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Cerato-Platanins from Marine Fungi as Effective Protein Biosurfactants and Bioemulsifiers
Two fungal strains, Aspergillus terreus MUT 271 and Trichoderma harzianum MUT 290, isolated from a Mediterranean marine site chronically pervaded by oil spills, can use crude oil as sole carbon source. Herein, these strains were investigated as producers of biosurfactants, apt to solubilize organic...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215997/ https://www.ncbi.nlm.nih.gov/pubmed/32326352 http://dx.doi.org/10.3390/ijms21082913 |
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author | Pitocchi, Rossana Cicatiello, Paola Birolo, Leila Piscitelli, Alessandra Bovio, Elena Varese, Giovanna Cristina Giardina, Paola |
author_facet | Pitocchi, Rossana Cicatiello, Paola Birolo, Leila Piscitelli, Alessandra Bovio, Elena Varese, Giovanna Cristina Giardina, Paola |
author_sort | Pitocchi, Rossana |
collection | PubMed |
description | Two fungal strains, Aspergillus terreus MUT 271 and Trichoderma harzianum MUT 290, isolated from a Mediterranean marine site chronically pervaded by oil spills, can use crude oil as sole carbon source. Herein, these strains were investigated as producers of biosurfactants, apt to solubilize organic molecules as a preliminary step to metabolize them. Both fungi secreted low molecular weight proteins identified as cerato-platanins, small, conserved, hydrophobic proteins, included among the fungal surface-active proteins. Both proteins were able to stabilize emulsions, and their capacity was comparable to that of other biosurfactant proteins and to commercially available surfactants. Moreover, the cerato-platanin from T. harzianum was able to lower the surface tension value to a larger extent than the similar protein from A. terreus and other amphiphilic proteins from fungi. Both cerato-platanins were able to make hydrophilic a hydrophobic surface, such as hydrophobins, and to form a stable layer, not removable even after surface washing. To the best of our knowledge, the ability of cerato-platanins to work both as biosurfactant and bioemulsifier is herein demonstrated for the first time. |
format | Online Article Text |
id | pubmed-7215997 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72159972020-05-22 Cerato-Platanins from Marine Fungi as Effective Protein Biosurfactants and Bioemulsifiers Pitocchi, Rossana Cicatiello, Paola Birolo, Leila Piscitelli, Alessandra Bovio, Elena Varese, Giovanna Cristina Giardina, Paola Int J Mol Sci Article Two fungal strains, Aspergillus terreus MUT 271 and Trichoderma harzianum MUT 290, isolated from a Mediterranean marine site chronically pervaded by oil spills, can use crude oil as sole carbon source. Herein, these strains were investigated as producers of biosurfactants, apt to solubilize organic molecules as a preliminary step to metabolize them. Both fungi secreted low molecular weight proteins identified as cerato-platanins, small, conserved, hydrophobic proteins, included among the fungal surface-active proteins. Both proteins were able to stabilize emulsions, and their capacity was comparable to that of other biosurfactant proteins and to commercially available surfactants. Moreover, the cerato-platanin from T. harzianum was able to lower the surface tension value to a larger extent than the similar protein from A. terreus and other amphiphilic proteins from fungi. Both cerato-platanins were able to make hydrophilic a hydrophobic surface, such as hydrophobins, and to form a stable layer, not removable even after surface washing. To the best of our knowledge, the ability of cerato-platanins to work both as biosurfactant and bioemulsifier is herein demonstrated for the first time. MDPI 2020-04-21 /pmc/articles/PMC7215997/ /pubmed/32326352 http://dx.doi.org/10.3390/ijms21082913 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pitocchi, Rossana Cicatiello, Paola Birolo, Leila Piscitelli, Alessandra Bovio, Elena Varese, Giovanna Cristina Giardina, Paola Cerato-Platanins from Marine Fungi as Effective Protein Biosurfactants and Bioemulsifiers |
title | Cerato-Platanins from Marine Fungi as Effective Protein Biosurfactants and Bioemulsifiers |
title_full | Cerato-Platanins from Marine Fungi as Effective Protein Biosurfactants and Bioemulsifiers |
title_fullStr | Cerato-Platanins from Marine Fungi as Effective Protein Biosurfactants and Bioemulsifiers |
title_full_unstemmed | Cerato-Platanins from Marine Fungi as Effective Protein Biosurfactants and Bioemulsifiers |
title_short | Cerato-Platanins from Marine Fungi as Effective Protein Biosurfactants and Bioemulsifiers |
title_sort | cerato-platanins from marine fungi as effective protein biosurfactants and bioemulsifiers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215997/ https://www.ncbi.nlm.nih.gov/pubmed/32326352 http://dx.doi.org/10.3390/ijms21082913 |
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