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Circulating tumour cell enumeration does not correlate with Miller–Payne grade in a cohort of breast cancer patients undergoing neoadjuvant chemotherapy
PURPOSE: The association between pathological complete response (pCR) in patients receiving neoadjuvant chemotherapy (NAC) for breast cancer and Circulating Tumour Cells (CTCs) is not clear. The aim of this study was to assess whether CTC enumeration could be used to predict pathological response to...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220879/ https://www.ncbi.nlm.nih.gov/pubmed/32378053 http://dx.doi.org/10.1007/s10549-020-05658-7 |
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author | O’Toole, Sharon A. Spillane, Cathy Huang, Yanmei Fitzgerald, Marie C. Ffrench, Brendan Mohamed, Bashir Ward, Mark Gallagher, Michael Kelly, Tanya O’Brien, Cathal Ruttle, Carmel Bogdanska, Anna Martin, Cara Mullen, Dorinda Connolly, Elizabeth McGarrigle, Sarah A. Kennedy, John O’Leary, John J. |
author_facet | O’Toole, Sharon A. Spillane, Cathy Huang, Yanmei Fitzgerald, Marie C. Ffrench, Brendan Mohamed, Bashir Ward, Mark Gallagher, Michael Kelly, Tanya O’Brien, Cathal Ruttle, Carmel Bogdanska, Anna Martin, Cara Mullen, Dorinda Connolly, Elizabeth McGarrigle, Sarah A. Kennedy, John O’Leary, John J. |
author_sort | O’Toole, Sharon A. |
collection | PubMed |
description | PURPOSE: The association between pathological complete response (pCR) in patients receiving neoadjuvant chemotherapy (NAC) for breast cancer and Circulating Tumour Cells (CTCs) is not clear. The aim of this study was to assess whether CTC enumeration could be used to predict pathological response to NAC in breast cancer as measured by the Miller–Payne grading system. METHODS: Twenty-six patients were recruited, and blood samples were taken pre- and post-NAC. CTCs were isolated using the ScreenCell device and stained using a modified Giemsa stain. CTCs were enumerated by 2 pathologists and classified as single CTCs, doublets, clusters/microemboli and correlated with the pathological response as measured by the Miller–Payne grading system. χ(2) or ANOVA was performed in SPSS 24.0 statistics software for associations. RESULTS: 89% of patients had invasive ductal carcinoma (IDC) and 11% invasive lobular carcinoma (ILC). At baseline 85% of patients had CTCs present, median 7 (0–161) CTCs per 3 ml of whole blood. Post-chemotherapy, 58% had an increase in CTCs. This did not correlate with the Miller–Payne grade of response. No significant association was identified between the number of CTCs and clinical characteristics; however, we did observe a correlation between pre-treatment CTC counts and body mass index, p < 0.05. CONCLUSIONS: Patients with a complete response to NAC still had CTCs present, suggesting enumeration is not sufficient to aid surgery stratification. Additional characterisation and larger studies are needed to further characterise CTCs isolated pre- and post-chemotherapy. Long-term follow-up of these patients will determine the significance of CTCs in NAC breast cancer patients. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10549-020-05658-7) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-7220879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-72208792020-05-14 Circulating tumour cell enumeration does not correlate with Miller–Payne grade in a cohort of breast cancer patients undergoing neoadjuvant chemotherapy O’Toole, Sharon A. Spillane, Cathy Huang, Yanmei Fitzgerald, Marie C. Ffrench, Brendan Mohamed, Bashir Ward, Mark Gallagher, Michael Kelly, Tanya O’Brien, Cathal Ruttle, Carmel Bogdanska, Anna Martin, Cara Mullen, Dorinda Connolly, Elizabeth McGarrigle, Sarah A. Kennedy, John O’Leary, John J. Breast Cancer Res Treat Preclinical Study PURPOSE: The association between pathological complete response (pCR) in patients receiving neoadjuvant chemotherapy (NAC) for breast cancer and Circulating Tumour Cells (CTCs) is not clear. The aim of this study was to assess whether CTC enumeration could be used to predict pathological response to NAC in breast cancer as measured by the Miller–Payne grading system. METHODS: Twenty-six patients were recruited, and blood samples were taken pre- and post-NAC. CTCs were isolated using the ScreenCell device and stained using a modified Giemsa stain. CTCs were enumerated by 2 pathologists and classified as single CTCs, doublets, clusters/microemboli and correlated with the pathological response as measured by the Miller–Payne grading system. χ(2) or ANOVA was performed in SPSS 24.0 statistics software for associations. RESULTS: 89% of patients had invasive ductal carcinoma (IDC) and 11% invasive lobular carcinoma (ILC). At baseline 85% of patients had CTCs present, median 7 (0–161) CTCs per 3 ml of whole blood. Post-chemotherapy, 58% had an increase in CTCs. This did not correlate with the Miller–Payne grade of response. No significant association was identified between the number of CTCs and clinical characteristics; however, we did observe a correlation between pre-treatment CTC counts and body mass index, p < 0.05. CONCLUSIONS: Patients with a complete response to NAC still had CTCs present, suggesting enumeration is not sufficient to aid surgery stratification. Additional characterisation and larger studies are needed to further characterise CTCs isolated pre- and post-chemotherapy. Long-term follow-up of these patients will determine the significance of CTCs in NAC breast cancer patients. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10549-020-05658-7) contains supplementary material, which is available to authorized users. Springer US 2020-05-06 2020 /pmc/articles/PMC7220879/ /pubmed/32378053 http://dx.doi.org/10.1007/s10549-020-05658-7 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Preclinical Study O’Toole, Sharon A. Spillane, Cathy Huang, Yanmei Fitzgerald, Marie C. Ffrench, Brendan Mohamed, Bashir Ward, Mark Gallagher, Michael Kelly, Tanya O’Brien, Cathal Ruttle, Carmel Bogdanska, Anna Martin, Cara Mullen, Dorinda Connolly, Elizabeth McGarrigle, Sarah A. Kennedy, John O’Leary, John J. Circulating tumour cell enumeration does not correlate with Miller–Payne grade in a cohort of breast cancer patients undergoing neoadjuvant chemotherapy |
title | Circulating tumour cell enumeration does not correlate with Miller–Payne grade in a cohort of breast cancer patients undergoing neoadjuvant chemotherapy |
title_full | Circulating tumour cell enumeration does not correlate with Miller–Payne grade in a cohort of breast cancer patients undergoing neoadjuvant chemotherapy |
title_fullStr | Circulating tumour cell enumeration does not correlate with Miller–Payne grade in a cohort of breast cancer patients undergoing neoadjuvant chemotherapy |
title_full_unstemmed | Circulating tumour cell enumeration does not correlate with Miller–Payne grade in a cohort of breast cancer patients undergoing neoadjuvant chemotherapy |
title_short | Circulating tumour cell enumeration does not correlate with Miller–Payne grade in a cohort of breast cancer patients undergoing neoadjuvant chemotherapy |
title_sort | circulating tumour cell enumeration does not correlate with miller–payne grade in a cohort of breast cancer patients undergoing neoadjuvant chemotherapy |
topic | Preclinical Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220879/ https://www.ncbi.nlm.nih.gov/pubmed/32378053 http://dx.doi.org/10.1007/s10549-020-05658-7 |
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