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Dry Generation of CeO(2) Nanoparticles and Deposition onto a Co-Culture of A549 and THP-1 Cells in Air-Liquid Interface—Dosimetry Considerations and Comparison to Submerged Exposure
Relevant in vitro assays that can simulate exposure to nanoparticles (NPs) via inhalation are urgently needed. Presently, the most common method employed is to expose lung cells under submerged conditions, but the cellular responses to NPs under such conditions might differ from those observed at th...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221976/ https://www.ncbi.nlm.nih.gov/pubmed/32230801 http://dx.doi.org/10.3390/nano10040618 |
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author | Cappellini, Francesca Di Bucchianico, Sebastiano Karri, Venkatanaidu Latvala, Siiri Malmlöf, Maria Kippler, Maria Elihn, Karine Hedberg, Jonas Odnevall Wallinder, Inger Gerde, Per Karlsson, Hanna L. |
author_facet | Cappellini, Francesca Di Bucchianico, Sebastiano Karri, Venkatanaidu Latvala, Siiri Malmlöf, Maria Kippler, Maria Elihn, Karine Hedberg, Jonas Odnevall Wallinder, Inger Gerde, Per Karlsson, Hanna L. |
author_sort | Cappellini, Francesca |
collection | PubMed |
description | Relevant in vitro assays that can simulate exposure to nanoparticles (NPs) via inhalation are urgently needed. Presently, the most common method employed is to expose lung cells under submerged conditions, but the cellular responses to NPs under such conditions might differ from those observed at the more physiological air-liquid interface (ALI). The aim of this study was to investigate the cytotoxic and inflammatory potential of CeO(2) NPs (NM-212) in a co-culture of A549 lung epithelial cells and differentiated THP-1 cells in both ALI and submerged conditions. Cellular dose was examined quantitatively using inductively coupled plasma mass spectrometry (ICP-MS). The role of serum and LPS-priming for IL-1β release was further tested in THP-1 cells in submerged exposure. An aerosol of CeO(2) NPs was generated by using the PreciseInhale(®) system, and NPs were deposited on the co-culture using XposeALI(®). No or minor cytotoxicity and no increased release of inflammatory cytokines (IL-1β, IL-6, TNFα, MCP-1) were observed after exposure of the co-culture in ALI (max 5 µg/cm(2)) or submerged (max 22 µg/cm(2)) conditions. In contrast, CeO(2) NPs cause clear IL-1β release in monocultures of macrophage-like THP-1, independent of the presence of serum and LPS-priming. This study demonstrates a useful approach for comparing effects at various in-vitro conditions. |
format | Online Article Text |
id | pubmed-7221976 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72219762020-05-22 Dry Generation of CeO(2) Nanoparticles and Deposition onto a Co-Culture of A549 and THP-1 Cells in Air-Liquid Interface—Dosimetry Considerations and Comparison to Submerged Exposure Cappellini, Francesca Di Bucchianico, Sebastiano Karri, Venkatanaidu Latvala, Siiri Malmlöf, Maria Kippler, Maria Elihn, Karine Hedberg, Jonas Odnevall Wallinder, Inger Gerde, Per Karlsson, Hanna L. Nanomaterials (Basel) Article Relevant in vitro assays that can simulate exposure to nanoparticles (NPs) via inhalation are urgently needed. Presently, the most common method employed is to expose lung cells under submerged conditions, but the cellular responses to NPs under such conditions might differ from those observed at the more physiological air-liquid interface (ALI). The aim of this study was to investigate the cytotoxic and inflammatory potential of CeO(2) NPs (NM-212) in a co-culture of A549 lung epithelial cells and differentiated THP-1 cells in both ALI and submerged conditions. Cellular dose was examined quantitatively using inductively coupled plasma mass spectrometry (ICP-MS). The role of serum and LPS-priming for IL-1β release was further tested in THP-1 cells in submerged exposure. An aerosol of CeO(2) NPs was generated by using the PreciseInhale(®) system, and NPs were deposited on the co-culture using XposeALI(®). No or minor cytotoxicity and no increased release of inflammatory cytokines (IL-1β, IL-6, TNFα, MCP-1) were observed after exposure of the co-culture in ALI (max 5 µg/cm(2)) or submerged (max 22 µg/cm(2)) conditions. In contrast, CeO(2) NPs cause clear IL-1β release in monocultures of macrophage-like THP-1, independent of the presence of serum and LPS-priming. This study demonstrates a useful approach for comparing effects at various in-vitro conditions. MDPI 2020-03-27 /pmc/articles/PMC7221976/ /pubmed/32230801 http://dx.doi.org/10.3390/nano10040618 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cappellini, Francesca Di Bucchianico, Sebastiano Karri, Venkatanaidu Latvala, Siiri Malmlöf, Maria Kippler, Maria Elihn, Karine Hedberg, Jonas Odnevall Wallinder, Inger Gerde, Per Karlsson, Hanna L. Dry Generation of CeO(2) Nanoparticles and Deposition onto a Co-Culture of A549 and THP-1 Cells in Air-Liquid Interface—Dosimetry Considerations and Comparison to Submerged Exposure |
title | Dry Generation of CeO(2) Nanoparticles and Deposition onto a Co-Culture of A549 and THP-1 Cells in Air-Liquid Interface—Dosimetry Considerations and Comparison to Submerged Exposure |
title_full | Dry Generation of CeO(2) Nanoparticles and Deposition onto a Co-Culture of A549 and THP-1 Cells in Air-Liquid Interface—Dosimetry Considerations and Comparison to Submerged Exposure |
title_fullStr | Dry Generation of CeO(2) Nanoparticles and Deposition onto a Co-Culture of A549 and THP-1 Cells in Air-Liquid Interface—Dosimetry Considerations and Comparison to Submerged Exposure |
title_full_unstemmed | Dry Generation of CeO(2) Nanoparticles and Deposition onto a Co-Culture of A549 and THP-1 Cells in Air-Liquid Interface—Dosimetry Considerations and Comparison to Submerged Exposure |
title_short | Dry Generation of CeO(2) Nanoparticles and Deposition onto a Co-Culture of A549 and THP-1 Cells in Air-Liquid Interface—Dosimetry Considerations and Comparison to Submerged Exposure |
title_sort | dry generation of ceo(2) nanoparticles and deposition onto a co-culture of a549 and thp-1 cells in air-liquid interface—dosimetry considerations and comparison to submerged exposure |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221976/ https://www.ncbi.nlm.nih.gov/pubmed/32230801 http://dx.doi.org/10.3390/nano10040618 |
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