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Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study

Background: Titanium implant surfaces are continuously modified to improve biocompatibility and to promote osteointegration. Graphene oxide (GO) has been successfully used to ameliorate biomaterial performances, in terms of implant integration with host tissue. The aim of this study is to evaluate t...

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Autores principales: Di Carlo, Roberta, Di Crescenzo, Antonello, Pilato, Serena, Ventrella, Alessia, Piattelli, Adriano, Recinella, Lucia, Chiavaroli, Annalisa, Giordani, Silvia, Baldrighi, Michele, Camisasca, Adalberto, Zavan, Barbara, Falconi, Mirella, Cataldi, Amelia, Fontana, Antonella, Zara, Susi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221922/
https://www.ncbi.nlm.nih.gov/pubmed/32244572
http://dx.doi.org/10.3390/nano10040654
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author Di Carlo, Roberta
Di Crescenzo, Antonello
Pilato, Serena
Ventrella, Alessia
Piattelli, Adriano
Recinella, Lucia
Chiavaroli, Annalisa
Giordani, Silvia
Baldrighi, Michele
Camisasca, Adalberto
Zavan, Barbara
Falconi, Mirella
Cataldi, Amelia
Fontana, Antonella
Zara, Susi
author_facet Di Carlo, Roberta
Di Crescenzo, Antonello
Pilato, Serena
Ventrella, Alessia
Piattelli, Adriano
Recinella, Lucia
Chiavaroli, Annalisa
Giordani, Silvia
Baldrighi, Michele
Camisasca, Adalberto
Zavan, Barbara
Falconi, Mirella
Cataldi, Amelia
Fontana, Antonella
Zara, Susi
author_sort Di Carlo, Roberta
collection PubMed
description Background: Titanium implant surfaces are continuously modified to improve biocompatibility and to promote osteointegration. Graphene oxide (GO) has been successfully used to ameliorate biomaterial performances, in terms of implant integration with host tissue. The aim of this study is to evaluate the Dental Pulp Stem Cells (DPSCs) viability, cytotoxic response, and osteogenic differentiation capability in the presence of GO-coated titanium surfaces. Methods: Two titanium discs types, machined (control, Crtl) and sandblasted and acid-etched (test, Test) discs, were covalently functionalized with GO. The ability of the GO-functionalized substrates to allow the proliferation and differentiation of DPSCs, as well as their cytotoxic potential, were assessed. Results: The functionalization procedures provide a homogeneous coating with GO of the titanium surface in both control and test substrates, with unchanged surface roughness with respect to the untreated surfaces. All samples show the deposition of extracellular matrix, more pronounced in the test and GO-functionalized test discs. GO-functionalized test samples evidenced a significant viability, with no cytotoxic response and a remarkable early stage proliferation of DPSCs cells, followed by their successful differentiation into osteoblasts. Conclusions: The described protocol of GO-functionalization provides a novel not cytotoxic biomaterial that is able to stimulate cell viability and that better and more quickly induces osteogenic differentiation with respect to simple titanium discs. Our findings pave the way to exploit this GO-functionalization protocol for the production of novel dental implant materials that display improved integration with the host tissue.
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spelling pubmed-72219222020-05-22 Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study Di Carlo, Roberta Di Crescenzo, Antonello Pilato, Serena Ventrella, Alessia Piattelli, Adriano Recinella, Lucia Chiavaroli, Annalisa Giordani, Silvia Baldrighi, Michele Camisasca, Adalberto Zavan, Barbara Falconi, Mirella Cataldi, Amelia Fontana, Antonella Zara, Susi Nanomaterials (Basel) Article Background: Titanium implant surfaces are continuously modified to improve biocompatibility and to promote osteointegration. Graphene oxide (GO) has been successfully used to ameliorate biomaterial performances, in terms of implant integration with host tissue. The aim of this study is to evaluate the Dental Pulp Stem Cells (DPSCs) viability, cytotoxic response, and osteogenic differentiation capability in the presence of GO-coated titanium surfaces. Methods: Two titanium discs types, machined (control, Crtl) and sandblasted and acid-etched (test, Test) discs, were covalently functionalized with GO. The ability of the GO-functionalized substrates to allow the proliferation and differentiation of DPSCs, as well as their cytotoxic potential, were assessed. Results: The functionalization procedures provide a homogeneous coating with GO of the titanium surface in both control and test substrates, with unchanged surface roughness with respect to the untreated surfaces. All samples show the deposition of extracellular matrix, more pronounced in the test and GO-functionalized test discs. GO-functionalized test samples evidenced a significant viability, with no cytotoxic response and a remarkable early stage proliferation of DPSCs cells, followed by their successful differentiation into osteoblasts. Conclusions: The described protocol of GO-functionalization provides a novel not cytotoxic biomaterial that is able to stimulate cell viability and that better and more quickly induces osteogenic differentiation with respect to simple titanium discs. Our findings pave the way to exploit this GO-functionalization protocol for the production of novel dental implant materials that display improved integration with the host tissue. MDPI 2020-04-01 /pmc/articles/PMC7221922/ /pubmed/32244572 http://dx.doi.org/10.3390/nano10040654 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Di Carlo, Roberta
Di Crescenzo, Antonello
Pilato, Serena
Ventrella, Alessia
Piattelli, Adriano
Recinella, Lucia
Chiavaroli, Annalisa
Giordani, Silvia
Baldrighi, Michele
Camisasca, Adalberto
Zavan, Barbara
Falconi, Mirella
Cataldi, Amelia
Fontana, Antonella
Zara, Susi
Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study
title Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study
title_full Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study
title_fullStr Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study
title_full_unstemmed Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study
title_short Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study
title_sort osteoblastic differentiation on graphene oxide-functionalized titanium surfaces: an in vitro study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221922/
https://www.ncbi.nlm.nih.gov/pubmed/32244572
http://dx.doi.org/10.3390/nano10040654
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