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Effects of Tributyrin Supplementation on Growth Performance, Insulin, Blood Metabolites and Gut Microbiota in Weaned Piglets

SIMPLE SUMMARY: In animal farming, alternatives to antibiotics are required due to the increase of antimicrobial resistance. In this contest, tributyrin showed the ability to promote gut health, to modulate gut microbiota and to improve protein digestibility, leading also to higher growth performanc...

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Autores principales: Sotira, Stefania, Dell’Anno, Matteo, Caprarulo, Valentina, Hejna, Monika, Pirrone, Federica, Callegari, Maria Luisa, Tucci, Telma Vieira, Rossi, Luciana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222802/
https://www.ncbi.nlm.nih.gov/pubmed/32331306
http://dx.doi.org/10.3390/ani10040726
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author Sotira, Stefania
Dell’Anno, Matteo
Caprarulo, Valentina
Hejna, Monika
Pirrone, Federica
Callegari, Maria Luisa
Tucci, Telma Vieira
Rossi, Luciana
author_facet Sotira, Stefania
Dell’Anno, Matteo
Caprarulo, Valentina
Hejna, Monika
Pirrone, Federica
Callegari, Maria Luisa
Tucci, Telma Vieira
Rossi, Luciana
author_sort Sotira, Stefania
collection PubMed
description SIMPLE SUMMARY: In animal farming, alternatives to antibiotics are required due to the increase of antimicrobial resistance. In this contest, tributyrin showed the ability to promote gut health, to modulate gut microbiota and to improve protein digestibility, leading also to higher growth performance. However, although the mode of action of tributyrin on the intestinal epithelial cells has been partially explained, its effects on lipid and protein metabolism needs to be investigated. This paper provides information about the influence of tributyrin on production traits, blood parameters, faecal microbiota and faecal protein excretion in weaned piglets. ABSTRACT: The aim of this study was to investigate the effects of tributyrin supplementation on the production traits, the main metabolic parameters and gut microbiota in weaned piglets. One hundred and twenty crossbred piglets (Large White × Landrace) were randomly divided into two experimental groups (six pens each; 10 piglets per pen): the control group (CTRL), that received a basal diet, and the tributyrin group (TRIB) that received the basal diet supplemented with 0.2% tributyrin. The experimental period lasted 40 days. Production traits were measured at days 14, 28 and 40. A subset composed of 48 animals (n = 4 for each pen; n = 24 per group) was considered for the evaluation of serum metabolic parameters and hair cortisol by enzyme-linked immunosorbent assay (ELISA), and faecal microbiota by real-time polymerase chain reaction (PCR). Our results showed that the treatment significantly increased body weight (BW) at day 28 and day 40 (p = 0.0279 and p = 0.0006, respectively) and average daily gain (ADG) from day 28 to day 40 (p = 0.046). Gain to feed ratio (G:F) was significantly higher throughout the experimental period (p = 0.049). Even if the serum parameters were in the physiological range, albumin, albumin/globulin (A/G) ratio, glucose and high-density lipoproteins (HDL) fraction were significantly higher in the TRIB group. On the contrary, tributyrin significantly decreased the urea blood concentration (p = 0.0026), which was correlated with lean gain and feed efficiency. Moreover, serum insulin concentration, which has a regulatory effect on protein and lipid metabolism, was significantly higher in the TRIB group (p = 0.0187). In conclusion, this study demonstrated that tributyrin can be considered as a valid feed additive for weaned piglets.
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spelling pubmed-72228022020-05-18 Effects of Tributyrin Supplementation on Growth Performance, Insulin, Blood Metabolites and Gut Microbiota in Weaned Piglets Sotira, Stefania Dell’Anno, Matteo Caprarulo, Valentina Hejna, Monika Pirrone, Federica Callegari, Maria Luisa Tucci, Telma Vieira Rossi, Luciana Animals (Basel) Article SIMPLE SUMMARY: In animal farming, alternatives to antibiotics are required due to the increase of antimicrobial resistance. In this contest, tributyrin showed the ability to promote gut health, to modulate gut microbiota and to improve protein digestibility, leading also to higher growth performance. However, although the mode of action of tributyrin on the intestinal epithelial cells has been partially explained, its effects on lipid and protein metabolism needs to be investigated. This paper provides information about the influence of tributyrin on production traits, blood parameters, faecal microbiota and faecal protein excretion in weaned piglets. ABSTRACT: The aim of this study was to investigate the effects of tributyrin supplementation on the production traits, the main metabolic parameters and gut microbiota in weaned piglets. One hundred and twenty crossbred piglets (Large White × Landrace) were randomly divided into two experimental groups (six pens each; 10 piglets per pen): the control group (CTRL), that received a basal diet, and the tributyrin group (TRIB) that received the basal diet supplemented with 0.2% tributyrin. The experimental period lasted 40 days. Production traits were measured at days 14, 28 and 40. A subset composed of 48 animals (n = 4 for each pen; n = 24 per group) was considered for the evaluation of serum metabolic parameters and hair cortisol by enzyme-linked immunosorbent assay (ELISA), and faecal microbiota by real-time polymerase chain reaction (PCR). Our results showed that the treatment significantly increased body weight (BW) at day 28 and day 40 (p = 0.0279 and p = 0.0006, respectively) and average daily gain (ADG) from day 28 to day 40 (p = 0.046). Gain to feed ratio (G:F) was significantly higher throughout the experimental period (p = 0.049). Even if the serum parameters were in the physiological range, albumin, albumin/globulin (A/G) ratio, glucose and high-density lipoproteins (HDL) fraction were significantly higher in the TRIB group. On the contrary, tributyrin significantly decreased the urea blood concentration (p = 0.0026), which was correlated with lean gain and feed efficiency. Moreover, serum insulin concentration, which has a regulatory effect on protein and lipid metabolism, was significantly higher in the TRIB group (p = 0.0187). In conclusion, this study demonstrated that tributyrin can be considered as a valid feed additive for weaned piglets. MDPI 2020-04-22 /pmc/articles/PMC7222802/ /pubmed/32331306 http://dx.doi.org/10.3390/ani10040726 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sotira, Stefania
Dell’Anno, Matteo
Caprarulo, Valentina
Hejna, Monika
Pirrone, Federica
Callegari, Maria Luisa
Tucci, Telma Vieira
Rossi, Luciana
Effects of Tributyrin Supplementation on Growth Performance, Insulin, Blood Metabolites and Gut Microbiota in Weaned Piglets
title Effects of Tributyrin Supplementation on Growth Performance, Insulin, Blood Metabolites and Gut Microbiota in Weaned Piglets
title_full Effects of Tributyrin Supplementation on Growth Performance, Insulin, Blood Metabolites and Gut Microbiota in Weaned Piglets
title_fullStr Effects of Tributyrin Supplementation on Growth Performance, Insulin, Blood Metabolites and Gut Microbiota in Weaned Piglets
title_full_unstemmed Effects of Tributyrin Supplementation on Growth Performance, Insulin, Blood Metabolites and Gut Microbiota in Weaned Piglets
title_short Effects of Tributyrin Supplementation on Growth Performance, Insulin, Blood Metabolites and Gut Microbiota in Weaned Piglets
title_sort effects of tributyrin supplementation on growth performance, insulin, blood metabolites and gut microbiota in weaned piglets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222802/
https://www.ncbi.nlm.nih.gov/pubmed/32331306
http://dx.doi.org/10.3390/ani10040726
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