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Gut microbiota in neonates with congenital gastrointestinal surgical conditions: a prospective study
BACKGROUND: There is limited information on gut microbiota of neonates with congenital gastrointestinal surgical conditions (CGISCs) available. METHODS: This study compared stool microbiota and short-chain fatty acids (SCFAs) of 37 term infants with CGISCs with 36 term healthy infants (HIs). Two sto...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group US
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7223116/ https://www.ncbi.nlm.nih.gov/pubmed/32179871 http://dx.doi.org/10.1038/s41390-020-0824-7 |
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author | Rao, Shripada C. Esvaran, Meera Patole, Sanjay K. Simmer, Karen N. Gollow, Ian Keil, Anthony Wemheuer, Bernd Chen, Liwei Conway, Patricia L. |
author_facet | Rao, Shripada C. Esvaran, Meera Patole, Sanjay K. Simmer, Karen N. Gollow, Ian Keil, Anthony Wemheuer, Bernd Chen, Liwei Conway, Patricia L. |
author_sort | Rao, Shripada C. |
collection | PubMed |
description | BACKGROUND: There is limited information on gut microbiota of neonates with congenital gastrointestinal surgical conditions (CGISCs) available. METHODS: This study compared stool microbiota and short-chain fatty acids (SCFAs) of 37 term infants with CGISCs with 36 term healthy infants (HIs). Two stool samples were collected from each infant: as soon as possible after birth (week 1) and 10–14 days of life (week 2). RESULTS: Bacterial richness and alpha diversity were comparable between CGISCs and HIs at week 1 and week 2 (all p > 0.05). Beta diversity analysis revealed that at week 1, CGISCs had similar community structures to HIs (p = 0.415). However, by week 2, community structures of CGISCs were significantly different from HIs (p = 0.003). At week 1, there were no significant differences in the relative abundances of genera Bifidobacterium and Bacteroides between CGISCs and HIs. At week 2, the relative abundance of Bifidobacterium was significantly lower in CGISCs (mean percentage 7.21 ± 13.49 vs. 28.96 ± 19.6; p = 0.002). Bacteroides were also less abundant in the CGISC group (mean percentage 0.12 ± 0.49 vs. 6.59 ± 8.62; p = 0.039). Relative abundance of genera Pseudomonas and Escherichia–Shigella were higher in CGISCs. At week 2, stool concentrations of all SCFAs were lower in CGISCs (all p < 0.001). CONCLUSIONS: During hospitalization, neonates with CGISCs develop gut dysbiosis and deficiency of SCFAs. IMPACT: During hospitalisation, neonates with congenital gastrointestinal surgical conditions develop gut dysbiosis with deficiency of Bifidobacteria and Bacteroides and increased abundance of Escherichia-Shigella and Pseudomonas. They also have low levels of short chain fatty acids in their stools compared to healthy infants. This is the first study evaluating the gut microbiota using 16S ribosomal RNA sequencing methods and stool short chain fatty acids in neonates with congenital gastrointestinal surgical conditions and comparing them to healthy infants. The findings of this study will pave the way for randomised trials of bifidobacterial supplementation in neonates with congenital gastrointestinal surgical conditions. |
format | Online Article Text |
id | pubmed-7223116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group US |
record_format | MEDLINE/PubMed |
spelling | pubmed-72231162020-05-15 Gut microbiota in neonates with congenital gastrointestinal surgical conditions: a prospective study Rao, Shripada C. Esvaran, Meera Patole, Sanjay K. Simmer, Karen N. Gollow, Ian Keil, Anthony Wemheuer, Bernd Chen, Liwei Conway, Patricia L. Pediatr Res Clinical Research Article BACKGROUND: There is limited information on gut microbiota of neonates with congenital gastrointestinal surgical conditions (CGISCs) available. METHODS: This study compared stool microbiota and short-chain fatty acids (SCFAs) of 37 term infants with CGISCs with 36 term healthy infants (HIs). Two stool samples were collected from each infant: as soon as possible after birth (week 1) and 10–14 days of life (week 2). RESULTS: Bacterial richness and alpha diversity were comparable between CGISCs and HIs at week 1 and week 2 (all p > 0.05). Beta diversity analysis revealed that at week 1, CGISCs had similar community structures to HIs (p = 0.415). However, by week 2, community structures of CGISCs were significantly different from HIs (p = 0.003). At week 1, there were no significant differences in the relative abundances of genera Bifidobacterium and Bacteroides between CGISCs and HIs. At week 2, the relative abundance of Bifidobacterium was significantly lower in CGISCs (mean percentage 7.21 ± 13.49 vs. 28.96 ± 19.6; p = 0.002). Bacteroides were also less abundant in the CGISC group (mean percentage 0.12 ± 0.49 vs. 6.59 ± 8.62; p = 0.039). Relative abundance of genera Pseudomonas and Escherichia–Shigella were higher in CGISCs. At week 2, stool concentrations of all SCFAs were lower in CGISCs (all p < 0.001). CONCLUSIONS: During hospitalization, neonates with CGISCs develop gut dysbiosis and deficiency of SCFAs. IMPACT: During hospitalisation, neonates with congenital gastrointestinal surgical conditions develop gut dysbiosis with deficiency of Bifidobacteria and Bacteroides and increased abundance of Escherichia-Shigella and Pseudomonas. They also have low levels of short chain fatty acids in their stools compared to healthy infants. This is the first study evaluating the gut microbiota using 16S ribosomal RNA sequencing methods and stool short chain fatty acids in neonates with congenital gastrointestinal surgical conditions and comparing them to healthy infants. The findings of this study will pave the way for randomised trials of bifidobacterial supplementation in neonates with congenital gastrointestinal surgical conditions. Nature Publishing Group US 2020-03-16 2020 /pmc/articles/PMC7223116/ /pubmed/32179871 http://dx.doi.org/10.1038/s41390-020-0824-7 Text en © International Pediatric Research Foundation, Inc 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Clinical Research Article Rao, Shripada C. Esvaran, Meera Patole, Sanjay K. Simmer, Karen N. Gollow, Ian Keil, Anthony Wemheuer, Bernd Chen, Liwei Conway, Patricia L. Gut microbiota in neonates with congenital gastrointestinal surgical conditions: a prospective study |
title | Gut microbiota in neonates with congenital gastrointestinal surgical conditions: a prospective study |
title_full | Gut microbiota in neonates with congenital gastrointestinal surgical conditions: a prospective study |
title_fullStr | Gut microbiota in neonates with congenital gastrointestinal surgical conditions: a prospective study |
title_full_unstemmed | Gut microbiota in neonates with congenital gastrointestinal surgical conditions: a prospective study |
title_short | Gut microbiota in neonates with congenital gastrointestinal surgical conditions: a prospective study |
title_sort | gut microbiota in neonates with congenital gastrointestinal surgical conditions: a prospective study |
topic | Clinical Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7223116/ https://www.ncbi.nlm.nih.gov/pubmed/32179871 http://dx.doi.org/10.1038/s41390-020-0824-7 |
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