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Cerato-Platanins from Marine Fungi as Effective Protein Biosurfactants and Bioemulsifiers

Two fungal strains, Aspergillus terreus MUT 271 and Trichoderma harzianum MUT 290, isolated from a Mediterranean marine site chronically pervaded by oil spills, can use crude oil as sole carbon source. Herein, these strains were investigated as producers of biosurfactants, apt to solubilize organic...

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Autores principales: Pitocchi, Rossana, Cicatiello, Paola, Birolo, Leila, Piscitelli, Alessandra, Bovio, Elena, Varese, Giovanna Cristina, Giardina, Paola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215997/
https://www.ncbi.nlm.nih.gov/pubmed/32326352
http://dx.doi.org/10.3390/ijms21082913
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author Pitocchi, Rossana
Cicatiello, Paola
Birolo, Leila
Piscitelli, Alessandra
Bovio, Elena
Varese, Giovanna Cristina
Giardina, Paola
author_facet Pitocchi, Rossana
Cicatiello, Paola
Birolo, Leila
Piscitelli, Alessandra
Bovio, Elena
Varese, Giovanna Cristina
Giardina, Paola
author_sort Pitocchi, Rossana
collection PubMed
description Two fungal strains, Aspergillus terreus MUT 271 and Trichoderma harzianum MUT 290, isolated from a Mediterranean marine site chronically pervaded by oil spills, can use crude oil as sole carbon source. Herein, these strains were investigated as producers of biosurfactants, apt to solubilize organic molecules as a preliminary step to metabolize them. Both fungi secreted low molecular weight proteins identified as cerato-platanins, small, conserved, hydrophobic proteins, included among the fungal surface-active proteins. Both proteins were able to stabilize emulsions, and their capacity was comparable to that of other biosurfactant proteins and to commercially available surfactants. Moreover, the cerato-platanin from T. harzianum was able to lower the surface tension value to a larger extent than the similar protein from A. terreus and other amphiphilic proteins from fungi. Both cerato-platanins were able to make hydrophilic a hydrophobic surface, such as hydrophobins, and to form a stable layer, not removable even after surface washing. To the best of our knowledge, the ability of cerato-platanins to work both as biosurfactant and bioemulsifier is herein demonstrated for the first time.
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spelling pubmed-72159972020-05-22 Cerato-Platanins from Marine Fungi as Effective Protein Biosurfactants and Bioemulsifiers Pitocchi, Rossana Cicatiello, Paola Birolo, Leila Piscitelli, Alessandra Bovio, Elena Varese, Giovanna Cristina Giardina, Paola Int J Mol Sci Article Two fungal strains, Aspergillus terreus MUT 271 and Trichoderma harzianum MUT 290, isolated from a Mediterranean marine site chronically pervaded by oil spills, can use crude oil as sole carbon source. Herein, these strains were investigated as producers of biosurfactants, apt to solubilize organic molecules as a preliminary step to metabolize them. Both fungi secreted low molecular weight proteins identified as cerato-platanins, small, conserved, hydrophobic proteins, included among the fungal surface-active proteins. Both proteins were able to stabilize emulsions, and their capacity was comparable to that of other biosurfactant proteins and to commercially available surfactants. Moreover, the cerato-platanin from T. harzianum was able to lower the surface tension value to a larger extent than the similar protein from A. terreus and other amphiphilic proteins from fungi. Both cerato-platanins were able to make hydrophilic a hydrophobic surface, such as hydrophobins, and to form a stable layer, not removable even after surface washing. To the best of our knowledge, the ability of cerato-platanins to work both as biosurfactant and bioemulsifier is herein demonstrated for the first time. MDPI 2020-04-21 /pmc/articles/PMC7215997/ /pubmed/32326352 http://dx.doi.org/10.3390/ijms21082913 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pitocchi, Rossana
Cicatiello, Paola
Birolo, Leila
Piscitelli, Alessandra
Bovio, Elena
Varese, Giovanna Cristina
Giardina, Paola
Cerato-Platanins from Marine Fungi as Effective Protein Biosurfactants and Bioemulsifiers
title Cerato-Platanins from Marine Fungi as Effective Protein Biosurfactants and Bioemulsifiers
title_full Cerato-Platanins from Marine Fungi as Effective Protein Biosurfactants and Bioemulsifiers
title_fullStr Cerato-Platanins from Marine Fungi as Effective Protein Biosurfactants and Bioemulsifiers
title_full_unstemmed Cerato-Platanins from Marine Fungi as Effective Protein Biosurfactants and Bioemulsifiers
title_short Cerato-Platanins from Marine Fungi as Effective Protein Biosurfactants and Bioemulsifiers
title_sort cerato-platanins from marine fungi as effective protein biosurfactants and bioemulsifiers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215997/
https://www.ncbi.nlm.nih.gov/pubmed/32326352
http://dx.doi.org/10.3390/ijms21082913
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