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Targeted imaging of orthotopic prostate cancer by using clinical transformable photoacoustic molecular probe
BACKGROUND: To obtain high-yield histological samples by targeted prostate cancer (PCa) biopsy is the current trend compared with transrectal ultrasound (TRUS)-guided systematic histological biopsy, which is regarded as the gold standard for prostate cancer (PCa) diagnosis. In this paper, we present...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222516/ https://www.ncbi.nlm.nih.gov/pubmed/32410590 http://dx.doi.org/10.1186/s12885-020-06801-9 |
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author | Qiu, Chen Bai, Yuanyuan Yin, Tinghui Miao, Xiaoyan Gao, Rongkang Zhou, Huichao Ren, Jie Song, Liang Liu, Chengbo Zheng, Hairong Zheng, Rongqin |
author_facet | Qiu, Chen Bai, Yuanyuan Yin, Tinghui Miao, Xiaoyan Gao, Rongkang Zhou, Huichao Ren, Jie Song, Liang Liu, Chengbo Zheng, Hairong Zheng, Rongqin |
author_sort | Qiu, Chen |
collection | PubMed |
description | BACKGROUND: To obtain high-yield histological samples by targeted prostate cancer (PCa) biopsy is the current trend compared with transrectal ultrasound (TRUS)-guided systematic histological biopsy, which is regarded as the gold standard for prostate cancer (PCa) diagnosis. In this paper, we present a targeted PCa imaging strategy using a real-time molecular photoacoustic imaging system integrated with a handheld US probe (PAI/US) and synthesized an integrin α(v)β(3) targeted probe based on ICG (cRGD–ICG). METHODS: To prepare cRGD–ICG, ICG-NHS was linked to cRGD through carboxyl-co-reaction. In vitro PA imaging ability of cRGD–ICG was tested. Orthotopic PCa-bearing rats were used as animal models. After injected with either cRGD–ICG or non-targeted probe, rats were implemented with PA imaging to confirm the specific accumulation of cRGD–ICG at tumor region. Moreover, pathological frozen slices were made to observe distribution of the probe in prostate tissue ex vivo. RESULTS: A small molecular PAI probe was synthesized and exhibited excellent targeted imaging ability in vitro. In vivo photoacoustic imaging was carried out after intravenous injection of cRGD-ICG in orthotopic PCa-bearing rats under the facilitation of the PAI/US system. Maximum molecular photoacoustic signals were observed in the tumor area in vivo after the probe injection, which showed 3.8-fold higher signal enhancement than that in the control group (P < 0.05). Significantly higher cRGD-ICG accumulation was observed under confocal microscopy in the tumor region than in normal prostate tissue. CONCLUSIONS: All our results showed that the comprehensive strategy provided a high-yield and reliable method for PCa diagnosis and targeted prostate biopsy, with great clinical translation potential. |
format | Online Article Text |
id | pubmed-7222516 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-72225162020-05-20 Targeted imaging of orthotopic prostate cancer by using clinical transformable photoacoustic molecular probe Qiu, Chen Bai, Yuanyuan Yin, Tinghui Miao, Xiaoyan Gao, Rongkang Zhou, Huichao Ren, Jie Song, Liang Liu, Chengbo Zheng, Hairong Zheng, Rongqin BMC Cancer Research Article BACKGROUND: To obtain high-yield histological samples by targeted prostate cancer (PCa) biopsy is the current trend compared with transrectal ultrasound (TRUS)-guided systematic histological biopsy, which is regarded as the gold standard for prostate cancer (PCa) diagnosis. In this paper, we present a targeted PCa imaging strategy using a real-time molecular photoacoustic imaging system integrated with a handheld US probe (PAI/US) and synthesized an integrin α(v)β(3) targeted probe based on ICG (cRGD–ICG). METHODS: To prepare cRGD–ICG, ICG-NHS was linked to cRGD through carboxyl-co-reaction. In vitro PA imaging ability of cRGD–ICG was tested. Orthotopic PCa-bearing rats were used as animal models. After injected with either cRGD–ICG or non-targeted probe, rats were implemented with PA imaging to confirm the specific accumulation of cRGD–ICG at tumor region. Moreover, pathological frozen slices were made to observe distribution of the probe in prostate tissue ex vivo. RESULTS: A small molecular PAI probe was synthesized and exhibited excellent targeted imaging ability in vitro. In vivo photoacoustic imaging was carried out after intravenous injection of cRGD-ICG in orthotopic PCa-bearing rats under the facilitation of the PAI/US system. Maximum molecular photoacoustic signals were observed in the tumor area in vivo after the probe injection, which showed 3.8-fold higher signal enhancement than that in the control group (P < 0.05). Significantly higher cRGD-ICG accumulation was observed under confocal microscopy in the tumor region than in normal prostate tissue. CONCLUSIONS: All our results showed that the comprehensive strategy provided a high-yield and reliable method for PCa diagnosis and targeted prostate biopsy, with great clinical translation potential. BioMed Central 2020-05-14 /pmc/articles/PMC7222516/ /pubmed/32410590 http://dx.doi.org/10.1186/s12885-020-06801-9 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Qiu, Chen Bai, Yuanyuan Yin, Tinghui Miao, Xiaoyan Gao, Rongkang Zhou, Huichao Ren, Jie Song, Liang Liu, Chengbo Zheng, Hairong Zheng, Rongqin Targeted imaging of orthotopic prostate cancer by using clinical transformable photoacoustic molecular probe |
title | Targeted imaging of orthotopic prostate cancer by using clinical transformable photoacoustic molecular probe |
title_full | Targeted imaging of orthotopic prostate cancer by using clinical transformable photoacoustic molecular probe |
title_fullStr | Targeted imaging of orthotopic prostate cancer by using clinical transformable photoacoustic molecular probe |
title_full_unstemmed | Targeted imaging of orthotopic prostate cancer by using clinical transformable photoacoustic molecular probe |
title_short | Targeted imaging of orthotopic prostate cancer by using clinical transformable photoacoustic molecular probe |
title_sort | targeted imaging of orthotopic prostate cancer by using clinical transformable photoacoustic molecular probe |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222516/ https://www.ncbi.nlm.nih.gov/pubmed/32410590 http://dx.doi.org/10.1186/s12885-020-06801-9 |
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