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Tree peony variegated flowers show a small insertion in the F3’H gene of the acyanic flower parts
BACKGROUND: The tree peony (Paeonia suffruticosa Andr.) cultivar ‘Er Qiao’ is appreciated for its unstable variegated flower coloration, with cyanic and acyanic flowers appearing on different branches of the same plant and occasionally in a single flower or petal. However, the variegation mechanism...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216414/ https://www.ncbi.nlm.nih.gov/pubmed/32398153 http://dx.doi.org/10.1186/s12870-020-02428-x |
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author | Zhang, Yanzhao Cheng, Yanwei Xu, Shuzhen Ma, Huiping Han, Jianming Zhang, Yan |
author_facet | Zhang, Yanzhao Cheng, Yanwei Xu, Shuzhen Ma, Huiping Han, Jianming Zhang, Yan |
author_sort | Zhang, Yanzhao |
collection | PubMed |
description | BACKGROUND: The tree peony (Paeonia suffruticosa Andr.) cultivar ‘Er Qiao’ is appreciated for its unstable variegated flower coloration, with cyanic and acyanic flowers appearing on different branches of the same plant and occasionally in a single flower or petal. However, the variegation mechanism is still unclear. RESULTS: In this study, we found significantly higher contents and more diverse sets of anthocyanins in the cyanic petals than in the acyanic petals. Comparative transcriptome analysis between the two flower types revealed 477 differentially expressed genes (DEGs). Quantitative real-time PCR results verified that the transcript levels of the flavonol synthase (FLS) gene were significantly increased in the acyanic petals. Furthermore, we found that a GCGGCG insertion at 246 bp in the flavonoid 3′-hydroxylase (F3’H) gene-coding region constitutes a duplication of the 241–245 bp section and was consistently found only in acyanic flowers. Sequence alignment of the F3’H gene from different plant species indicated that only the acyanic petals of ‘Er Qiao’ contained the GCGGCG insertion. The transformation of Arabidopsis tt7–1 lines demonstrated that the ectopic expression of F3’H-cyanic, but not F3’H-acyanic, could complement the colors in the hypocotyl and seed coat. CONCLUSION: In summary, we found that an indel in F3’H and the upregulation of FLS drastically reduced the anthocyanin content in acyanic petals. Our results provide molecular candidates for a better understanding of the variegation mechanisms in tree peony. |
format | Online Article Text |
id | pubmed-7216414 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-72164142020-05-18 Tree peony variegated flowers show a small insertion in the F3’H gene of the acyanic flower parts Zhang, Yanzhao Cheng, Yanwei Xu, Shuzhen Ma, Huiping Han, Jianming Zhang, Yan BMC Plant Biol Research Article BACKGROUND: The tree peony (Paeonia suffruticosa Andr.) cultivar ‘Er Qiao’ is appreciated for its unstable variegated flower coloration, with cyanic and acyanic flowers appearing on different branches of the same plant and occasionally in a single flower or petal. However, the variegation mechanism is still unclear. RESULTS: In this study, we found significantly higher contents and more diverse sets of anthocyanins in the cyanic petals than in the acyanic petals. Comparative transcriptome analysis between the two flower types revealed 477 differentially expressed genes (DEGs). Quantitative real-time PCR results verified that the transcript levels of the flavonol synthase (FLS) gene were significantly increased in the acyanic petals. Furthermore, we found that a GCGGCG insertion at 246 bp in the flavonoid 3′-hydroxylase (F3’H) gene-coding region constitutes a duplication of the 241–245 bp section and was consistently found only in acyanic flowers. Sequence alignment of the F3’H gene from different plant species indicated that only the acyanic petals of ‘Er Qiao’ contained the GCGGCG insertion. The transformation of Arabidopsis tt7–1 lines demonstrated that the ectopic expression of F3’H-cyanic, but not F3’H-acyanic, could complement the colors in the hypocotyl and seed coat. CONCLUSION: In summary, we found that an indel in F3’H and the upregulation of FLS drastically reduced the anthocyanin content in acyanic petals. Our results provide molecular candidates for a better understanding of the variegation mechanisms in tree peony. BioMed Central 2020-05-12 /pmc/articles/PMC7216414/ /pubmed/32398153 http://dx.doi.org/10.1186/s12870-020-02428-x Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Zhang, Yanzhao Cheng, Yanwei Xu, Shuzhen Ma, Huiping Han, Jianming Zhang, Yan Tree peony variegated flowers show a small insertion in the F3’H gene of the acyanic flower parts |
title | Tree peony variegated flowers show a small insertion in the F3’H gene of the acyanic flower parts |
title_full | Tree peony variegated flowers show a small insertion in the F3’H gene of the acyanic flower parts |
title_fullStr | Tree peony variegated flowers show a small insertion in the F3’H gene of the acyanic flower parts |
title_full_unstemmed | Tree peony variegated flowers show a small insertion in the F3’H gene of the acyanic flower parts |
title_short | Tree peony variegated flowers show a small insertion in the F3’H gene of the acyanic flower parts |
title_sort | tree peony variegated flowers show a small insertion in the f3’h gene of the acyanic flower parts |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216414/ https://www.ncbi.nlm.nih.gov/pubmed/32398153 http://dx.doi.org/10.1186/s12870-020-02428-x |
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