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Potential Synergy between Spores of Metarhizium anisopliae and Plant Secondary Metabolite, 1-Chlorooctadecane for Effective Natural Acaricide Development

Date palm dust mites are important pests severely infesting valuable nutritious fruits (dates) of date palm. In search of an alternative to acaricides, joint action of Metarhizium anisopliae EBCL 02049 spores and 1-Chlorooctadecane was evaluated as a potential candidate for the management of Oligony...

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Autores principales: Hussain, Abid, AlJabr, Ahmed Mohammed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221990/
https://www.ncbi.nlm.nih.gov/pubmed/32326009
http://dx.doi.org/10.3390/molecules25081900
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author Hussain, Abid
AlJabr, Ahmed Mohammed
author_facet Hussain, Abid
AlJabr, Ahmed Mohammed
author_sort Hussain, Abid
collection PubMed
description Date palm dust mites are important pests severely infesting valuable nutritious fruits (dates) of date palm. In search of an alternative to acaricides, joint action of Metarhizium anisopliae EBCL 02049 spores and 1-Chlorooctadecane was evaluated as a potential candidate for the management of Oligonychus afrasiaticus through natural products. In this regard, in vitro tests were performed to evaluate the interaction of M. anisopliae spores with multiple doses of 1-Chlorooctadecane (0.8, 1.6, 2.4, 3.2, and 4.0 mg/mL). Compatibility bioassay results evidenced from vegetative growth (77.7–84.40 mm), sporulation (5.50–7.30 × 10(6) spores/mL), and germination (96.70–98.20%), revealed that all the tested doses are compatible (biological index > 82) with the spores of M. anisopliae. The impact of combined treatment of spores with 1-Chlorooctadecane in different proportions (Scheme I, II, III, and IV) compared to their sole application against O. afrasiaticus was evaluated by concentration–mortality response bioassays. Results showed that all the combined treatments revealed high mortality compared to the sole application, which showed relatively slow mortality response over time. Toxicity recorded from Scheme IV combinations (80% 1-Chlorooctadecane: 20% Spores), exhibited strong synergistic interaction (joint toxicity = 713). Furthermore, potent interactions have overcome the host antioxidant defense at the final stage of infection by tremendously reducing catalase, and superoxide dismutase activities. These experiments demonstrated fungal–toxin joint synergistic interaction as a promising date palm dust mite management option.
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spelling pubmed-72219902020-05-22 Potential Synergy between Spores of Metarhizium anisopliae and Plant Secondary Metabolite, 1-Chlorooctadecane for Effective Natural Acaricide Development Hussain, Abid AlJabr, Ahmed Mohammed Molecules Article Date palm dust mites are important pests severely infesting valuable nutritious fruits (dates) of date palm. In search of an alternative to acaricides, joint action of Metarhizium anisopliae EBCL 02049 spores and 1-Chlorooctadecane was evaluated as a potential candidate for the management of Oligonychus afrasiaticus through natural products. In this regard, in vitro tests were performed to evaluate the interaction of M. anisopliae spores with multiple doses of 1-Chlorooctadecane (0.8, 1.6, 2.4, 3.2, and 4.0 mg/mL). Compatibility bioassay results evidenced from vegetative growth (77.7–84.40 mm), sporulation (5.50–7.30 × 10(6) spores/mL), and germination (96.70–98.20%), revealed that all the tested doses are compatible (biological index > 82) with the spores of M. anisopliae. The impact of combined treatment of spores with 1-Chlorooctadecane in different proportions (Scheme I, II, III, and IV) compared to their sole application against O. afrasiaticus was evaluated by concentration–mortality response bioassays. Results showed that all the combined treatments revealed high mortality compared to the sole application, which showed relatively slow mortality response over time. Toxicity recorded from Scheme IV combinations (80% 1-Chlorooctadecane: 20% Spores), exhibited strong synergistic interaction (joint toxicity = 713). Furthermore, potent interactions have overcome the host antioxidant defense at the final stage of infection by tremendously reducing catalase, and superoxide dismutase activities. These experiments demonstrated fungal–toxin joint synergistic interaction as a promising date palm dust mite management option. MDPI 2020-04-20 /pmc/articles/PMC7221990/ /pubmed/32326009 http://dx.doi.org/10.3390/molecules25081900 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hussain, Abid
AlJabr, Ahmed Mohammed
Potential Synergy between Spores of Metarhizium anisopliae and Plant Secondary Metabolite, 1-Chlorooctadecane for Effective Natural Acaricide Development
title Potential Synergy between Spores of Metarhizium anisopliae and Plant Secondary Metabolite, 1-Chlorooctadecane for Effective Natural Acaricide Development
title_full Potential Synergy between Spores of Metarhizium anisopliae and Plant Secondary Metabolite, 1-Chlorooctadecane for Effective Natural Acaricide Development
title_fullStr Potential Synergy between Spores of Metarhizium anisopliae and Plant Secondary Metabolite, 1-Chlorooctadecane for Effective Natural Acaricide Development
title_full_unstemmed Potential Synergy between Spores of Metarhizium anisopliae and Plant Secondary Metabolite, 1-Chlorooctadecane for Effective Natural Acaricide Development
title_short Potential Synergy between Spores of Metarhizium anisopliae and Plant Secondary Metabolite, 1-Chlorooctadecane for Effective Natural Acaricide Development
title_sort potential synergy between spores of metarhizium anisopliae and plant secondary metabolite, 1-chlorooctadecane for effective natural acaricide development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7221990/
https://www.ncbi.nlm.nih.gov/pubmed/32326009
http://dx.doi.org/10.3390/molecules25081900
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