastic structures and beta tricalcium phosphate (β-TCP) scaffolds infiltrated with osteoblasts encapsulated in a hydrogel. The obtained structures were cryopreserved at −80 °C for 7 days using dimethyl sulfoxide (DMSO) as cryoprotectant additive. After thawing the structures were cultured up to 14 days. The results revealed fundamental biological aspects for the successful cryopreservation of osteoblast constructs. In summary, immature osteoblasts take longer to recover than mature osteoblasts. The pre-cryopreservation culture period had an important effect on the metabolic activity and function maintain, faster recovering normal values when cryopreserved after longer-term culture (7 days). The use of β-TCP scaffolds further improved the osteoblast survival after cryopreservation, resulting in similar levels of alkaline phosphatase activity in comparison with the non-preserved structures. These results contribute to the understanding of the biology of cryopreserved osteoblast constructs, approaching biofabrication to the clinical practice. MDPI 2020-04-22 /pmc/articles/PMC7215951/ /pubmed/32331435 http://dx.doi.org/10.3390/ma13081966 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hernández-Tapia, Laura G.
Fohlerová, Zdenka
Žídek, Jan
Alvarez-Perez, Marco A.
Čelko, Ladislav
Kaiser, Jozef
Montufar, Edgar B.
Effects of Cryopreservation on Cell Metabolic Activity and Function of Biofabricated Structures Laden with Osteoblasts
title Effects of Cryopreservation on Cell Metabolic Activity and Function of Biofabricated Structures Laden with Osteoblasts
title_full Effects of Cryopreservation on Cell Metabolic Activity and Function of Biofabricated Structures Laden with Osteoblasts
title_fullStr Effects of Cryopreservation on Cell Metabolic Activity and Function of Biofabricated Structures Laden with Osteoblasts
title_full_unstemmed Effects of Cryopreservation on Cell Metabolic Activity and Function of Biofabricated Structures Laden with Osteoblasts
title_short Effects of Cryopreservation on Cell Metabolic Activity and Function of Biofabricated Structures Laden with Osteoblasts
title_sort effects of cryopreservation on cell metabolic activity and function of biofabricated structures laden with osteoblasts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215951/
https://www.ncbi.nlm.nih.gov/pubmed/32331435
http://dx.doi.org/10.3390/ma13081966
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