Comparative thermostability analysis of zoonotic and human influenza virus A and B neuraminidase

Neuraminidase (NA) thermostability of influenza A and B viruses isolated from birds, swine and humans was measured to evaluate its variability associated with host body temperature. The highest 50% inactivation temperature (IT(50)) was observed with H3N8 avian influenza virus (74 °C), and the lowest...

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Detalles Bibliográficos
Autores principales: Evseenko, Vasily A., Svyatchenko, Svetlana V., Kolosova, Natalia P., Kovrizhkina, Valentina L., Marchenko, Vasiliy Y., Durymanov, Aleksander G., Goncharova, Natalia I., Ryzhikov, Alexander B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2019
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7223169/
https://www.ncbi.nlm.nih.gov/pubmed/31745716
http://dx.doi.org/10.1007/s00705-019-04465-w
Descripción
Sumario:Neuraminidase (NA) thermostability of influenza A and B viruses isolated from birds, swine and humans was measured to evaluate its variability associated with host body temperature. The highest 50% inactivation temperature (IT(50)) was observed with H3N8 avian influenza virus (74 °C), and the lowest IT(50) was observed with the seasonal human H3N2 virus (45.5 °C). The IT(50) values of A(H1N1)pdm09 viruses 56.4-58.5 °C were statistically higher than that of the prepandemic strain A/Solomon Islands/03/06 (52.5 °C). An analysis of Ca(2+) binding sites revealed the correspondence of amino acid changes to NA thermostability. This study demonstrates that changes in NA thermostability correspond to differences in host body temperature.

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