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Biological Safety and Biodistribution of Chitosan Nanoparticles

The effect of unmodified chitosan nanoparticles with a size of ~100 nm and a weakly positive charge on blood coagulation, metabolic activity of cultured cardiomyocytes, general toxicity, biodistribution, and reactive changes in rat organs in response to their single intravenous administration at dos...

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Autores principales: Sonin, Dmitry, Pochkaeva, Evgeniia, Zhuravskii, Sergei, Postnov, Viktor, Korolev, Dmitry, Vasina, Lyubov, Kostina, Daria, Mukhametdinova, Daria, Zelinskaya, Irina, Skorik, Yury, Naumysheva, Elena, Malashicheva, Anna, Somov, Pavel, Istomina, Maria, Rubanova, Natalia, Aleksandrov, Ilia, Vasyutina, Marina, Galagudza, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221586/
https://www.ncbi.nlm.nih.gov/pubmed/32340313
http://dx.doi.org/10.3390/nano10040810
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author Sonin, Dmitry
Pochkaeva, Evgeniia
Zhuravskii, Sergei
Postnov, Viktor
Korolev, Dmitry
Vasina, Lyubov
Kostina, Daria
Mukhametdinova, Daria
Zelinskaya, Irina
Skorik, Yury
Naumysheva, Elena
Malashicheva, Anna
Somov, Pavel
Istomina, Maria
Rubanova, Natalia
Aleksandrov, Ilia
Vasyutina, Marina
Galagudza, Michael
author_facet Sonin, Dmitry
Pochkaeva, Evgeniia
Zhuravskii, Sergei
Postnov, Viktor
Korolev, Dmitry
Vasina, Lyubov
Kostina, Daria
Mukhametdinova, Daria
Zelinskaya, Irina
Skorik, Yury
Naumysheva, Elena
Malashicheva, Anna
Somov, Pavel
Istomina, Maria
Rubanova, Natalia
Aleksandrov, Ilia
Vasyutina, Marina
Galagudza, Michael
author_sort Sonin, Dmitry
collection PubMed
description The effect of unmodified chitosan nanoparticles with a size of ~100 nm and a weakly positive charge on blood coagulation, metabolic activity of cultured cardiomyocytes, general toxicity, biodistribution, and reactive changes in rat organs in response to their single intravenous administration at doses of 1, 2, and 4 mg/kg was studied. Chitosan nanoparticles (CNPs) have a small cytotoxic effect and have a weak antiplatelet and anticoagulant effect. Intravenous administration of CNPs does not cause significant hemodynamic changes, and 30 min after the CNPs administration, they mainly accumulate in the liver and lungs, without causing hemolysis and leukocytosis. The toxicity of chitosan nanoparticles was manifested in a dose-dependent short-term delay in weight gain with subsequent recovery, while in the 2-week observation period no signs of pain and distress were observed in rats. Granulomas found in the lungs and liver indicate slow biodegradation of chitosan nanoparticles. In general, the obtained results indicate a good tolerance of intravenous administration of an unmodified chitosan suspension in the studied dose range.
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spelling pubmed-72215862020-05-22 Biological Safety and Biodistribution of Chitosan Nanoparticles Sonin, Dmitry Pochkaeva, Evgeniia Zhuravskii, Sergei Postnov, Viktor Korolev, Dmitry Vasina, Lyubov Kostina, Daria Mukhametdinova, Daria Zelinskaya, Irina Skorik, Yury Naumysheva, Elena Malashicheva, Anna Somov, Pavel Istomina, Maria Rubanova, Natalia Aleksandrov, Ilia Vasyutina, Marina Galagudza, Michael Nanomaterials (Basel) Article The effect of unmodified chitosan nanoparticles with a size of ~100 nm and a weakly positive charge on blood coagulation, metabolic activity of cultured cardiomyocytes, general toxicity, biodistribution, and reactive changes in rat organs in response to their single intravenous administration at doses of 1, 2, and 4 mg/kg was studied. Chitosan nanoparticles (CNPs) have a small cytotoxic effect and have a weak antiplatelet and anticoagulant effect. Intravenous administration of CNPs does not cause significant hemodynamic changes, and 30 min after the CNPs administration, they mainly accumulate in the liver and lungs, without causing hemolysis and leukocytosis. The toxicity of chitosan nanoparticles was manifested in a dose-dependent short-term delay in weight gain with subsequent recovery, while in the 2-week observation period no signs of pain and distress were observed in rats. Granulomas found in the lungs and liver indicate slow biodegradation of chitosan nanoparticles. In general, the obtained results indicate a good tolerance of intravenous administration of an unmodified chitosan suspension in the studied dose range. MDPI 2020-04-23 /pmc/articles/PMC7221586/ /pubmed/32340313 http://dx.doi.org/10.3390/nano10040810 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sonin, Dmitry
Pochkaeva, Evgeniia
Zhuravskii, Sergei
Postnov, Viktor
Korolev, Dmitry
Vasina, Lyubov
Kostina, Daria
Mukhametdinova, Daria
Zelinskaya, Irina
Skorik, Yury
Naumysheva, Elena
Malashicheva, Anna
Somov, Pavel
Istomina, Maria
Rubanova, Natalia
Aleksandrov, Ilia
Vasyutina, Marina
Galagudza, Michael
Biological Safety and Biodistribution of Chitosan Nanoparticles
title Biological Safety and Biodistribution of Chitosan Nanoparticles
title_full Biological Safety and Biodistribution of Chitosan Nanoparticles
title_fullStr Biological Safety and Biodistribution of Chitosan Nanoparticles
title_full_unstemmed Biological Safety and Biodistribution of Chitosan Nanoparticles
title_short Biological Safety and Biodistribution of Chitosan Nanoparticles
title_sort biological safety and biodistribution of chitosan nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221586/
https://www.ncbi.nlm.nih.gov/pubmed/32340313
http://dx.doi.org/10.3390/nano10040810
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