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Concentration patterns of antibacterial factors and immunoglobulin A antibody in foremilk fractions of healthy cows

Antibacterial factors act as innate immune components, which respond as soon as bacteria enter a living organism. To prevent and treat mastitis in cattle, understanding the concentrations of these substances inside the udder is important; however, they remain to be studied. In this investigation, th...

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Autores principales: Kitano, Nana, Isobe, Naoki, Noda, Jun, Takahashi, Toshihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216940/
https://www.ncbi.nlm.nih.gov/pubmed/32285528
http://dx.doi.org/10.1111/asj.13372
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author Kitano, Nana
Isobe, Naoki
Noda, Jun
Takahashi, Toshihiko
author_facet Kitano, Nana
Isobe, Naoki
Noda, Jun
Takahashi, Toshihiko
author_sort Kitano, Nana
collection PubMed
description Antibacterial factors act as innate immune components, which respond as soon as bacteria enter a living organism. To prevent and treat mastitis in cattle, understanding the concentrations of these substances inside the udder is important; however, they remain to be studied. In this investigation, the concentration of lingual antimicrobial peptide (LAP), S100 protein (S100A7), lactoferrin (LF), and immunoglobulin antibody were measured in the different fractions of foremilk. Lactating Holstein cows were examined, and 10 foremilk fractions were obtained from sequential samples up to 150 ml. The LAP concentrations in milk samples increased until 25 ml. The LF concentrations increased up to the 10 ml fraction, then stabilized at low level after the 50 ml fraction. For S100A7, some fractions had significantly higher (p < .05) concentrations than the 5 or 10 ml fractions. The IgA antibody concentration increased up to the 5 ml fraction, then after 50 ml fraction showed relatively low concentrations. This investigation determined the concentration patterns of LAP, LF, S100A7, and IgA antibody secreted in milk inside the udders of healthy lactating cows as baseline data. These distinct concentration patterns might indicate various protective responses.
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spelling pubmed-72169402020-05-13 Concentration patterns of antibacterial factors and immunoglobulin A antibody in foremilk fractions of healthy cows Kitano, Nana Isobe, Naoki Noda, Jun Takahashi, Toshihiko Anim Sci J Original Articles Antibacterial factors act as innate immune components, which respond as soon as bacteria enter a living organism. To prevent and treat mastitis in cattle, understanding the concentrations of these substances inside the udder is important; however, they remain to be studied. In this investigation, the concentration of lingual antimicrobial peptide (LAP), S100 protein (S100A7), lactoferrin (LF), and immunoglobulin antibody were measured in the different fractions of foremilk. Lactating Holstein cows were examined, and 10 foremilk fractions were obtained from sequential samples up to 150 ml. The LAP concentrations in milk samples increased until 25 ml. The LF concentrations increased up to the 10 ml fraction, then stabilized at low level after the 50 ml fraction. For S100A7, some fractions had significantly higher (p < .05) concentrations than the 5 or 10 ml fractions. The IgA antibody concentration increased up to the 5 ml fraction, then after 50 ml fraction showed relatively low concentrations. This investigation determined the concentration patterns of LAP, LF, S100A7, and IgA antibody secreted in milk inside the udders of healthy lactating cows as baseline data. These distinct concentration patterns might indicate various protective responses. John Wiley and Sons Inc. 2020-04-13 2020 /pmc/articles/PMC7216940/ /pubmed/32285528 http://dx.doi.org/10.1111/asj.13372 Text en © 2020 The Authors. Animal Science Journal published by John Wiley & Sons Australia, Ltd on behalf of Japanese Society of Animal Science. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Kitano, Nana
Isobe, Naoki
Noda, Jun
Takahashi, Toshihiko
Concentration patterns of antibacterial factors and immunoglobulin A antibody in foremilk fractions of healthy cows
title Concentration patterns of antibacterial factors and immunoglobulin A antibody in foremilk fractions of healthy cows
title_full Concentration patterns of antibacterial factors and immunoglobulin A antibody in foremilk fractions of healthy cows
title_fullStr Concentration patterns of antibacterial factors and immunoglobulin A antibody in foremilk fractions of healthy cows
title_full_unstemmed Concentration patterns of antibacterial factors and immunoglobulin A antibody in foremilk fractions of healthy cows
title_short Concentration patterns of antibacterial factors and immunoglobulin A antibody in foremilk fractions of healthy cows
title_sort concentration patterns of antibacterial factors and immunoglobulin a antibody in foremilk fractions of healthy cows
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216940/
https://www.ncbi.nlm.nih.gov/pubmed/32285528
http://dx.doi.org/10.1111/asj.13372
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