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Coumarin as a structural component of substrates and probes for serine and cysteine proteases
Coumarins represent well-established structures to introduce fluorescence into tool compounds for biochemical investigations. They are valued for their small size, chemical stability and accessibility as well as their tunable photochemical properties. As components of fluorophore/quencher pairs or F...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7219385/ https://www.ncbi.nlm.nih.gov/pubmed/32405284 http://dx.doi.org/10.1016/j.bbapap.2020.140445 |
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author | Breidenbach, Julian Bartz, Ulrike Gütschow, Michael |
author_facet | Breidenbach, Julian Bartz, Ulrike Gütschow, Michael |
author_sort | Breidenbach, Julian |
collection | PubMed |
description | Coumarins represent well-established structures to introduce fluorescence into tool compounds for biochemical investigations. They are valued for their small size, chemical stability and accessibility as well as their tunable photochemical properties. As components of fluorophore/quencher pairs or FRET donor/acceptor pairs, coumarins have frequently been applied in substrate mapping approaches for serine and cysteine proteases. This review also focuses on the incorporation of coumarins into the side chain of amino acids and the exploitation of the resulting fluorescent amino acids for the positional profiling of protease substrates. The protease-inhibiting properties of certain coumarin derivatives and the utilization of coumarin moieties to assemble activity-based probes for serine and cysteine proteases are discussed as well. |
format | Online Article Text |
id | pubmed-7219385 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72193852020-05-13 Coumarin as a structural component of substrates and probes for serine and cysteine proteases Breidenbach, Julian Bartz, Ulrike Gütschow, Michael Biochim Biophys Acta Proteins Proteom Article Coumarins represent well-established structures to introduce fluorescence into tool compounds for biochemical investigations. They are valued for their small size, chemical stability and accessibility as well as their tunable photochemical properties. As components of fluorophore/quencher pairs or FRET donor/acceptor pairs, coumarins have frequently been applied in substrate mapping approaches for serine and cysteine proteases. This review also focuses on the incorporation of coumarins into the side chain of amino acids and the exploitation of the resulting fluorescent amino acids for the positional profiling of protease substrates. The protease-inhibiting properties of certain coumarin derivatives and the utilization of coumarin moieties to assemble activity-based probes for serine and cysteine proteases are discussed as well. Elsevier B.V. 2020-09 2020-05-13 /pmc/articles/PMC7219385/ /pubmed/32405284 http://dx.doi.org/10.1016/j.bbapap.2020.140445 Text en © 2020 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Breidenbach, Julian Bartz, Ulrike Gütschow, Michael Coumarin as a structural component of substrates and probes for serine and cysteine proteases |
title | Coumarin as a structural component of substrates and probes for serine and cysteine proteases |
title_full | Coumarin as a structural component of substrates and probes for serine and cysteine proteases |
title_fullStr | Coumarin as a structural component of substrates and probes for serine and cysteine proteases |
title_full_unstemmed | Coumarin as a structural component of substrates and probes for serine and cysteine proteases |
title_short | Coumarin as a structural component of substrates and probes for serine and cysteine proteases |
title_sort | coumarin as a structural component of substrates and probes for serine and cysteine proteases |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7219385/ https://www.ncbi.nlm.nih.gov/pubmed/32405284 http://dx.doi.org/10.1016/j.bbapap.2020.140445 |
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