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Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study
Background: Titanium implant surfaces are continuously modified to improve biocompatibility and to promote osteointegration. Graphene oxide (GO) has been successfully used to ameliorate biomaterial performances, in terms of implant integration with host tissue. The aim of this study is to evaluate t...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221922/ https://www.ncbi.nlm.nih.gov/pubmed/32244572 http://dx.doi.org/10.3390/nano10040654 |
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author | Di Carlo, Roberta Di Crescenzo, Antonello Pilato, Serena Ventrella, Alessia Piattelli, Adriano Recinella, Lucia Chiavaroli, Annalisa Giordani, Silvia Baldrighi, Michele Camisasca, Adalberto Zavan, Barbara Falconi, Mirella Cataldi, Amelia Fontana, Antonella Zara, Susi |
author_facet | Di Carlo, Roberta Di Crescenzo, Antonello Pilato, Serena Ventrella, Alessia Piattelli, Adriano Recinella, Lucia Chiavaroli, Annalisa Giordani, Silvia Baldrighi, Michele Camisasca, Adalberto Zavan, Barbara Falconi, Mirella Cataldi, Amelia Fontana, Antonella Zara, Susi |
author_sort | Di Carlo, Roberta |
collection | PubMed |
description | Background: Titanium implant surfaces are continuously modified to improve biocompatibility and to promote osteointegration. Graphene oxide (GO) has been successfully used to ameliorate biomaterial performances, in terms of implant integration with host tissue. The aim of this study is to evaluate the Dental Pulp Stem Cells (DPSCs) viability, cytotoxic response, and osteogenic differentiation capability in the presence of GO-coated titanium surfaces. Methods: Two titanium discs types, machined (control, Crtl) and sandblasted and acid-etched (test, Test) discs, were covalently functionalized with GO. The ability of the GO-functionalized substrates to allow the proliferation and differentiation of DPSCs, as well as their cytotoxic potential, were assessed. Results: The functionalization procedures provide a homogeneous coating with GO of the titanium surface in both control and test substrates, with unchanged surface roughness with respect to the untreated surfaces. All samples show the deposition of extracellular matrix, more pronounced in the test and GO-functionalized test discs. GO-functionalized test samples evidenced a significant viability, with no cytotoxic response and a remarkable early stage proliferation of DPSCs cells, followed by their successful differentiation into osteoblasts. Conclusions: The described protocol of GO-functionalization provides a novel not cytotoxic biomaterial that is able to stimulate cell viability and that better and more quickly induces osteogenic differentiation with respect to simple titanium discs. Our findings pave the way to exploit this GO-functionalization protocol for the production of novel dental implant materials that display improved integration with the host tissue. |
format | Online Article Text |
id | pubmed-7221922 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72219222020-05-22 Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study Di Carlo, Roberta Di Crescenzo, Antonello Pilato, Serena Ventrella, Alessia Piattelli, Adriano Recinella, Lucia Chiavaroli, Annalisa Giordani, Silvia Baldrighi, Michele Camisasca, Adalberto Zavan, Barbara Falconi, Mirella Cataldi, Amelia Fontana, Antonella Zara, Susi Nanomaterials (Basel) Article Background: Titanium implant surfaces are continuously modified to improve biocompatibility and to promote osteointegration. Graphene oxide (GO) has been successfully used to ameliorate biomaterial performances, in terms of implant integration with host tissue. The aim of this study is to evaluate the Dental Pulp Stem Cells (DPSCs) viability, cytotoxic response, and osteogenic differentiation capability in the presence of GO-coated titanium surfaces. Methods: Two titanium discs types, machined (control, Crtl) and sandblasted and acid-etched (test, Test) discs, were covalently functionalized with GO. The ability of the GO-functionalized substrates to allow the proliferation and differentiation of DPSCs, as well as their cytotoxic potential, were assessed. Results: The functionalization procedures provide a homogeneous coating with GO of the titanium surface in both control and test substrates, with unchanged surface roughness with respect to the untreated surfaces. All samples show the deposition of extracellular matrix, more pronounced in the test and GO-functionalized test discs. GO-functionalized test samples evidenced a significant viability, with no cytotoxic response and a remarkable early stage proliferation of DPSCs cells, followed by their successful differentiation into osteoblasts. Conclusions: The described protocol of GO-functionalization provides a novel not cytotoxic biomaterial that is able to stimulate cell viability and that better and more quickly induces osteogenic differentiation with respect to simple titanium discs. Our findings pave the way to exploit this GO-functionalization protocol for the production of novel dental implant materials that display improved integration with the host tissue. MDPI 2020-04-01 /pmc/articles/PMC7221922/ /pubmed/32244572 http://dx.doi.org/10.3390/nano10040654 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Di Carlo, Roberta Di Crescenzo, Antonello Pilato, Serena Ventrella, Alessia Piattelli, Adriano Recinella, Lucia Chiavaroli, Annalisa Giordani, Silvia Baldrighi, Michele Camisasca, Adalberto Zavan, Barbara Falconi, Mirella Cataldi, Amelia Fontana, Antonella Zara, Susi Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study |
title | Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study |
title_full | Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study |
title_fullStr | Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study |
title_full_unstemmed | Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study |
title_short | Osteoblastic Differentiation on Graphene Oxide-Functionalized Titanium Surfaces: An In Vitro Study |
title_sort | osteoblastic differentiation on graphene oxide-functionalized titanium surfaces: an in vitro study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221922/ https://www.ncbi.nlm.nih.gov/pubmed/32244572 http://dx.doi.org/10.3390/nano10040654 |
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