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The Oxidation-Induced Autofluorescence Hypothesis: Red Edge Excitation and Implications for Metabolic Imaging
Endogenous autofluorescence of biological tissues is an important source of information for biomedical diagnostics. Despite the molecular complexity of biological tissues, the list of commonly known fluorophores is strictly limited. Still, the question of molecular sources of the red and near-infrar...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221974/ https://www.ncbi.nlm.nih.gov/pubmed/32316642 http://dx.doi.org/10.3390/molecules25081863 |
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author | Semenov, Alexey N. Yakimov, Boris P. Rubekina, Anna A. Gorin, Dmitry A. Drachev, Vladimir P. Zarubin, Mikhail P. Velikanov, Alexander N. Lademann, Juergen Fadeev, Victor V. Priezzhev, Alexander V. Darvin, Maxim E. Shirshin, Evgeny A. |
author_facet | Semenov, Alexey N. Yakimov, Boris P. Rubekina, Anna A. Gorin, Dmitry A. Drachev, Vladimir P. Zarubin, Mikhail P. Velikanov, Alexander N. Lademann, Juergen Fadeev, Victor V. Priezzhev, Alexander V. Darvin, Maxim E. Shirshin, Evgeny A. |
author_sort | Semenov, Alexey N. |
collection | PubMed |
description | Endogenous autofluorescence of biological tissues is an important source of information for biomedical diagnostics. Despite the molecular complexity of biological tissues, the list of commonly known fluorophores is strictly limited. Still, the question of molecular sources of the red and near-infrared excited autofluorescence remains open. In this work we demonstrated that the oxidation products of organic components (lipids, proteins, amino acids, etc.) can serve as the molecular source of such red and near-infrared excited autofluorescence. Using model solutions and cell systems (human keratinocytes) under oxidative stress induced by UV irradiation we demonstrated that oxidation products can contribute significantly to the autofluorescence signal of biological systems in the entire visible range of the spectrum, even at the emission and excitation wavelengths higher than 650 nm. The obtained results suggest the principal possibility to explain the red fluorescence excitation in a large class of biosystems—aggregates of proteins and peptides, cells and tissues—by the impact of oxidation products, since oxidation products are inevitably presented in the tissue. The observed fluorescence signal with broad excitation originated from oxidation products may also lead to the alteration of metabolic imaging results and has to be taken into account. |
format | Online Article Text |
id | pubmed-7221974 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72219742020-05-22 The Oxidation-Induced Autofluorescence Hypothesis: Red Edge Excitation and Implications for Metabolic Imaging Semenov, Alexey N. Yakimov, Boris P. Rubekina, Anna A. Gorin, Dmitry A. Drachev, Vladimir P. Zarubin, Mikhail P. Velikanov, Alexander N. Lademann, Juergen Fadeev, Victor V. Priezzhev, Alexander V. Darvin, Maxim E. Shirshin, Evgeny A. Molecules Article Endogenous autofluorescence of biological tissues is an important source of information for biomedical diagnostics. Despite the molecular complexity of biological tissues, the list of commonly known fluorophores is strictly limited. Still, the question of molecular sources of the red and near-infrared excited autofluorescence remains open. In this work we demonstrated that the oxidation products of organic components (lipids, proteins, amino acids, etc.) can serve as the molecular source of such red and near-infrared excited autofluorescence. Using model solutions and cell systems (human keratinocytes) under oxidative stress induced by UV irradiation we demonstrated that oxidation products can contribute significantly to the autofluorescence signal of biological systems in the entire visible range of the spectrum, even at the emission and excitation wavelengths higher than 650 nm. The obtained results suggest the principal possibility to explain the red fluorescence excitation in a large class of biosystems—aggregates of proteins and peptides, cells and tissues—by the impact of oxidation products, since oxidation products are inevitably presented in the tissue. The observed fluorescence signal with broad excitation originated from oxidation products may also lead to the alteration of metabolic imaging results and has to be taken into account. MDPI 2020-04-17 /pmc/articles/PMC7221974/ /pubmed/32316642 http://dx.doi.org/10.3390/molecules25081863 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Semenov, Alexey N. Yakimov, Boris P. Rubekina, Anna A. Gorin, Dmitry A. Drachev, Vladimir P. Zarubin, Mikhail P. Velikanov, Alexander N. Lademann, Juergen Fadeev, Victor V. Priezzhev, Alexander V. Darvin, Maxim E. Shirshin, Evgeny A. The Oxidation-Induced Autofluorescence Hypothesis: Red Edge Excitation and Implications for Metabolic Imaging |
title | The Oxidation-Induced Autofluorescence Hypothesis: Red Edge Excitation and Implications for Metabolic Imaging |
title_full | The Oxidation-Induced Autofluorescence Hypothesis: Red Edge Excitation and Implications for Metabolic Imaging |
title_fullStr | The Oxidation-Induced Autofluorescence Hypothesis: Red Edge Excitation and Implications for Metabolic Imaging |
title_full_unstemmed | The Oxidation-Induced Autofluorescence Hypothesis: Red Edge Excitation and Implications for Metabolic Imaging |
title_short | The Oxidation-Induced Autofluorescence Hypothesis: Red Edge Excitation and Implications for Metabolic Imaging |
title_sort | oxidation-induced autofluorescence hypothesis: red edge excitation and implications for metabolic imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221974/ https://www.ncbi.nlm.nih.gov/pubmed/32316642 http://dx.doi.org/10.3390/molecules25081863 |
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