Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales)

The purpose of this study was to identify a reliable DNA extraction protocol to use on 25-year-old powdery mildew specimens from the reference collection VPRI in order to produce high quality sequences suitable to address taxonomic phylogenetic questions. We tested 13 extraction protocols and two li...

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Detalles Bibliográficos
Autores principales: Smith, Reannon L., Sawbridge, Tim, Mann, Ross, Kaur, Jatinder, May, Tom W., Edwards, Jacqueline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7219758/
https://www.ncbi.nlm.nih.gov/pubmed/32401807
http://dx.doi.org/10.1371/journal.pone.0232535
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author Smith, Reannon L.
Sawbridge, Tim
Mann, Ross
Kaur, Jatinder
May, Tom W.
Edwards, Jacqueline
author_facet Smith, Reannon L.
Sawbridge, Tim
Mann, Ross
Kaur, Jatinder
May, Tom W.
Edwards, Jacqueline
author_sort Smith, Reannon L.
collection PubMed
description The purpose of this study was to identify a reliable DNA extraction protocol to use on 25-year-old powdery mildew specimens from the reference collection VPRI in order to produce high quality sequences suitable to address taxonomic phylogenetic questions. We tested 13 extraction protocols and two library preparation kits and found the combination of the E.Z.N.A.(®) Forensic DNA kit for DNA extraction and the NuGen Ovation(®) Ultralow System library preparation kit was the most suitable for this purpose.
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spelling pubmed-72197582020-06-01 Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales) Smith, Reannon L. Sawbridge, Tim Mann, Ross Kaur, Jatinder May, Tom W. Edwards, Jacqueline PLoS One Research Article The purpose of this study was to identify a reliable DNA extraction protocol to use on 25-year-old powdery mildew specimens from the reference collection VPRI in order to produce high quality sequences suitable to address taxonomic phylogenetic questions. We tested 13 extraction protocols and two library preparation kits and found the combination of the E.Z.N.A.(®) Forensic DNA kit for DNA extraction and the NuGen Ovation(®) Ultralow System library preparation kit was the most suitable for this purpose. Public Library of Science 2020-05-13 /pmc/articles/PMC7219758/ /pubmed/32401807 http://dx.doi.org/10.1371/journal.pone.0232535 Text en © 2020 Smith et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Smith, Reannon L.
Sawbridge, Tim
Mann, Ross
Kaur, Jatinder
May, Tom W.
Edwards, Jacqueline
Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales)
title Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales)
title_full Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales)
title_fullStr Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales)
title_full_unstemmed Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales)
title_short Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales)
title_sort rediscovering an old foe: optimised molecular methods for dna extraction and sequencing applications for fungarium specimens of powdery mildew (erysiphales)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7219758/
https://www.ncbi.nlm.nih.gov/pubmed/32401807
http://dx.doi.org/10.1371/journal.pone.0232535
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