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Host interactors of effector proteins of the lettuce downy mildew Bremia lactucae obtained by yeast two-hybrid screening
Plant pathogenic bacteria, fungi and oomycetes secrete effector proteins to manipulate host cell processes to establish a successful infection. Over the last decade the genomes and transcriptomes of many agriculturally important plant pathogens have been sequenced and vast candidate effector reperto...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7217486/ https://www.ncbi.nlm.nih.gov/pubmed/32396563 http://dx.doi.org/10.1371/journal.pone.0226540 |
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author | Pelgrom, Alexandra J. E. Meisrimler, Claudia-Nicole Elberse, Joyce Koorman, Thijs Boxem, Mike Van den Ackerveken, Guido |
author_facet | Pelgrom, Alexandra J. E. Meisrimler, Claudia-Nicole Elberse, Joyce Koorman, Thijs Boxem, Mike Van den Ackerveken, Guido |
author_sort | Pelgrom, Alexandra J. E. |
collection | PubMed |
description | Plant pathogenic bacteria, fungi and oomycetes secrete effector proteins to manipulate host cell processes to establish a successful infection. Over the last decade the genomes and transcriptomes of many agriculturally important plant pathogens have been sequenced and vast candidate effector repertoires were identified using bioinformatic analyses. Elucidating the contribution of individual effectors to pathogenicity is the next major hurdle. To advance our understanding of the molecular mechanisms underlying lettuce susceptibility to the downy mildew Bremia lactucae, we mapped physical interactions between B. lactucae effectors and lettuce candidate target proteins. Using a lettuce cDNA library-based yeast-two-hybrid system, 61 protein-protein interactions were identified, involving 21 B. lactucae effectors and 46 unique lettuce proteins. The top ten interactors based on the number of independent colonies identified in the Y2H and two interactors that belong to gene families involved in plant immunity, were further characterized. We determined the subcellular localization of the fluorescently tagged lettuce proteins and their interacting effectors. Importantly, relocalization of effectors or their interactors to the nucleus was observed for four protein-pairs upon their co-expression, supporting their interaction in planta. |
format | Online Article Text |
id | pubmed-7217486 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-72174862020-05-29 Host interactors of effector proteins of the lettuce downy mildew Bremia lactucae obtained by yeast two-hybrid screening Pelgrom, Alexandra J. E. Meisrimler, Claudia-Nicole Elberse, Joyce Koorman, Thijs Boxem, Mike Van den Ackerveken, Guido PLoS One Research Article Plant pathogenic bacteria, fungi and oomycetes secrete effector proteins to manipulate host cell processes to establish a successful infection. Over the last decade the genomes and transcriptomes of many agriculturally important plant pathogens have been sequenced and vast candidate effector repertoires were identified using bioinformatic analyses. Elucidating the contribution of individual effectors to pathogenicity is the next major hurdle. To advance our understanding of the molecular mechanisms underlying lettuce susceptibility to the downy mildew Bremia lactucae, we mapped physical interactions between B. lactucae effectors and lettuce candidate target proteins. Using a lettuce cDNA library-based yeast-two-hybrid system, 61 protein-protein interactions were identified, involving 21 B. lactucae effectors and 46 unique lettuce proteins. The top ten interactors based on the number of independent colonies identified in the Y2H and two interactors that belong to gene families involved in plant immunity, were further characterized. We determined the subcellular localization of the fluorescently tagged lettuce proteins and their interacting effectors. Importantly, relocalization of effectors or their interactors to the nucleus was observed for four protein-pairs upon their co-expression, supporting their interaction in planta. Public Library of Science 2020-05-12 /pmc/articles/PMC7217486/ /pubmed/32396563 http://dx.doi.org/10.1371/journal.pone.0226540 Text en © 2020 Pelgrom et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Pelgrom, Alexandra J. E. Meisrimler, Claudia-Nicole Elberse, Joyce Koorman, Thijs Boxem, Mike Van den Ackerveken, Guido Host interactors of effector proteins of the lettuce downy mildew Bremia lactucae obtained by yeast two-hybrid screening |
title | Host interactors of effector proteins of the lettuce downy mildew Bremia lactucae obtained by yeast two-hybrid screening |
title_full | Host interactors of effector proteins of the lettuce downy mildew Bremia lactucae obtained by yeast two-hybrid screening |
title_fullStr | Host interactors of effector proteins of the lettuce downy mildew Bremia lactucae obtained by yeast two-hybrid screening |
title_full_unstemmed | Host interactors of effector proteins of the lettuce downy mildew Bremia lactucae obtained by yeast two-hybrid screening |
title_short | Host interactors of effector proteins of the lettuce downy mildew Bremia lactucae obtained by yeast two-hybrid screening |
title_sort | host interactors of effector proteins of the lettuce downy mildew bremia lactucae obtained by yeast two-hybrid screening |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7217486/ https://www.ncbi.nlm.nih.gov/pubmed/32396563 http://dx.doi.org/10.1371/journal.pone.0226540 |
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