Network Analysis of Murine Cortical Dynamics Implicates Untuned Neurons in Visual Stimulus Coding

Unbiased and dense sampling of large populations of layer 2/3 pyramidal neurons in mouse primary visual cortex (V1) reveals two functional sub-populations: neurons tuned and untuned to drifting gratings. Whether functional interactions between these two groups contribute to the representation of visual stimuli is unclear. To examine these interactions, we summarize the population partial pairwise correlation structure as a directed and weighted graph. We find that tuned and untuned neurons have distinct topological properties, with untuned neurons occupying central positions in functional networks (FNs). Implementation of a decoder that utilizes the topology of these FNs yields accurate decoding of visual stimuli. We further show that decoding performance degrades comparably following manipulations of either tuned or untuned neurons. Our results demonstrate that untuned neurons are an integral component of V1 FNs and suggest that network interactions contain information about the stimulus that is accessible to downstream elements.