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Insight into Evolution and Conservation Patterns of B1-Subfamily Members of GPCR

The diverse, evolutionary architectures of proteins can be regarded as molecular fossils, tracing a historical path that marks important milestones across life. The B1-subfamily of GPCRs (G-protein-coupled receptors) are medically significant proteins that comprise 15 transmembrane receptor proteins...

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Autores principales: Chakraborty, Chiranjib, Sharma, Ashish Ranjan, Sharma, Garima, Bhattacharya, Manojit, Lee, Sang-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7223794/
https://www.ncbi.nlm.nih.gov/pubmed/32421105
http://dx.doi.org/10.1007/s10989-020-10043-5
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author Chakraborty, Chiranjib
Sharma, Ashish Ranjan
Sharma, Garima
Bhattacharya, Manojit
Lee, Sang-Soo
author_facet Chakraborty, Chiranjib
Sharma, Ashish Ranjan
Sharma, Garima
Bhattacharya, Manojit
Lee, Sang-Soo
author_sort Chakraborty, Chiranjib
collection PubMed
description The diverse, evolutionary architectures of proteins can be regarded as molecular fossils, tracing a historical path that marks important milestones across life. The B1-subfamily of GPCRs (G-protein-coupled receptors) are medically significant proteins that comprise 15 transmembrane receptor proteins in Homo sapiens. These proteins control the intracellular concentration of cyclic AMP as well as various vital processes in the body. However, little is known about the evolutionary correlation and conservational blueprint of this GPCR subfamily. We performed a comprehensive analysis to understand the evolutionary architecture among 13 members of the B1-subfamily. Multiple sequence alignment analysis exhibited six multiple sequence aligned blocks and five highly aligned blocks. Molecular phylogenetics indicated that CRHR1 and CRHR2 share a typical ancestral relationship and are siblings in 100% bootstrap replications with a total of 24 nodes observed in the cladogram. CRHR2 has the maximum number of extremely conserved amino acids followed by ADCYAP1R1. The longest continuous number sequence logos (74) were found between sequence location 349 and 423, and consequently, the maximum and minimum logo height recorded was 3.6 bits and 0.18 bits, respectively. Finally, to understand the model and pattern of evolutionary relatedness, the conservation blueprint, and the diversification among the members of a protein family, GPCR distribution from several species throughout the animal kingdom was analysed. Together, the study provides an evolutionary insight and offers a rapid method to explore the potential of depicting the evolutionary relationship, conservation blueprint, and diversification among the B1-subfamily of GPCRs using bioinformatics, algorithm analysis, and mathematical models. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10989-020-10043-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-72237942020-05-15 Insight into Evolution and Conservation Patterns of B1-Subfamily Members of GPCR Chakraborty, Chiranjib Sharma, Ashish Ranjan Sharma, Garima Bhattacharya, Manojit Lee, Sang-Soo Int J Pept Res Ther Article The diverse, evolutionary architectures of proteins can be regarded as molecular fossils, tracing a historical path that marks important milestones across life. The B1-subfamily of GPCRs (G-protein-coupled receptors) are medically significant proteins that comprise 15 transmembrane receptor proteins in Homo sapiens. These proteins control the intracellular concentration of cyclic AMP as well as various vital processes in the body. However, little is known about the evolutionary correlation and conservational blueprint of this GPCR subfamily. We performed a comprehensive analysis to understand the evolutionary architecture among 13 members of the B1-subfamily. Multiple sequence alignment analysis exhibited six multiple sequence aligned blocks and five highly aligned blocks. Molecular phylogenetics indicated that CRHR1 and CRHR2 share a typical ancestral relationship and are siblings in 100% bootstrap replications with a total of 24 nodes observed in the cladogram. CRHR2 has the maximum number of extremely conserved amino acids followed by ADCYAP1R1. The longest continuous number sequence logos (74) were found between sequence location 349 and 423, and consequently, the maximum and minimum logo height recorded was 3.6 bits and 0.18 bits, respectively. Finally, to understand the model and pattern of evolutionary relatedness, the conservation blueprint, and the diversification among the members of a protein family, GPCR distribution from several species throughout the animal kingdom was analysed. Together, the study provides an evolutionary insight and offers a rapid method to explore the potential of depicting the evolutionary relationship, conservation blueprint, and diversification among the B1-subfamily of GPCRs using bioinformatics, algorithm analysis, and mathematical models. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10989-020-10043-5) contains supplementary material, which is available to authorized users. Springer Netherlands 2020-02-08 2020 /pmc/articles/PMC7223794/ /pubmed/32421105 http://dx.doi.org/10.1007/s10989-020-10043-5 Text en © Springer Nature B.V. 2020 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Chakraborty, Chiranjib
Sharma, Ashish Ranjan
Sharma, Garima
Bhattacharya, Manojit
Lee, Sang-Soo
Insight into Evolution and Conservation Patterns of B1-Subfamily Members of GPCR
title Insight into Evolution and Conservation Patterns of B1-Subfamily Members of GPCR
title_full Insight into Evolution and Conservation Patterns of B1-Subfamily Members of GPCR
title_fullStr Insight into Evolution and Conservation Patterns of B1-Subfamily Members of GPCR
title_full_unstemmed Insight into Evolution and Conservation Patterns of B1-Subfamily Members of GPCR
title_short Insight into Evolution and Conservation Patterns of B1-Subfamily Members of GPCR
title_sort insight into evolution and conservation patterns of b1-subfamily members of gpcr
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7223794/
https://www.ncbi.nlm.nih.gov/pubmed/32421105
http://dx.doi.org/10.1007/s10989-020-10043-5
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