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Magnetron-Sputtered Polytetrafluoroethylene-Stabilized Silver Nanoisland Surface for Surface-Enhanced Fluorescence

Surface-enhanced fluorescence (SEF) requires the absorption/emission band of the fluorophore, the localized surface plasmon resonance (LSPR) of the nanostructure and the excitation wavelength to fall in the same (or very close) spectral range. In this paper, we monitor the SEF intensity and lifetime...

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Autores principales: Šubr, Martin, Praus, Petr, Kuzminova, Anna, Kočišová, Eva, Kylián, Ondřej, Sureau, Franck, Procházka, Marek, Štěpánek, Josef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221687/
https://www.ncbi.nlm.nih.gov/pubmed/32316367
http://dx.doi.org/10.3390/nano10040773
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author Šubr, Martin
Praus, Petr
Kuzminova, Anna
Kočišová, Eva
Kylián, Ondřej
Sureau, Franck
Procházka, Marek
Štěpánek, Josef
author_facet Šubr, Martin
Praus, Petr
Kuzminova, Anna
Kočišová, Eva
Kylián, Ondřej
Sureau, Franck
Procházka, Marek
Štěpánek, Josef
author_sort Šubr, Martin
collection PubMed
description Surface-enhanced fluorescence (SEF) requires the absorption/emission band of the fluorophore, the localized surface plasmon resonance (LSPR) of the nanostructure and the excitation wavelength to fall in the same (or very close) spectral range. In this paper, we monitor the SEF intensity and lifetime dependence of riboflavin (vitamin B2) adsorbed on a spacer-modified Ag substrate with respect to the thickness of the spacer. The substrates were formed by silver nanoislands deposited onto magnetron-sputtered polytetrafluoroethylene (ms-PTFE). The spacer was formed by the ms-PTFE layer with the thickness ranging from ~5 to 25 nm. The riboflavin dissolved in dimethylsulfoxide (DMSO) at a 10 µM concentration forms, at the ms-PTFE surface, a homogeneous layer of adsorbed molecules corresponding to a monomolecular layer. The microspectroscopic measurements of the adsorbed layer were performed through a sessile droplet; our study has shown the advantages and limitations of this approach. Time-resolved fluorescence enabled us to determine the enhanced fluorescence quantum yield due to the shortening of the radiative decay in the vicinity of the plasmonic surface. For the 5 nm ms-PTFE layer possessing the largest (estimated 4×) fluorescence enhancement, the quantum yield was increased 2.3×.
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spelling pubmed-72216872020-05-21 Magnetron-Sputtered Polytetrafluoroethylene-Stabilized Silver Nanoisland Surface for Surface-Enhanced Fluorescence Šubr, Martin Praus, Petr Kuzminova, Anna Kočišová, Eva Kylián, Ondřej Sureau, Franck Procházka, Marek Štěpánek, Josef Nanomaterials (Basel) Article Surface-enhanced fluorescence (SEF) requires the absorption/emission band of the fluorophore, the localized surface plasmon resonance (LSPR) of the nanostructure and the excitation wavelength to fall in the same (or very close) spectral range. In this paper, we monitor the SEF intensity and lifetime dependence of riboflavin (vitamin B2) adsorbed on a spacer-modified Ag substrate with respect to the thickness of the spacer. The substrates were formed by silver nanoislands deposited onto magnetron-sputtered polytetrafluoroethylene (ms-PTFE). The spacer was formed by the ms-PTFE layer with the thickness ranging from ~5 to 25 nm. The riboflavin dissolved in dimethylsulfoxide (DMSO) at a 10 µM concentration forms, at the ms-PTFE surface, a homogeneous layer of adsorbed molecules corresponding to a monomolecular layer. The microspectroscopic measurements of the adsorbed layer were performed through a sessile droplet; our study has shown the advantages and limitations of this approach. Time-resolved fluorescence enabled us to determine the enhanced fluorescence quantum yield due to the shortening of the radiative decay in the vicinity of the plasmonic surface. For the 5 nm ms-PTFE layer possessing the largest (estimated 4×) fluorescence enhancement, the quantum yield was increased 2.3×. MDPI 2020-04-16 /pmc/articles/PMC7221687/ /pubmed/32316367 http://dx.doi.org/10.3390/nano10040773 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Šubr, Martin
Praus, Petr
Kuzminova, Anna
Kočišová, Eva
Kylián, Ondřej
Sureau, Franck
Procházka, Marek
Štěpánek, Josef
Magnetron-Sputtered Polytetrafluoroethylene-Stabilized Silver Nanoisland Surface for Surface-Enhanced Fluorescence
title Magnetron-Sputtered Polytetrafluoroethylene-Stabilized Silver Nanoisland Surface for Surface-Enhanced Fluorescence
title_full Magnetron-Sputtered Polytetrafluoroethylene-Stabilized Silver Nanoisland Surface for Surface-Enhanced Fluorescence
title_fullStr Magnetron-Sputtered Polytetrafluoroethylene-Stabilized Silver Nanoisland Surface for Surface-Enhanced Fluorescence
title_full_unstemmed Magnetron-Sputtered Polytetrafluoroethylene-Stabilized Silver Nanoisland Surface for Surface-Enhanced Fluorescence
title_short Magnetron-Sputtered Polytetrafluoroethylene-Stabilized Silver Nanoisland Surface for Surface-Enhanced Fluorescence
title_sort magnetron-sputtered polytetrafluoroethylene-stabilized silver nanoisland surface for surface-enhanced fluorescence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221687/
https://www.ncbi.nlm.nih.gov/pubmed/32316367
http://dx.doi.org/10.3390/nano10040773
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