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High-Efficiency Secretion and Directed Evolution of Chitinase BcChiA1 in Bacillus subtilis for the Conversion of Chitinaceous Wastes Into Chitooligosaccharides
Limitations of enzyme production and activity pose a challenge for efficient degradation of chitinaceous wastes. To solve this problem, we engineered a system for high-yielding extracellular secretion of chitinase A1 from Bacillus circulans (BcChiA1) in B. subtilis. Furthermore, an innovative chitin...
Autores principales: | Wang, Sijia, Fu, Gang, Li, Jinlong, Wei, Xunfan, Fang, Huan, Huang, Dawei, Lin, Jianping, Zhang, Dawei |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221128/ https://www.ncbi.nlm.nih.gov/pubmed/32457893 http://dx.doi.org/10.3389/fbioe.2020.00432 |
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