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Impact of Isoorientin on Metabolic Activity and Lipid Accumulation in Differentiated Adipocytes

The current study explored the effect of isoorientin on the metabolic activity and lipid accumulation in fully differentiated 3T3-L1 adipocytes. To achieve this, the 3T3-L1 pre-adipocytes were differentiated for eight days and treated with various concentrations of isoorientin (0.1–100 μM) for four...

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Autores principales: Ziqubu, Khanyisani, Muller, Christo J. F., Dludla, Phiwayinkosi V., Mthembu, Sinenhlanhla X. H., Obonye, Nnini, Louw, Johan, Kappo, Abidemi P., Silvestri, Sonia, Orlando, Patrick, Tiano, Luca, Mazibuko-Mbeje, Sithandiwe E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221924/
https://www.ncbi.nlm.nih.gov/pubmed/32294890
http://dx.doi.org/10.3390/molecules25081773
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author Ziqubu, Khanyisani
Muller, Christo J. F.
Dludla, Phiwayinkosi V.
Mthembu, Sinenhlanhla X. H.
Obonye, Nnini
Louw, Johan
Kappo, Abidemi P.
Silvestri, Sonia
Orlando, Patrick
Tiano, Luca
Mazibuko-Mbeje, Sithandiwe E.
author_facet Ziqubu, Khanyisani
Muller, Christo J. F.
Dludla, Phiwayinkosi V.
Mthembu, Sinenhlanhla X. H.
Obonye, Nnini
Louw, Johan
Kappo, Abidemi P.
Silvestri, Sonia
Orlando, Patrick
Tiano, Luca
Mazibuko-Mbeje, Sithandiwe E.
author_sort Ziqubu, Khanyisani
collection PubMed
description The current study explored the effect of isoorientin on the metabolic activity and lipid accumulation in fully differentiated 3T3-L1 adipocytes. To achieve this, the 3T3-L1 pre-adipocytes were differentiated for eight days and treated with various concentrations of isoorientin (0.1–100 μM) for four hours. Subsequently, the metabolic activity, lipid accumulation, and mitochondrial respiration were assessed. Furthermore, to unravel the molecular mechanisms that might elucidate the bioactivity of isoorientin, protein expression of the genes involved in insulin signaling and energy expenditure, such as AKT and AMPK, were investigated. The results showed that isoorientin, at different doses, could block lipid storage and enhance glycerol release, with a concomitant improvement of the metabolic activity and mitochondrial function. Although the observed beneficial effects of isoorientin on these cultured 3T3-L1 adipocytes were not consistent at all concentrations, it was clear that doses between 1 and 10 μM were most effective compared to the untreated control. Moreover, the activity of isoorientin was comparable to tested positive controls of CL-316,2431, isoproterenol, insulin, and metformin. Mechanistically, protein expression of AKT and AMPK, was enhanced with isoorientin exposure, suggesting their partial role in modulating lipid metabolism and mitochondrial biogenesis. Indeed, our results showed that isoorientin has the ability to enhance mitochondrial respiration, as we observed an increase in the ATP and oxygen consumption rate. Therefore, we concluded that isoorientin has a potential to impact mitochondrial activity, lipid metabolism and energy expenditure using an in vitro experimental model of obesity.
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spelling pubmed-72219242020-05-22 Impact of Isoorientin on Metabolic Activity and Lipid Accumulation in Differentiated Adipocytes Ziqubu, Khanyisani Muller, Christo J. F. Dludla, Phiwayinkosi V. Mthembu, Sinenhlanhla X. H. Obonye, Nnini Louw, Johan Kappo, Abidemi P. Silvestri, Sonia Orlando, Patrick Tiano, Luca Mazibuko-Mbeje, Sithandiwe E. Molecules Communication The current study explored the effect of isoorientin on the metabolic activity and lipid accumulation in fully differentiated 3T3-L1 adipocytes. To achieve this, the 3T3-L1 pre-adipocytes were differentiated for eight days and treated with various concentrations of isoorientin (0.1–100 μM) for four hours. Subsequently, the metabolic activity, lipid accumulation, and mitochondrial respiration were assessed. Furthermore, to unravel the molecular mechanisms that might elucidate the bioactivity of isoorientin, protein expression of the genes involved in insulin signaling and energy expenditure, such as AKT and AMPK, were investigated. The results showed that isoorientin, at different doses, could block lipid storage and enhance glycerol release, with a concomitant improvement of the metabolic activity and mitochondrial function. Although the observed beneficial effects of isoorientin on these cultured 3T3-L1 adipocytes were not consistent at all concentrations, it was clear that doses between 1 and 10 μM were most effective compared to the untreated control. Moreover, the activity of isoorientin was comparable to tested positive controls of CL-316,2431, isoproterenol, insulin, and metformin. Mechanistically, protein expression of AKT and AMPK, was enhanced with isoorientin exposure, suggesting their partial role in modulating lipid metabolism and mitochondrial biogenesis. Indeed, our results showed that isoorientin has the ability to enhance mitochondrial respiration, as we observed an increase in the ATP and oxygen consumption rate. Therefore, we concluded that isoorientin has a potential to impact mitochondrial activity, lipid metabolism and energy expenditure using an in vitro experimental model of obesity. MDPI 2020-04-13 /pmc/articles/PMC7221924/ /pubmed/32294890 http://dx.doi.org/10.3390/molecules25081773 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Ziqubu, Khanyisani
Muller, Christo J. F.
Dludla, Phiwayinkosi V.
Mthembu, Sinenhlanhla X. H.
Obonye, Nnini
Louw, Johan
Kappo, Abidemi P.
Silvestri, Sonia
Orlando, Patrick
Tiano, Luca
Mazibuko-Mbeje, Sithandiwe E.
Impact of Isoorientin on Metabolic Activity and Lipid Accumulation in Differentiated Adipocytes
title Impact of Isoorientin on Metabolic Activity and Lipid Accumulation in Differentiated Adipocytes
title_full Impact of Isoorientin on Metabolic Activity and Lipid Accumulation in Differentiated Adipocytes
title_fullStr Impact of Isoorientin on Metabolic Activity and Lipid Accumulation in Differentiated Adipocytes
title_full_unstemmed Impact of Isoorientin on Metabolic Activity and Lipid Accumulation in Differentiated Adipocytes
title_short Impact of Isoorientin on Metabolic Activity and Lipid Accumulation in Differentiated Adipocytes
title_sort impact of isoorientin on metabolic activity and lipid accumulation in differentiated adipocytes
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221924/
https://www.ncbi.nlm.nih.gov/pubmed/32294890
http://dx.doi.org/10.3390/molecules25081773
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