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Induction of apoptosis by Xiakemycin A in human hepatoma HepG2 cells
Xiakemycin A (XKA), a new antibiotic in the pyranonaphthoquinone family, shows antitumor activity. However, the type of cell death induced by XKA remains elusive. In this study, we aim to investigate the type of death induced by XKA in hepatic cancer. The apoptotic features, such as chromatic agglut...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer Health
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220762/ https://www.ncbi.nlm.nih.gov/pubmed/32332640 http://dx.doi.org/10.1097/MD.0000000000019848 |
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author | Chen, Chuan Han, Zhu Yang, Minjie Jiang, Zhongke Ou, Xiuyuan |
author_facet | Chen, Chuan Han, Zhu Yang, Minjie Jiang, Zhongke Ou, Xiuyuan |
author_sort | Chen, Chuan |
collection | PubMed |
description | Xiakemycin A (XKA), a new antibiotic in the pyranonaphthoquinone family, shows antitumor activity. However, the type of cell death induced by XKA remains elusive. In this study, we aim to investigate the type of death induced by XKA in hepatic cancer. The apoptotic features, such as chromatic agglutination, reactive oxygen species generation and membrane potential of mitochondria, in HepG2 cells treated by XKA were measured by Hoechst 33342 staining and flow cytometry. Apoptosis of HepG2 cells treated with XKA was determined by Annexin V-FITC/propidium iodide double staining and Western blot analysis, respectively. XKA had a significant dose-dependent elevation of chromatic agglutination, reactive oxygen species generation, Annexin V and propidium iodide staining, decrease of membrane potential. Meanwhile, in apoptotic HepG2 cells induced by XKA, robust increment was noticed in p53 expression, cleavage of PARP, caspase-3, and caspase-9. XKA showed potent inhibitory effects on the proliferation of HepG2 cells. Such phenomenon may be related to activation of the apoptotic pathway. |
format | Online Article Text |
id | pubmed-7220762 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Wolters Kluwer Health |
record_format | MEDLINE/PubMed |
spelling | pubmed-72207622020-06-15 Induction of apoptosis by Xiakemycin A in human hepatoma HepG2 cells Chen, Chuan Han, Zhu Yang, Minjie Jiang, Zhongke Ou, Xiuyuan Medicine (Baltimore) 6600 Xiakemycin A (XKA), a new antibiotic in the pyranonaphthoquinone family, shows antitumor activity. However, the type of cell death induced by XKA remains elusive. In this study, we aim to investigate the type of death induced by XKA in hepatic cancer. The apoptotic features, such as chromatic agglutination, reactive oxygen species generation and membrane potential of mitochondria, in HepG2 cells treated by XKA were measured by Hoechst 33342 staining and flow cytometry. Apoptosis of HepG2 cells treated with XKA was determined by Annexin V-FITC/propidium iodide double staining and Western blot analysis, respectively. XKA had a significant dose-dependent elevation of chromatic agglutination, reactive oxygen species generation, Annexin V and propidium iodide staining, decrease of membrane potential. Meanwhile, in apoptotic HepG2 cells induced by XKA, robust increment was noticed in p53 expression, cleavage of PARP, caspase-3, and caspase-9. XKA showed potent inhibitory effects on the proliferation of HepG2 cells. Such phenomenon may be related to activation of the apoptotic pathway. Wolters Kluwer Health 2020-04-24 /pmc/articles/PMC7220762/ /pubmed/32332640 http://dx.doi.org/10.1097/MD.0000000000019848 Text en Copyright © 2020 the Author(s). Published by Wolters Kluwer Health, Inc. http://creativecommons.org/licenses/by-nc/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial License 4.0 (CCBY-NC), where it is permissible to download, share, remix, transform, and buildup the work provided it is properly cited. The work cannot be used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc/4.0 |
spellingShingle | 6600 Chen, Chuan Han, Zhu Yang, Minjie Jiang, Zhongke Ou, Xiuyuan Induction of apoptosis by Xiakemycin A in human hepatoma HepG2 cells |
title | Induction of apoptosis by Xiakemycin A in human hepatoma HepG2 cells |
title_full | Induction of apoptosis by Xiakemycin A in human hepatoma HepG2 cells |
title_fullStr | Induction of apoptosis by Xiakemycin A in human hepatoma HepG2 cells |
title_full_unstemmed | Induction of apoptosis by Xiakemycin A in human hepatoma HepG2 cells |
title_short | Induction of apoptosis by Xiakemycin A in human hepatoma HepG2 cells |
title_sort | induction of apoptosis by xiakemycin a in human hepatoma hepg2 cells |
topic | 6600 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220762/ https://www.ncbi.nlm.nih.gov/pubmed/32332640 http://dx.doi.org/10.1097/MD.0000000000019848 |
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