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Impact of Ultrasound Extraction Parameters on the Antioxidant Properties of Moringa Oleifera Leaves

Recently, much interest has been focused on Moringa oleifera L., a highly versatile and sustainable plant. In addition to its nutritional properties, numerous bioactive compounds have been identified in M. oleifera leaves, for which healthy properties have been reported. In the present research, the...

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Autores principales: Pollini, Luna, Tringaniello, Carmela, Ianni, Federica, Blasi, Francesca, Manes, Jordi, Cossignani, Lina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222185/
https://www.ncbi.nlm.nih.gov/pubmed/32224892
http://dx.doi.org/10.3390/antiox9040277
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author Pollini, Luna
Tringaniello, Carmela
Ianni, Federica
Blasi, Francesca
Manes, Jordi
Cossignani, Lina
author_facet Pollini, Luna
Tringaniello, Carmela
Ianni, Federica
Blasi, Francesca
Manes, Jordi
Cossignani, Lina
author_sort Pollini, Luna
collection PubMed
description Recently, much interest has been focused on Moringa oleifera L., a highly versatile and sustainable plant. In addition to its nutritional properties, numerous bioactive compounds have been identified in M. oleifera leaves, for which healthy properties have been reported. In the present research, the impact of ultrasound-assisted extraction (UAE) on the recovery of the bioactive compounds from leaves was investigated. Firstly, an experimental design approach has been used to highlight the influence of some extraction parameters (solvent, solvent/dry leaves ratio, temperature, time) on phenol compound recovery and antioxidant activity. Solvent composition was the most influential factor; in fact, the presence of water in the solvent (50:50, v/v) corresponded to an increase in the extraction performance. The liquid/solid ratio (L/S) also influenced the extraction process; in fact, the total phenol content reached 13.4 mg gallic acid equivalent (GAE)/g dry matter (DM) in the following UAE conditions: 50% water, 60:1 L/S ratio, 60 °C, 60 min. In order to quantify flavonols, hydroalcoholic extracts were analysed by HPLC-DAD (high performance liquid chromatography-diode array detector). In the flavonol class, the glycosidic forms of quercetin and kaempferol were mainly detected. Their content ranged from 216.4 µg/g DM of quercetin 3-O-rhamnoside to 293.9 µg/g DM of quercetin 3-O-(6″-O-malonyl)-β-D-glucoside. In summary, the leaves of M. oleifera are a potential natural source of bioactive compounds, proving to be very promising for the development of health-promoting food supplements.
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spelling pubmed-72221852020-05-28 Impact of Ultrasound Extraction Parameters on the Antioxidant Properties of Moringa Oleifera Leaves Pollini, Luna Tringaniello, Carmela Ianni, Federica Blasi, Francesca Manes, Jordi Cossignani, Lina Antioxidants (Basel) Article Recently, much interest has been focused on Moringa oleifera L., a highly versatile and sustainable plant. In addition to its nutritional properties, numerous bioactive compounds have been identified in M. oleifera leaves, for which healthy properties have been reported. In the present research, the impact of ultrasound-assisted extraction (UAE) on the recovery of the bioactive compounds from leaves was investigated. Firstly, an experimental design approach has been used to highlight the influence of some extraction parameters (solvent, solvent/dry leaves ratio, temperature, time) on phenol compound recovery and antioxidant activity. Solvent composition was the most influential factor; in fact, the presence of water in the solvent (50:50, v/v) corresponded to an increase in the extraction performance. The liquid/solid ratio (L/S) also influenced the extraction process; in fact, the total phenol content reached 13.4 mg gallic acid equivalent (GAE)/g dry matter (DM) in the following UAE conditions: 50% water, 60:1 L/S ratio, 60 °C, 60 min. In order to quantify flavonols, hydroalcoholic extracts were analysed by HPLC-DAD (high performance liquid chromatography-diode array detector). In the flavonol class, the glycosidic forms of quercetin and kaempferol were mainly detected. Their content ranged from 216.4 µg/g DM of quercetin 3-O-rhamnoside to 293.9 µg/g DM of quercetin 3-O-(6″-O-malonyl)-β-D-glucoside. In summary, the leaves of M. oleifera are a potential natural source of bioactive compounds, proving to be very promising for the development of health-promoting food supplements. MDPI 2020-03-26 /pmc/articles/PMC7222185/ /pubmed/32224892 http://dx.doi.org/10.3390/antiox9040277 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pollini, Luna
Tringaniello, Carmela
Ianni, Federica
Blasi, Francesca
Manes, Jordi
Cossignani, Lina
Impact of Ultrasound Extraction Parameters on the Antioxidant Properties of Moringa Oleifera Leaves
title Impact of Ultrasound Extraction Parameters on the Antioxidant Properties of Moringa Oleifera Leaves
title_full Impact of Ultrasound Extraction Parameters on the Antioxidant Properties of Moringa Oleifera Leaves
title_fullStr Impact of Ultrasound Extraction Parameters on the Antioxidant Properties of Moringa Oleifera Leaves
title_full_unstemmed Impact of Ultrasound Extraction Parameters on the Antioxidant Properties of Moringa Oleifera Leaves
title_short Impact of Ultrasound Extraction Parameters on the Antioxidant Properties of Moringa Oleifera Leaves
title_sort impact of ultrasound extraction parameters on the antioxidant properties of moringa oleifera leaves
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222185/
https://www.ncbi.nlm.nih.gov/pubmed/32224892
http://dx.doi.org/10.3390/antiox9040277
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