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A Higher Frequency Administration of the Nontoxic Cycloartane-Type Triterpene Argentatin A Improved Its Anti-Tumor Activity

Parthenium argentatum (Gray), commonly known as guayule, has been used to obtain natural rubber since the beginning of the 20th century. Additionally, the so called “resin” is a waste product derived from the industrial process. The cycloartane-type triterpene Argentatin A (AA) is one of the main co...

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Autores principales: Tavarez-Santamaría, Zaira, Jacobo-Herrera, Nadia J., Rocha-Zavaleta, Leticia, Zentella-Dehesa, Alejandro, Couder-García, Beatriz del Carmen, Martínez-Vázquez, Mariano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221627/
https://www.ncbi.nlm.nih.gov/pubmed/32295227
http://dx.doi.org/10.3390/molecules25081780
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author Tavarez-Santamaría, Zaira
Jacobo-Herrera, Nadia J.
Rocha-Zavaleta, Leticia
Zentella-Dehesa, Alejandro
Couder-García, Beatriz del Carmen
Martínez-Vázquez, Mariano
author_facet Tavarez-Santamaría, Zaira
Jacobo-Herrera, Nadia J.
Rocha-Zavaleta, Leticia
Zentella-Dehesa, Alejandro
Couder-García, Beatriz del Carmen
Martínez-Vázquez, Mariano
author_sort Tavarez-Santamaría, Zaira
collection PubMed
description Parthenium argentatum (Gray), commonly known as guayule, has been used to obtain natural rubber since the beginning of the 20th century. Additionally, the so called “resin” is a waste product derived from the industrial process. The cycloartane-type triterpene Argentatin A (AA) is one of the main constituents of the industrial waste resin. In this study we evaluated the AA anticancer activity both in vitro and in vivo in the HCT116 colon cancer cells. The apoptosis promotion of AA was assessed by the annexin V/propidium iodide (PI) assay. The senescence was evaluated for SA-β-galactosidase, and PCNA was used as a marker of proliferation. Its antitumor activity was evaluated using a xenograft mouse model. The results indicated that AA-induced apoptosis in HCT-116 cells and was positively stained for SA-β-galactosidase. In the xenografted mice test, the administration of AA at the dose of 250 mg/kg three times a week for 21 days reduced tumor growth by 78.1%. A comparable tumor reduction was achieved with cisplatin at the dose of 2 mg/kg administered three times a week for 21 days. However, nude mice treated with AA did not lose weight, as they did remarkably when treated with cisplatin. Furthermore, the animals treated with AA showed similar blood profiles as the healthy control group. These data indicate the low toxicity of AA compared to that shown by cisplatin.
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spelling pubmed-72216272020-05-22 A Higher Frequency Administration of the Nontoxic Cycloartane-Type Triterpene Argentatin A Improved Its Anti-Tumor Activity Tavarez-Santamaría, Zaira Jacobo-Herrera, Nadia J. Rocha-Zavaleta, Leticia Zentella-Dehesa, Alejandro Couder-García, Beatriz del Carmen Martínez-Vázquez, Mariano Molecules Article Parthenium argentatum (Gray), commonly known as guayule, has been used to obtain natural rubber since the beginning of the 20th century. Additionally, the so called “resin” is a waste product derived from the industrial process. The cycloartane-type triterpene Argentatin A (AA) is one of the main constituents of the industrial waste resin. In this study we evaluated the AA anticancer activity both in vitro and in vivo in the HCT116 colon cancer cells. The apoptosis promotion of AA was assessed by the annexin V/propidium iodide (PI) assay. The senescence was evaluated for SA-β-galactosidase, and PCNA was used as a marker of proliferation. Its antitumor activity was evaluated using a xenograft mouse model. The results indicated that AA-induced apoptosis in HCT-116 cells and was positively stained for SA-β-galactosidase. In the xenografted mice test, the administration of AA at the dose of 250 mg/kg three times a week for 21 days reduced tumor growth by 78.1%. A comparable tumor reduction was achieved with cisplatin at the dose of 2 mg/kg administered three times a week for 21 days. However, nude mice treated with AA did not lose weight, as they did remarkably when treated with cisplatin. Furthermore, the animals treated with AA showed similar blood profiles as the healthy control group. These data indicate the low toxicity of AA compared to that shown by cisplatin. MDPI 2020-04-14 /pmc/articles/PMC7221627/ /pubmed/32295227 http://dx.doi.org/10.3390/molecules25081780 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tavarez-Santamaría, Zaira
Jacobo-Herrera, Nadia J.
Rocha-Zavaleta, Leticia
Zentella-Dehesa, Alejandro
Couder-García, Beatriz del Carmen
Martínez-Vázquez, Mariano
A Higher Frequency Administration of the Nontoxic Cycloartane-Type Triterpene Argentatin A Improved Its Anti-Tumor Activity
title A Higher Frequency Administration of the Nontoxic Cycloartane-Type Triterpene Argentatin A Improved Its Anti-Tumor Activity
title_full A Higher Frequency Administration of the Nontoxic Cycloartane-Type Triterpene Argentatin A Improved Its Anti-Tumor Activity
title_fullStr A Higher Frequency Administration of the Nontoxic Cycloartane-Type Triterpene Argentatin A Improved Its Anti-Tumor Activity
title_full_unstemmed A Higher Frequency Administration of the Nontoxic Cycloartane-Type Triterpene Argentatin A Improved Its Anti-Tumor Activity
title_short A Higher Frequency Administration of the Nontoxic Cycloartane-Type Triterpene Argentatin A Improved Its Anti-Tumor Activity
title_sort higher frequency administration of the nontoxic cycloartane-type triterpene argentatin a improved its anti-tumor activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221627/
https://www.ncbi.nlm.nih.gov/pubmed/32295227
http://dx.doi.org/10.3390/molecules25081780
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