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ARC(AgRP/NPY) Neuron Activity Is Required for Acute Exercise-Induced Food Intake in Un-Trained Mice

While much is known about the role of agouti-regulated peptide/neuropeptide Y (AgRP/NPY) and pro-opiomelanocortin (POMC) neurons to regulate energy homeostasis, little is known about how forced energy expenditure, such as exercise, modulates these neurons and if these neurons are involved in post-ex...

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Autores principales: Bunner, Wyatt, Landry, Taylor, Laing, Brenton Thomas, Li, Peixin, Rao, Zhijian, Yuan, Yuan, Huang, Hu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218131/
https://www.ncbi.nlm.nih.gov/pubmed/32435204
http://dx.doi.org/10.3389/fphys.2020.00411
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author Bunner, Wyatt
Landry, Taylor
Laing, Brenton Thomas
Li, Peixin
Rao, Zhijian
Yuan, Yuan
Huang, Hu
author_facet Bunner, Wyatt
Landry, Taylor
Laing, Brenton Thomas
Li, Peixin
Rao, Zhijian
Yuan, Yuan
Huang, Hu
author_sort Bunner, Wyatt
collection PubMed
description While much is known about the role of agouti-regulated peptide/neuropeptide Y (AgRP/NPY) and pro-opiomelanocortin (POMC) neurons to regulate energy homeostasis, little is known about how forced energy expenditure, such as exercise, modulates these neurons and if these neurons are involved in post-exercise feeding behaviors. We utilized multiple mouse models to investigate the effects of acute, moderate-intensity exercise on food intake and neuronal activity in the arcuate nucleus (ARC) of the hypothalamus. NPY-GFP reporter mice were utilized for immunohistochemistry and patch-clamp electrophysiology experiments investigating neuronal activation immediately after acute treadmill exercise. Additionally, ARC(AgRP/NPY) neuron inhibition was performed using the Designer Receptors Exclusively Activated by Designer Drugs (DREADD) system in AgRP-Cre transgenic mice to investigate the importance of AgRP/NPY neurons in post-exercise feeding behaviors. Our experiments revealed that acute moderate-intensity exercise significantly increased food intake, ARC(AgRP/NPY) neuron activation, and PVN(Sim1) neuron activation, while having no effect on ARC(POMC) neurons. Strikingly, this exercise-induced refeeding was completely abolished when ARC(AgRP/NPY) neuron activity was inhibited. While acute exercise also increased PVN(Sim1) neuron activity, inhibition of ARC(AgRP/NPY) neurons had no effect on PVN(Sim1) neuronal activation. Overall, our results reveal that ARC(AgRP/NPY) activation is required for acute exercise induced food intake in mice, thus providing insight into the critical role of ARC(AgRP/NPY) neurons in maintaining energy homeostasis in cases of exercise-mediated energy deficit.
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spelling pubmed-72181312020-05-20 ARC(AgRP/NPY) Neuron Activity Is Required for Acute Exercise-Induced Food Intake in Un-Trained Mice Bunner, Wyatt Landry, Taylor Laing, Brenton Thomas Li, Peixin Rao, Zhijian Yuan, Yuan Huang, Hu Front Physiol Physiology While much is known about the role of agouti-regulated peptide/neuropeptide Y (AgRP/NPY) and pro-opiomelanocortin (POMC) neurons to regulate energy homeostasis, little is known about how forced energy expenditure, such as exercise, modulates these neurons and if these neurons are involved in post-exercise feeding behaviors. We utilized multiple mouse models to investigate the effects of acute, moderate-intensity exercise on food intake and neuronal activity in the arcuate nucleus (ARC) of the hypothalamus. NPY-GFP reporter mice were utilized for immunohistochemistry and patch-clamp electrophysiology experiments investigating neuronal activation immediately after acute treadmill exercise. Additionally, ARC(AgRP/NPY) neuron inhibition was performed using the Designer Receptors Exclusively Activated by Designer Drugs (DREADD) system in AgRP-Cre transgenic mice to investigate the importance of AgRP/NPY neurons in post-exercise feeding behaviors. Our experiments revealed that acute moderate-intensity exercise significantly increased food intake, ARC(AgRP/NPY) neuron activation, and PVN(Sim1) neuron activation, while having no effect on ARC(POMC) neurons. Strikingly, this exercise-induced refeeding was completely abolished when ARC(AgRP/NPY) neuron activity was inhibited. While acute exercise also increased PVN(Sim1) neuron activity, inhibition of ARC(AgRP/NPY) neurons had no effect on PVN(Sim1) neuronal activation. Overall, our results reveal that ARC(AgRP/NPY) activation is required for acute exercise induced food intake in mice, thus providing insight into the critical role of ARC(AgRP/NPY) neurons in maintaining energy homeostasis in cases of exercise-mediated energy deficit. Frontiers Media S.A. 2020-05-06 /pmc/articles/PMC7218131/ /pubmed/32435204 http://dx.doi.org/10.3389/fphys.2020.00411 Text en Copyright © 2020 Bunner, Landry, Laing, Li, Rao, Yuan and Huang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Bunner, Wyatt
Landry, Taylor
Laing, Brenton Thomas
Li, Peixin
Rao, Zhijian
Yuan, Yuan
Huang, Hu
ARC(AgRP/NPY) Neuron Activity Is Required for Acute Exercise-Induced Food Intake in Un-Trained Mice
title ARC(AgRP/NPY) Neuron Activity Is Required for Acute Exercise-Induced Food Intake in Un-Trained Mice
title_full ARC(AgRP/NPY) Neuron Activity Is Required for Acute Exercise-Induced Food Intake in Un-Trained Mice
title_fullStr ARC(AgRP/NPY) Neuron Activity Is Required for Acute Exercise-Induced Food Intake in Un-Trained Mice
title_full_unstemmed ARC(AgRP/NPY) Neuron Activity Is Required for Acute Exercise-Induced Food Intake in Un-Trained Mice
title_short ARC(AgRP/NPY) Neuron Activity Is Required for Acute Exercise-Induced Food Intake in Un-Trained Mice
title_sort arc(agrp/npy) neuron activity is required for acute exercise-induced food intake in un-trained mice
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218131/
https://www.ncbi.nlm.nih.gov/pubmed/32435204
http://dx.doi.org/10.3389/fphys.2020.00411
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