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Neuroprotective Effect of Activated Protein C on Blood–Brain Barrier Injury During Focal Cerebral Ischemia/Reperfusion
Although the effect of activated protein C (APC) on neuronal injury and neuroinflammatory responses has been extensively studied, the detailed mechanism underlying APC-protective effect in the blood–brain barrier (BBB) injury during ischemia is still not clear. In this study, the APC effect against...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218308/ https://www.ncbi.nlm.nih.gov/pubmed/32425720 http://dx.doi.org/10.1177/1559325820917288 |
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author | Wang, Jinqiao Rao, Gaofeng Ma, Yifan Zhang, Jingjing Shen, Jingjing Shi, Chaohong |
author_facet | Wang, Jinqiao Rao, Gaofeng Ma, Yifan Zhang, Jingjing Shen, Jingjing Shi, Chaohong |
author_sort | Wang, Jinqiao |
collection | PubMed |
description | Although the effect of activated protein C (APC) on neuronal injury and neuroinflammatory responses has been extensively studied, the detailed mechanism underlying APC-protective effect in the blood–brain barrier (BBB) injury during ischemia is still not clear. In this study, the APC effect against neuroinflammatory responses was evaluated in the model of right middle cerebral artery occlusion in male Sprague-Dawley rats with 2 hours of ischemia and 22 hours of reperfusion. The results showed that APC can significantly improve the neurological function scoring and reduce the infarct volume and BBB permeability. Moreover, the expression of protein nuclear factor-kappa B (NF-κB), both in cytoplasm and nuclei, was reduced. The downstream of NF-κB activation, including tumor necrosis factor-α and interleukin-1β secretion, was inhibited. In all, APC exerts a neuroprotective effect in focal cerebral ischemia–reperfusion in rats by inhibiting the activation and nuclear translocation of NF-κB. It may indicate a therapeutic approach for ischemic brain injury. |
format | Online Article Text |
id | pubmed-7218308 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-72183082020-05-18 Neuroprotective Effect of Activated Protein C on Blood–Brain Barrier Injury During Focal Cerebral Ischemia/Reperfusion Wang, Jinqiao Rao, Gaofeng Ma, Yifan Zhang, Jingjing Shen, Jingjing Shi, Chaohong Dose Response Nanotechnology and Microtechnology in Drug Delivery Systems Although the effect of activated protein C (APC) on neuronal injury and neuroinflammatory responses has been extensively studied, the detailed mechanism underlying APC-protective effect in the blood–brain barrier (BBB) injury during ischemia is still not clear. In this study, the APC effect against neuroinflammatory responses was evaluated in the model of right middle cerebral artery occlusion in male Sprague-Dawley rats with 2 hours of ischemia and 22 hours of reperfusion. The results showed that APC can significantly improve the neurological function scoring and reduce the infarct volume and BBB permeability. Moreover, the expression of protein nuclear factor-kappa B (NF-κB), both in cytoplasm and nuclei, was reduced. The downstream of NF-κB activation, including tumor necrosis factor-α and interleukin-1β secretion, was inhibited. In all, APC exerts a neuroprotective effect in focal cerebral ischemia–reperfusion in rats by inhibiting the activation and nuclear translocation of NF-κB. It may indicate a therapeutic approach for ischemic brain injury. SAGE Publications 2020-05-04 /pmc/articles/PMC7218308/ /pubmed/32425720 http://dx.doi.org/10.1177/1559325820917288 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Nanotechnology and Microtechnology in Drug Delivery Systems Wang, Jinqiao Rao, Gaofeng Ma, Yifan Zhang, Jingjing Shen, Jingjing Shi, Chaohong Neuroprotective Effect of Activated Protein C on Blood–Brain Barrier Injury During Focal Cerebral Ischemia/Reperfusion |
title | Neuroprotective Effect of Activated Protein C on Blood–Brain Barrier Injury During Focal Cerebral Ischemia/Reperfusion |
title_full | Neuroprotective Effect of Activated Protein C on Blood–Brain Barrier Injury During Focal Cerebral Ischemia/Reperfusion |
title_fullStr | Neuroprotective Effect of Activated Protein C on Blood–Brain Barrier Injury During Focal Cerebral Ischemia/Reperfusion |
title_full_unstemmed | Neuroprotective Effect of Activated Protein C on Blood–Brain Barrier Injury During Focal Cerebral Ischemia/Reperfusion |
title_short | Neuroprotective Effect of Activated Protein C on Blood–Brain Barrier Injury During Focal Cerebral Ischemia/Reperfusion |
title_sort | neuroprotective effect of activated protein c on blood–brain barrier injury during focal cerebral ischemia/reperfusion |
topic | Nanotechnology and Microtechnology in Drug Delivery Systems |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218308/ https://www.ncbi.nlm.nih.gov/pubmed/32425720 http://dx.doi.org/10.1177/1559325820917288 |
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