Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales)

The purpose of this study was to identify a reliable DNA extraction protocol to use on 25-year-old powdery mildew specimens from the reference collection VPRI in order to produce high quality sequences suitable to address taxonomic phylogenetic questions. We tested 13 extraction protocols and two li...

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Detalles Bibliográficos
Autores principales: Smith, Reannon L., Sawbridge, Tim, Mann, Ross, Kaur, Jatinder, May, Tom W., Edwards, Jacqueline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7219758/
https://www.ncbi.nlm.nih.gov/pubmed/32401807
http://dx.doi.org/10.1371/journal.pone.0232535
Descripción
Sumario:The purpose of this study was to identify a reliable DNA extraction protocol to use on 25-year-old powdery mildew specimens from the reference collection VPRI in order to produce high quality sequences suitable to address taxonomic phylogenetic questions. We tested 13 extraction protocols and two library preparation kits and found the combination of the E.Z.N.A.(®) Forensic DNA kit for DNA extraction and the NuGen Ovation(®) Ultralow System library preparation kit was the most suitable for this purpose.

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