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Topology control of human fibroblast cells monolayer by liquid crystal elastomer
Eukaryotic cells in living tissues form dynamic patterns with spatially varying orientational order that affects important physiological processes such as apoptosis and cell migration. The challenge is how to impart a predesigned map of orientational order onto a growing tissue. Here, we demonstrate...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220327/ https://www.ncbi.nlm.nih.gov/pubmed/32426499 http://dx.doi.org/10.1126/sciadv.aaz6485 |
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author | Turiv, Taras Krieger, Jess Babakhanova, Greta Yu, Hao Shiyanovskii, Sergij V. Wei, Qi-Huo Kim, Min-Ho Lavrentovich, Oleg D. |
author_facet | Turiv, Taras Krieger, Jess Babakhanova, Greta Yu, Hao Shiyanovskii, Sergij V. Wei, Qi-Huo Kim, Min-Ho Lavrentovich, Oleg D. |
author_sort | Turiv, Taras |
collection | PubMed |
description | Eukaryotic cells in living tissues form dynamic patterns with spatially varying orientational order that affects important physiological processes such as apoptosis and cell migration. The challenge is how to impart a predesigned map of orientational order onto a growing tissue. Here, we demonstrate an approach to produce cell monolayers of human dermal fibroblasts with predesigned orientational patterns and topological defects using a photoaligned liquid crystal elastomer (LCE) that swells anisotropically in an aqueous medium. The patterns inscribed into the LCE are replicated by the tissue monolayer and cause a strong spatial variation of cells phenotype, their surface density, and number density fluctuations. Unbinding dynamics of defect pairs intrinsic to active matter is suppressed by anisotropic surface anchoring allowing the estimation of the elastic characteristics of the tissues. The demonstrated patterned LCE approach has potential to control the collective behavior of cells in living tissues, cell differentiation, and tissue morphogenesis. |
format | Online Article Text |
id | pubmed-7220327 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-72203272020-05-18 Topology control of human fibroblast cells monolayer by liquid crystal elastomer Turiv, Taras Krieger, Jess Babakhanova, Greta Yu, Hao Shiyanovskii, Sergij V. Wei, Qi-Huo Kim, Min-Ho Lavrentovich, Oleg D. Sci Adv Research Articles Eukaryotic cells in living tissues form dynamic patterns with spatially varying orientational order that affects important physiological processes such as apoptosis and cell migration. The challenge is how to impart a predesigned map of orientational order onto a growing tissue. Here, we demonstrate an approach to produce cell monolayers of human dermal fibroblasts with predesigned orientational patterns and topological defects using a photoaligned liquid crystal elastomer (LCE) that swells anisotropically in an aqueous medium. The patterns inscribed into the LCE are replicated by the tissue monolayer and cause a strong spatial variation of cells phenotype, their surface density, and number density fluctuations. Unbinding dynamics of defect pairs intrinsic to active matter is suppressed by anisotropic surface anchoring allowing the estimation of the elastic characteristics of the tissues. The demonstrated patterned LCE approach has potential to control the collective behavior of cells in living tissues, cell differentiation, and tissue morphogenesis. American Association for the Advancement of Science 2020-05-13 /pmc/articles/PMC7220327/ /pubmed/32426499 http://dx.doi.org/10.1126/sciadv.aaz6485 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Research Articles Turiv, Taras Krieger, Jess Babakhanova, Greta Yu, Hao Shiyanovskii, Sergij V. Wei, Qi-Huo Kim, Min-Ho Lavrentovich, Oleg D. Topology control of human fibroblast cells monolayer by liquid crystal elastomer |
title | Topology control of human fibroblast cells monolayer by liquid crystal elastomer |
title_full | Topology control of human fibroblast cells monolayer by liquid crystal elastomer |
title_fullStr | Topology control of human fibroblast cells monolayer by liquid crystal elastomer |
title_full_unstemmed | Topology control of human fibroblast cells monolayer by liquid crystal elastomer |
title_short | Topology control of human fibroblast cells monolayer by liquid crystal elastomer |
title_sort | topology control of human fibroblast cells monolayer by liquid crystal elastomer |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220327/ https://www.ncbi.nlm.nih.gov/pubmed/32426499 http://dx.doi.org/10.1126/sciadv.aaz6485 |
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