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Aging-associated sinus arrest and sick sinus syndrome in adult zebrafish
Because of its powerful genetics, the adult zebrafish has been increasingly used for studying cardiovascular diseases. Considering its heart rate of ~100 beats per minute at ambient temperature, which is very close to human, we assessed the use of this vertebrate animal for modeling heart rhythm dis...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7219707/ https://www.ncbi.nlm.nih.gov/pubmed/32401822 http://dx.doi.org/10.1371/journal.pone.0232457 |
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author | Yan, Jianhua Li, Hongsong Bu, Haisong Jiao, Kunli Zhang, Alex X. Le, Tai Cao, Hung Li, Yigang Ding, Yonghe Xu, Xiaolei |
author_facet | Yan, Jianhua Li, Hongsong Bu, Haisong Jiao, Kunli Zhang, Alex X. Le, Tai Cao, Hung Li, Yigang Ding, Yonghe Xu, Xiaolei |
author_sort | Yan, Jianhua |
collection | PubMed |
description | Because of its powerful genetics, the adult zebrafish has been increasingly used for studying cardiovascular diseases. Considering its heart rate of ~100 beats per minute at ambient temperature, which is very close to human, we assessed the use of this vertebrate animal for modeling heart rhythm disorders such as sinus arrest (SA) and sick sinus syndrome (SSS). We firstly optimized a protocol to measure electrocardiogram in adult zebrafish. We determined the location of the probes, implemented an open-chest microsurgery procedure, measured the effects of temperature, and determined appropriate anesthesia dose and time. We then proposed an PP interval of more than 1.5 seconds as an arbitrary criterion to define an SA episode in an adult fish at ambient temperature, based on comparison between the current definition of an SA episode in humans and our studies of candidate SA episodes in aged wild-type fish and Tg(SCN5A-D1275N) fish (a fish model for inherited SSS). With this criterion, a subpopulation of about 5% wild-type fish can be considered to have SA episodes, and this percentage significantly increases to about 25% in 3-year-old fish. In response to atropine, this subpopulation has both common SSS phenotypic traits that are shared with the Tg(SCN5A-D1275N) model, such as bradycardia; and unique SSS phenotypic traits, such as increased QRS/P ratio and chronotropic incompetence. In summary, this study defined baseline SA and SSS in adult zebrafish and underscored use of the zebrafish as an alternative model to study aging-associated SSS. |
format | Online Article Text |
id | pubmed-7219707 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-72197072020-05-29 Aging-associated sinus arrest and sick sinus syndrome in adult zebrafish Yan, Jianhua Li, Hongsong Bu, Haisong Jiao, Kunli Zhang, Alex X. Le, Tai Cao, Hung Li, Yigang Ding, Yonghe Xu, Xiaolei PLoS One Research Article Because of its powerful genetics, the adult zebrafish has been increasingly used for studying cardiovascular diseases. Considering its heart rate of ~100 beats per minute at ambient temperature, which is very close to human, we assessed the use of this vertebrate animal for modeling heart rhythm disorders such as sinus arrest (SA) and sick sinus syndrome (SSS). We firstly optimized a protocol to measure electrocardiogram in adult zebrafish. We determined the location of the probes, implemented an open-chest microsurgery procedure, measured the effects of temperature, and determined appropriate anesthesia dose and time. We then proposed an PP interval of more than 1.5 seconds as an arbitrary criterion to define an SA episode in an adult fish at ambient temperature, based on comparison between the current definition of an SA episode in humans and our studies of candidate SA episodes in aged wild-type fish and Tg(SCN5A-D1275N) fish (a fish model for inherited SSS). With this criterion, a subpopulation of about 5% wild-type fish can be considered to have SA episodes, and this percentage significantly increases to about 25% in 3-year-old fish. In response to atropine, this subpopulation has both common SSS phenotypic traits that are shared with the Tg(SCN5A-D1275N) model, such as bradycardia; and unique SSS phenotypic traits, such as increased QRS/P ratio and chronotropic incompetence. In summary, this study defined baseline SA and SSS in adult zebrafish and underscored use of the zebrafish as an alternative model to study aging-associated SSS. Public Library of Science 2020-05-13 /pmc/articles/PMC7219707/ /pubmed/32401822 http://dx.doi.org/10.1371/journal.pone.0232457 Text en © 2020 Yan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Yan, Jianhua Li, Hongsong Bu, Haisong Jiao, Kunli Zhang, Alex X. Le, Tai Cao, Hung Li, Yigang Ding, Yonghe Xu, Xiaolei Aging-associated sinus arrest and sick sinus syndrome in adult zebrafish |
title | Aging-associated sinus arrest and sick sinus syndrome in adult zebrafish |
title_full | Aging-associated sinus arrest and sick sinus syndrome in adult zebrafish |
title_fullStr | Aging-associated sinus arrest and sick sinus syndrome in adult zebrafish |
title_full_unstemmed | Aging-associated sinus arrest and sick sinus syndrome in adult zebrafish |
title_short | Aging-associated sinus arrest and sick sinus syndrome in adult zebrafish |
title_sort | aging-associated sinus arrest and sick sinus syndrome in adult zebrafish |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7219707/ https://www.ncbi.nlm.nih.gov/pubmed/32401822 http://dx.doi.org/10.1371/journal.pone.0232457 |
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