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Chemerin Treatment Inhibits the Growth and Bone Invasion of Breast Cancer Cells

Chemerin is secreted as prochemerin from various cell types and then cleaved into the bioactive isoform by specific proteases. In various cancer types, chemerin exhibits pro- or antitumor effects. In the present study, chemerin treatment significantly inhibited the viability and invasion of breast c...

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Autores principales: Kim, Hyungkeun, Lee, Joo-Hee, Lee, Sun Kyoung, Song, Na-Young, Son, Seung Hwa, Kim, Ki Rim, Chung, Won-Yoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216174/
https://www.ncbi.nlm.nih.gov/pubmed/32325994
http://dx.doi.org/10.3390/ijms21082871
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author Kim, Hyungkeun
Lee, Joo-Hee
Lee, Sun Kyoung
Song, Na-Young
Son, Seung Hwa
Kim, Ki Rim
Chung, Won-Yoon
author_facet Kim, Hyungkeun
Lee, Joo-Hee
Lee, Sun Kyoung
Song, Na-Young
Son, Seung Hwa
Kim, Ki Rim
Chung, Won-Yoon
author_sort Kim, Hyungkeun
collection PubMed
description Chemerin is secreted as prochemerin from various cell types and then cleaved into the bioactive isoform by specific proteases. In various cancer types, chemerin exhibits pro- or antitumor effects. In the present study, chemerin treatment significantly inhibited the viability and invasion of breast cancer cells in the absence or presence of transforming growth factor (TGF)-β and insulin-like growth factor (IGF)-1. The expression levels of E-cadherin and vimentin were reduced in chemerin-treated breast cancer cells. However, chemerin treatment recovered the reduced E-cadherin expression level in breast cancer cells treated with TGF-β or IGF-1. Chemerin treatment inhibited nuclear β-catenin levels in breast cancer cells stimulated with or without TGF-β or IGF-1. In addition, chemerin treatment blocked the increase in the receptor activator of nuclear factor kappa-Β ligand (RANKL)/osteoprotegerin (OPG) ratio in osteoblastic cells exposed to metastatic breast cancer cell-derived conditioned medium. Chemerin treatment inhibited RANKL-induced osteoclast formation and bone resorption by reducing the secretion of matrix metalloproteinase (MMP)-2, MMP-9, and cathepsin K. Intraperitoneal administration of chemerin inhibited tumor growth in MCF-7 breast cancer cell-injected mice and reduced the development of osteolytic lesions resulting from intratibial inoculation of MDA-MB-231 cells. Taken together, chemerin inhibits the growth and invasion of breast cancer cells and prevents bone loss resulting from breast cancer cells by inhibiting finally osteoclast formation and activity.
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spelling pubmed-72161742020-05-22 Chemerin Treatment Inhibits the Growth and Bone Invasion of Breast Cancer Cells Kim, Hyungkeun Lee, Joo-Hee Lee, Sun Kyoung Song, Na-Young Son, Seung Hwa Kim, Ki Rim Chung, Won-Yoon Int J Mol Sci Article Chemerin is secreted as prochemerin from various cell types and then cleaved into the bioactive isoform by specific proteases. In various cancer types, chemerin exhibits pro- or antitumor effects. In the present study, chemerin treatment significantly inhibited the viability and invasion of breast cancer cells in the absence or presence of transforming growth factor (TGF)-β and insulin-like growth factor (IGF)-1. The expression levels of E-cadherin and vimentin were reduced in chemerin-treated breast cancer cells. However, chemerin treatment recovered the reduced E-cadherin expression level in breast cancer cells treated with TGF-β or IGF-1. Chemerin treatment inhibited nuclear β-catenin levels in breast cancer cells stimulated with or without TGF-β or IGF-1. In addition, chemerin treatment blocked the increase in the receptor activator of nuclear factor kappa-Β ligand (RANKL)/osteoprotegerin (OPG) ratio in osteoblastic cells exposed to metastatic breast cancer cell-derived conditioned medium. Chemerin treatment inhibited RANKL-induced osteoclast formation and bone resorption by reducing the secretion of matrix metalloproteinase (MMP)-2, MMP-9, and cathepsin K. Intraperitoneal administration of chemerin inhibited tumor growth in MCF-7 breast cancer cell-injected mice and reduced the development of osteolytic lesions resulting from intratibial inoculation of MDA-MB-231 cells. Taken together, chemerin inhibits the growth and invasion of breast cancer cells and prevents bone loss resulting from breast cancer cells by inhibiting finally osteoclast formation and activity. MDPI 2020-04-20 /pmc/articles/PMC7216174/ /pubmed/32325994 http://dx.doi.org/10.3390/ijms21082871 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Hyungkeun
Lee, Joo-Hee
Lee, Sun Kyoung
Song, Na-Young
Son, Seung Hwa
Kim, Ki Rim
Chung, Won-Yoon
Chemerin Treatment Inhibits the Growth and Bone Invasion of Breast Cancer Cells
title Chemerin Treatment Inhibits the Growth and Bone Invasion of Breast Cancer Cells
title_full Chemerin Treatment Inhibits the Growth and Bone Invasion of Breast Cancer Cells
title_fullStr Chemerin Treatment Inhibits the Growth and Bone Invasion of Breast Cancer Cells
title_full_unstemmed Chemerin Treatment Inhibits the Growth and Bone Invasion of Breast Cancer Cells
title_short Chemerin Treatment Inhibits the Growth and Bone Invasion of Breast Cancer Cells
title_sort chemerin treatment inhibits the growth and bone invasion of breast cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216174/
https://www.ncbi.nlm.nih.gov/pubmed/32325994
http://dx.doi.org/10.3390/ijms21082871
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