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Ultrastructural Characterization of Porcine Growing and In Vitro Matured Oocytes

SIMPLE SUMMARY: During oocyte growth and maturation, the organelle’s morphology of porcine oocytes changed and populated different compartments depending on the differentiation status. Changes in ultrastructural or subcellular level of porcine oocytes during oogenesis/folliculogenesis were observed,...

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Autores principales: Kere, Michel, Liu, Pan-Chen, Chen, Yuh-Kun, Chao, Pei-Chi, Tsai, Li-Kuang, Yeh, Ting-Yu, Siriboon, Chawalit, Intawicha, Payungsuk, Lo, Neng-Wen, Chiang, Hsing-I, Fan, Yang-Kwang, Ju, Jyh-Cherng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222836/
https://www.ncbi.nlm.nih.gov/pubmed/32290459
http://dx.doi.org/10.3390/ani10040664
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author Kere, Michel
Liu, Pan-Chen
Chen, Yuh-Kun
Chao, Pei-Chi
Tsai, Li-Kuang
Yeh, Ting-Yu
Siriboon, Chawalit
Intawicha, Payungsuk
Lo, Neng-Wen
Chiang, Hsing-I
Fan, Yang-Kwang
Ju, Jyh-Cherng
author_facet Kere, Michel
Liu, Pan-Chen
Chen, Yuh-Kun
Chao, Pei-Chi
Tsai, Li-Kuang
Yeh, Ting-Yu
Siriboon, Chawalit
Intawicha, Payungsuk
Lo, Neng-Wen
Chiang, Hsing-I
Fan, Yang-Kwang
Ju, Jyh-Cherng
author_sort Kere, Michel
collection PubMed
description SIMPLE SUMMARY: During oocyte growth and maturation, the organelle’s morphology of porcine oocytes changed and populated different compartments depending on the differentiation status. Changes in ultrastructural or subcellular level of porcine oocytes during oogenesis/folliculogenesis were observed, potentially leading to future mitochondrion replacement therapies of oocytes. ABSTRACT: This study aimed to investigate ultrastructural changes of growing porcine oocytes and in vitro maturated oocytes. Light microscopy was used to characterize and localize the primordial, primary, secondary, and tertiary follicles. During oocyte growth and maturation, the morphology of mitochondria was roundish or ovoid in shape depending on the differentiation state, whereas their mean diameters oscillated between 0.5 and 0.7 µm, respectively, from primary and secondary follicles. Hooded mitochondria were found in the growing oocytes of the tertiary follicles. In addition to the pleomorphism of mitochondria, changes in the appearance of lipid droplets were also observed, along with the alignment of a single layer of cortical granules beneath the oolemma. In conclusion, our study is apparently the first report to portray morphological alterations of mitochondria that possess the hooded structure during the growth phase of porcine oocytes. The spatiotemporal and intrinsic changes during oogenesis/folliculogenesis are phenomena at the ultrastructural or subcellular level of porcine oocytes, highlighting an in-depth understanding of oocyte biology and impetus for future studies on practical mitochondrion replacement therapies for oocytes.
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spelling pubmed-72228362020-05-18 Ultrastructural Characterization of Porcine Growing and In Vitro Matured Oocytes Kere, Michel Liu, Pan-Chen Chen, Yuh-Kun Chao, Pei-Chi Tsai, Li-Kuang Yeh, Ting-Yu Siriboon, Chawalit Intawicha, Payungsuk Lo, Neng-Wen Chiang, Hsing-I Fan, Yang-Kwang Ju, Jyh-Cherng Animals (Basel) Article SIMPLE SUMMARY: During oocyte growth and maturation, the organelle’s morphology of porcine oocytes changed and populated different compartments depending on the differentiation status. Changes in ultrastructural or subcellular level of porcine oocytes during oogenesis/folliculogenesis were observed, potentially leading to future mitochondrion replacement therapies of oocytes. ABSTRACT: This study aimed to investigate ultrastructural changes of growing porcine oocytes and in vitro maturated oocytes. Light microscopy was used to characterize and localize the primordial, primary, secondary, and tertiary follicles. During oocyte growth and maturation, the morphology of mitochondria was roundish or ovoid in shape depending on the differentiation state, whereas their mean diameters oscillated between 0.5 and 0.7 µm, respectively, from primary and secondary follicles. Hooded mitochondria were found in the growing oocytes of the tertiary follicles. In addition to the pleomorphism of mitochondria, changes in the appearance of lipid droplets were also observed, along with the alignment of a single layer of cortical granules beneath the oolemma. In conclusion, our study is apparently the first report to portray morphological alterations of mitochondria that possess the hooded structure during the growth phase of porcine oocytes. The spatiotemporal and intrinsic changes during oogenesis/folliculogenesis are phenomena at the ultrastructural or subcellular level of porcine oocytes, highlighting an in-depth understanding of oocyte biology and impetus for future studies on practical mitochondrion replacement therapies for oocytes. MDPI 2020-04-11 /pmc/articles/PMC7222836/ /pubmed/32290459 http://dx.doi.org/10.3390/ani10040664 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kere, Michel
Liu, Pan-Chen
Chen, Yuh-Kun
Chao, Pei-Chi
Tsai, Li-Kuang
Yeh, Ting-Yu
Siriboon, Chawalit
Intawicha, Payungsuk
Lo, Neng-Wen
Chiang, Hsing-I
Fan, Yang-Kwang
Ju, Jyh-Cherng
Ultrastructural Characterization of Porcine Growing and In Vitro Matured Oocytes
title Ultrastructural Characterization of Porcine Growing and In Vitro Matured Oocytes
title_full Ultrastructural Characterization of Porcine Growing and In Vitro Matured Oocytes
title_fullStr Ultrastructural Characterization of Porcine Growing and In Vitro Matured Oocytes
title_full_unstemmed Ultrastructural Characterization of Porcine Growing and In Vitro Matured Oocytes
title_short Ultrastructural Characterization of Porcine Growing and In Vitro Matured Oocytes
title_sort ultrastructural characterization of porcine growing and in vitro matured oocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222836/
https://www.ncbi.nlm.nih.gov/pubmed/32290459
http://dx.doi.org/10.3390/ani10040664
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