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Visual and simple determination of glucose-induced acidification by yeast cells: application to rapid cytotoxicity test
This study demonstrated that glucose-induced proton release from yeast cells was more sensitive to various inhibitors than cell proliferation. In this study the inhibition of glucose-induced proton release was determined on the basis of color change of pH indicator, methyl red, from pH 5 to pH6 at c...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218269/ https://www.ncbi.nlm.nih.gov/pubmed/32420490 http://dx.doi.org/10.1016/j.heliyon.2020.e03924 |
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author | Yamashoji, Shiro Al Mamun, Arafat Bari, Latiful |
author_facet | Yamashoji, Shiro Al Mamun, Arafat Bari, Latiful |
author_sort | Yamashoji, Shiro |
collection | PubMed |
description | This study demonstrated that glucose-induced proton release from yeast cells was more sensitive to various inhibitors than cell proliferation. In this study the inhibition of glucose-induced proton release was determined on the basis of color change of pH indicator, methyl red, from pH 5 to pH6 at cell density of 2.5 × 10(7) cells/ml. When yeast cells were incubated with the inhibitors of glucose intake, glycolysis, and plasma membrane H (+) -ATPase for 1 h, these cytotoxic effects were observed by following the change in absorbance at 527 nm due to methyl red for 5 min. The cytotoxic effects of heavy metal ions, detergents and quinones were observed in the same manner. The above method was superior in sensitivity and measurement time to cell proliferation measurement that required 9 h. This visual cytotoxicity test (methyl red test) is expected to be useful as simple and rapid cytotoxicity test with yeast cells. |
format | Online Article Text |
id | pubmed-7218269 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-72182692020-05-15 Visual and simple determination of glucose-induced acidification by yeast cells: application to rapid cytotoxicity test Yamashoji, Shiro Al Mamun, Arafat Bari, Latiful Heliyon Article This study demonstrated that glucose-induced proton release from yeast cells was more sensitive to various inhibitors than cell proliferation. In this study the inhibition of glucose-induced proton release was determined on the basis of color change of pH indicator, methyl red, from pH 5 to pH6 at cell density of 2.5 × 10(7) cells/ml. When yeast cells were incubated with the inhibitors of glucose intake, glycolysis, and plasma membrane H (+) -ATPase for 1 h, these cytotoxic effects were observed by following the change in absorbance at 527 nm due to methyl red for 5 min. The cytotoxic effects of heavy metal ions, detergents and quinones were observed in the same manner. The above method was superior in sensitivity and measurement time to cell proliferation measurement that required 9 h. This visual cytotoxicity test (methyl red test) is expected to be useful as simple and rapid cytotoxicity test with yeast cells. Elsevier 2020-05-11 /pmc/articles/PMC7218269/ /pubmed/32420490 http://dx.doi.org/10.1016/j.heliyon.2020.e03924 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Yamashoji, Shiro Al Mamun, Arafat Bari, Latiful Visual and simple determination of glucose-induced acidification by yeast cells: application to rapid cytotoxicity test |
title | Visual and simple determination of glucose-induced acidification by yeast cells: application to rapid cytotoxicity test |
title_full | Visual and simple determination of glucose-induced acidification by yeast cells: application to rapid cytotoxicity test |
title_fullStr | Visual and simple determination of glucose-induced acidification by yeast cells: application to rapid cytotoxicity test |
title_full_unstemmed | Visual and simple determination of glucose-induced acidification by yeast cells: application to rapid cytotoxicity test |
title_short | Visual and simple determination of glucose-induced acidification by yeast cells: application to rapid cytotoxicity test |
title_sort | visual and simple determination of glucose-induced acidification by yeast cells: application to rapid cytotoxicity test |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218269/ https://www.ncbi.nlm.nih.gov/pubmed/32420490 http://dx.doi.org/10.1016/j.heliyon.2020.e03924 |
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