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Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens

BACKGROUND: Careful consideration of experimental artefacts is required in order to successfully apply high-throughput 16S ribosomal ribonucleic acid (rRNA) gene sequencing technology. Here we introduce experimental design, quality control and “denoising” approaches for sequencing low biomass specim...

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Detalles Bibliográficos
Autores principales:	Claassen-Weitz, Shantelle, Gardner-Lubbe, Sugnet, Mwaikono, Kilaza S., du Toit, Elloise, Zar, Heather J., Nicol, Mark P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2020
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218582/ https://www.ncbi.nlm.nih.gov/pubmed/32397992 http://dx.doi.org/10.1186/s12866-020-01795-7

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