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Target-responsive vasoactive probes for ultrasensitive molecular imaging
The ability to monitor molecules volumetrically throughout the body could provide valuable biomarkers for studies of healthy function and disease, but noninvasive detection of molecular targets in living subjects often suffers from poor sensitivity or selectivity. Here we describe a family of potent...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220906/ https://www.ncbi.nlm.nih.gov/pubmed/32404879 http://dx.doi.org/10.1038/s41467-020-16118-7 |
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author | Ohlendorf, Robert Wiśniowska, Agata Desai, Mitul Barandov, Ali Slusarczyk, Adrian L. Li, Nan Jasanoff, Alan |
author_facet | Ohlendorf, Robert Wiśniowska, Agata Desai, Mitul Barandov, Ali Slusarczyk, Adrian L. Li, Nan Jasanoff, Alan |
author_sort | Ohlendorf, Robert |
collection | PubMed |
description | The ability to monitor molecules volumetrically throughout the body could provide valuable biomarkers for studies of healthy function and disease, but noninvasive detection of molecular targets in living subjects often suffers from poor sensitivity or selectivity. Here we describe a family of potent imaging probes that can be activated by molecules of interest in deep tissue, providing a basis for mapping nanomolar-scale analytes without the radiation or heavy metal content associated with traditional molecular imaging agents. The probes are reversibly caged vasodilators that induce responses detectable by hemodynamic imaging; they are constructed by combining vasoactive peptides with synthetic chemical appendages and protein blocking domains. We use this architecture to create ultrasensitive biotin-responsive imaging agents, which we apply for wide-field mapping of targets in rat brains using functional magnetic resonance imaging. We also adapt the sensor design for detecting the neurotransmitter dopamine, illustrating versatility of this approach for addressing biologically important molecules. |
format | Online Article Text |
id | pubmed-7220906 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-72209062020-05-15 Target-responsive vasoactive probes for ultrasensitive molecular imaging Ohlendorf, Robert Wiśniowska, Agata Desai, Mitul Barandov, Ali Slusarczyk, Adrian L. Li, Nan Jasanoff, Alan Nat Commun Article The ability to monitor molecules volumetrically throughout the body could provide valuable biomarkers for studies of healthy function and disease, but noninvasive detection of molecular targets in living subjects often suffers from poor sensitivity or selectivity. Here we describe a family of potent imaging probes that can be activated by molecules of interest in deep tissue, providing a basis for mapping nanomolar-scale analytes without the radiation or heavy metal content associated with traditional molecular imaging agents. The probes are reversibly caged vasodilators that induce responses detectable by hemodynamic imaging; they are constructed by combining vasoactive peptides with synthetic chemical appendages and protein blocking domains. We use this architecture to create ultrasensitive biotin-responsive imaging agents, which we apply for wide-field mapping of targets in rat brains using functional magnetic resonance imaging. We also adapt the sensor design for detecting the neurotransmitter dopamine, illustrating versatility of this approach for addressing biologically important molecules. Nature Publishing Group UK 2020-05-13 /pmc/articles/PMC7220906/ /pubmed/32404879 http://dx.doi.org/10.1038/s41467-020-16118-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ohlendorf, Robert Wiśniowska, Agata Desai, Mitul Barandov, Ali Slusarczyk, Adrian L. Li, Nan Jasanoff, Alan Target-responsive vasoactive probes for ultrasensitive molecular imaging |
title | Target-responsive vasoactive probes for ultrasensitive molecular imaging |
title_full | Target-responsive vasoactive probes for ultrasensitive molecular imaging |
title_fullStr | Target-responsive vasoactive probes for ultrasensitive molecular imaging |
title_full_unstemmed | Target-responsive vasoactive probes for ultrasensitive molecular imaging |
title_short | Target-responsive vasoactive probes for ultrasensitive molecular imaging |
title_sort | target-responsive vasoactive probes for ultrasensitive molecular imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7220906/ https://www.ncbi.nlm.nih.gov/pubmed/32404879 http://dx.doi.org/10.1038/s41467-020-16118-7 |
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