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Effects of anakinra on the small intestine mucositis induced by methotrexate in rats

Intestinal mucositis is an important problem in the patients receiving cancer treatment. We aimed to investigate the effect of anakinra, which is a well known anti-oxidant and anti-inflammatory agent, on methotrexate-induced small intestine mucositis in rats. Forty rats were divided into 4 groups wi...

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Autores principales: Ozcicek, Fatih, Kara, Ali Veysel, Akbas, Emin Murat, Kurt, Nezahat, Yazici, Gulce Naz, Cankaya, Murat, Mammadov, Renad, Ozcicek, Adalet, Suleyman, Halis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Association for Laboratory Animal Science 2019
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220717/
https://www.ncbi.nlm.nih.gov/pubmed/31787709
http://dx.doi.org/10.1538/expanim.19-0057
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author Ozcicek, Fatih
Kara, Ali Veysel
Akbas, Emin Murat
Kurt, Nezahat
Yazici, Gulce Naz
Cankaya, Murat
Mammadov, Renad
Ozcicek, Adalet
Suleyman, Halis
author_facet Ozcicek, Fatih
Kara, Ali Veysel
Akbas, Emin Murat
Kurt, Nezahat
Yazici, Gulce Naz
Cankaya, Murat
Mammadov, Renad
Ozcicek, Adalet
Suleyman, Halis
author_sort Ozcicek, Fatih
collection PubMed
description Intestinal mucositis is an important problem in the patients receiving cancer treatment. We aimed to investigate the effect of anakinra, which is a well known anti-oxidant and anti-inflammatory agent, on methotrexate-induced small intestine mucositis in rats. Forty rats were divided into 4 groups with 10 in each group. The healthy group (HG) and the methotrexate group (MTXG) were given distilled water, while the methotrexate + anakinra 50 (MTX+ANA50) and the methotrexate + anakinra 100 (MTX+ANA100) groups were intraperitoneally administered 50 and 100 mg/kg of anakinra. After one hour, the MTXG, MTX+ANA50 and MTX+ANA100 groups were given oral methotrexate at a dose of 5 mg/kg. This procedure was repeated once a day for 7 days. After the rats had been sacrificed, the small intestine tissue of rats were removed for the assesment of biochemical markers, histopathological evaluation and gene expression analyze. Statistical analyses of the data were performed using one-way ANOVA. Malondialdehyde (MDA), myeloperoxidase (MPO) and interleukin-6 (IL-6) levels were significantly higher, whereas total glutathione (tGSH) levels were significantly lower in MTXG (P<0.001) compared to other groups. MTX also increased IL-1β and TNF-α gene expression levels in MTXG (P<0.001). Inflammatory cell infiltration and damage to the villus were observed histopathologically in the MTXG group, whereas only mild inflammation was seen in the MTX+ANA100 group. A dose of 100 mg/kg of anakinra prevented the increase of the biochemical markers and gene expression levels better than a dose of 50 mg/kg. Intestinal mucositis caused by MTX may be preventible by co-administered anakinra.
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spelling pubmed-72207172020-05-18 Effects of anakinra on the small intestine mucositis induced by methotrexate in rats Ozcicek, Fatih Kara, Ali Veysel Akbas, Emin Murat Kurt, Nezahat Yazici, Gulce Naz Cankaya, Murat Mammadov, Renad Ozcicek, Adalet Suleyman, Halis Exp Anim Original Intestinal mucositis is an important problem in the patients receiving cancer treatment. We aimed to investigate the effect of anakinra, which is a well known anti-oxidant and anti-inflammatory agent, on methotrexate-induced small intestine mucositis in rats. Forty rats were divided into 4 groups with 10 in each group. The healthy group (HG) and the methotrexate group (MTXG) were given distilled water, while the methotrexate + anakinra 50 (MTX+ANA50) and the methotrexate + anakinra 100 (MTX+ANA100) groups were intraperitoneally administered 50 and 100 mg/kg of anakinra. After one hour, the MTXG, MTX+ANA50 and MTX+ANA100 groups were given oral methotrexate at a dose of 5 mg/kg. This procedure was repeated once a day for 7 days. After the rats had been sacrificed, the small intestine tissue of rats were removed for the assesment of biochemical markers, histopathological evaluation and gene expression analyze. Statistical analyses of the data were performed using one-way ANOVA. Malondialdehyde (MDA), myeloperoxidase (MPO) and interleukin-6 (IL-6) levels were significantly higher, whereas total glutathione (tGSH) levels were significantly lower in MTXG (P<0.001) compared to other groups. MTX also increased IL-1β and TNF-α gene expression levels in MTXG (P<0.001). Inflammatory cell infiltration and damage to the villus were observed histopathologically in the MTXG group, whereas only mild inflammation was seen in the MTX+ANA100 group. A dose of 100 mg/kg of anakinra prevented the increase of the biochemical markers and gene expression levels better than a dose of 50 mg/kg. Intestinal mucositis caused by MTX may be preventible by co-administered anakinra. Japanese Association for Laboratory Animal Science 2019-12-02 2020 /pmc/articles/PMC7220717/ /pubmed/31787709 http://dx.doi.org/10.1538/expanim.19-0057 Text en ©2020 Japanese Association for Laboratory Animal Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Original
Ozcicek, Fatih
Kara, Ali Veysel
Akbas, Emin Murat
Kurt, Nezahat
Yazici, Gulce Naz
Cankaya, Murat
Mammadov, Renad
Ozcicek, Adalet
Suleyman, Halis
Effects of anakinra on the small intestine mucositis induced by methotrexate in rats
title Effects of anakinra on the small intestine mucositis induced by methotrexate in rats
title_full Effects of anakinra on the small intestine mucositis induced by methotrexate in rats
title_fullStr Effects of anakinra on the small intestine mucositis induced by methotrexate in rats
title_full_unstemmed Effects of anakinra on the small intestine mucositis induced by methotrexate in rats
title_short Effects of anakinra on the small intestine mucositis induced by methotrexate in rats
title_sort effects of anakinra on the small intestine mucositis induced by methotrexate in rats
topic Original
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220717/
https://www.ncbi.nlm.nih.gov/pubmed/31787709
http://dx.doi.org/10.1538/expanim.19-0057
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