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Housefly (Musca domestica L.) associated microbiota across different life stages
The housefly (Musca domestica L.) lives in close association with its microbiota and its symbionts are suggested to have pivotal roles in processes such as metabolism and immune response, but it is unclear how the profound physiological changes during ontogeny affect the housefly’s associated microb...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7217826/ https://www.ncbi.nlm.nih.gov/pubmed/32398740 http://dx.doi.org/10.1038/s41598-020-64704-y |
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author | de Jonge, Nadieh Michaelsen, Thomas Yssing Ejbye-Ernst, Rasmus Jensen, Anne Nielsen, Majken Elley Bahrndorff, Simon Nielsen, Jeppe Lund |
author_facet | de Jonge, Nadieh Michaelsen, Thomas Yssing Ejbye-Ernst, Rasmus Jensen, Anne Nielsen, Majken Elley Bahrndorff, Simon Nielsen, Jeppe Lund |
author_sort | de Jonge, Nadieh |
collection | PubMed |
description | The housefly (Musca domestica L.) lives in close association with its microbiota and its symbionts are suggested to have pivotal roles in processes such as metabolism and immune response, but it is unclear how the profound physiological changes during ontogeny affect the housefly’s associated microbiota and their metabolic capabilities. The present study applies 16S rRNA gene amplicon sequencing to investigate the development of the host-associated microbiota during ontogeny. The potential for microbiota transfer between developmental stages, and the metabolic potential of these microbiota were evaluated. Representatives of Firmicutes were observed as early colonisers during the larval stages, followed by colonisation by organisms affiliating with Proteobacteria and Bacteroidetes as the flies matured into adults. Microbiota observed across all the developmental stages included Lactococcus, Lactobacillus and Enterococcus, while Weissella and Chishuiella were associated with newly hatched larvae and adults, respectively. Predictive metabolic profiling of the identified microorganisms further suggested that the microbiota and their functional profile mature alongside their host and putative host-microbe relationships are established at different stages of development. The predicted metabolic capability of the microbiota developed from primarily simple processes including carbohydrate and nucleotide metabolisms, to more complex metabolic pathways including amino acid metabolisms and processes related to signal transduction. |
format | Online Article Text |
id | pubmed-7217826 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-72178262020-05-19 Housefly (Musca domestica L.) associated microbiota across different life stages de Jonge, Nadieh Michaelsen, Thomas Yssing Ejbye-Ernst, Rasmus Jensen, Anne Nielsen, Majken Elley Bahrndorff, Simon Nielsen, Jeppe Lund Sci Rep Article The housefly (Musca domestica L.) lives in close association with its microbiota and its symbionts are suggested to have pivotal roles in processes such as metabolism and immune response, but it is unclear how the profound physiological changes during ontogeny affect the housefly’s associated microbiota and their metabolic capabilities. The present study applies 16S rRNA gene amplicon sequencing to investigate the development of the host-associated microbiota during ontogeny. The potential for microbiota transfer between developmental stages, and the metabolic potential of these microbiota were evaluated. Representatives of Firmicutes were observed as early colonisers during the larval stages, followed by colonisation by organisms affiliating with Proteobacteria and Bacteroidetes as the flies matured into adults. Microbiota observed across all the developmental stages included Lactococcus, Lactobacillus and Enterococcus, while Weissella and Chishuiella were associated with newly hatched larvae and adults, respectively. Predictive metabolic profiling of the identified microorganisms further suggested that the microbiota and their functional profile mature alongside their host and putative host-microbe relationships are established at different stages of development. The predicted metabolic capability of the microbiota developed from primarily simple processes including carbohydrate and nucleotide metabolisms, to more complex metabolic pathways including amino acid metabolisms and processes related to signal transduction. Nature Publishing Group UK 2020-05-12 /pmc/articles/PMC7217826/ /pubmed/32398740 http://dx.doi.org/10.1038/s41598-020-64704-y Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article de Jonge, Nadieh Michaelsen, Thomas Yssing Ejbye-Ernst, Rasmus Jensen, Anne Nielsen, Majken Elley Bahrndorff, Simon Nielsen, Jeppe Lund Housefly (Musca domestica L.) associated microbiota across different life stages |
title | Housefly (Musca domestica L.) associated microbiota across different life stages |
title_full | Housefly (Musca domestica L.) associated microbiota across different life stages |
title_fullStr | Housefly (Musca domestica L.) associated microbiota across different life stages |
title_full_unstemmed | Housefly (Musca domestica L.) associated microbiota across different life stages |
title_short | Housefly (Musca domestica L.) associated microbiota across different life stages |
title_sort | housefly (musca domestica l.) associated microbiota across different life stages |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7217826/ https://www.ncbi.nlm.nih.gov/pubmed/32398740 http://dx.doi.org/10.1038/s41598-020-64704-y |
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