Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales)

The purpose of this study was to identify a reliable DNA extraction protocol to use on 25-year-old powdery mildew specimens from the reference collection VPRI in order to produce high quality sequences suitable to address taxonomic phylogenetic questions. We tested 13 extraction protocols and two li...

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Detalles Bibliográficos
Autores principales: Smith, Reannon L., Sawbridge, Tim, Mann, Ross, Kaur, Jatinder, May, Tom W., Edwards, Jacqueline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7219758/
https://www.ncbi.nlm.nih.gov/pubmed/32401807
http://dx.doi.org/10.1371/journal.pone.0232535

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